The effects of heattreated human bone morphogenetic protein on clinical implantation

1
The effects of heat-treated human bone
morphogenetic protein on clinical
implantation
Hiroyuki Izawa, MT. Yudo Hachiya, MD, PhD. et al
Clinical orthopaedics and related research. 2001
2
Introduction
- Bone allograft - Contamination by
microorganism - Viral infection -
Heat-treatment (HT) - HIV (Human
immunodeficiency virus) - Determination of HT
temperature
3
Introduction (continue)
- Important thing while the HT - BMP (Bone
morphogeneic protein) - Significant this paper
4
Method
In vitro test using sds-page
Bone collected. (from four donors frozen)
Heated (60 ? for 10h) or Non-heated
hBMP extracted from heated and nonheated bone
In vivo test using radiographic he stain
Bone collected nonosseous tissue periosteum
were discarded then BM
removed from femurs, tibias, fibulas.
5
Result
- In vitro (sds-page result)
6
Result (continue)
- In vivo (radiographic analysis)
7
Result (continue)
- In vivo (histologic analysis)
8
Discussion
- sds-page - Stimulate new bone formation -
other temperature of HT - new experiment design
9
Discussion (continue)
Title Sensitive of osteoinductivity of
demineralized and defatted rat femur to
temperature and duration of heating
CB collected. (CBcortical bone)
Heated in water bath (50-100? various time)
Each sample transplanted into the hamstring
muscle
mRNA expression were determined for ALP and
collagen
CB collected from 6-week-old rat femurs
10
Discussion (continue)
For in vitro test osteoblast cell collected
Non-heated 60 ? 10h 70 ? 1h
Assayed by gene expression BMP, ALP, collagen
Human bone collected
For in vivo test hBMP collected
Non-heated 60 ? 10h 70 ? 1h
Implantation on animal Assayed by radiographic,
histology
11
Reference
Izawa H, Hachiya Y, Kawai T, Muramatsu K, Narita
Y, Ban N, Yoshizawa H. (2001) The effect of
heat-treated human bone morphogenetic protein
on clinical implantation. PMID 11550873 PubMed
- indexed for MEDLINE Ito T, Sakano S, Sato K,
Sugiura H, Iwata H, Murata Y, Seo H.
(1995) Sensitivity of osteoinductive activity of
demineralized and defatted rat femur to
temperature and duration of heating. PMID
7634716 PubMed indexed for MEDLINE