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Unique and redundant roles for HOG MAPK pathway components as revealed by wholegenome expression ana

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Unique and redundant roles for HOG MAPK pathway components as revealed by whole ... ?????? ?????? ????? ?????? HOG ?????? ??????????? ?????. ?????? ?????? ... – PowerPoint PPT presentation

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Title: Unique and redundant roles for HOG MAPK pathway components as revealed by wholegenome expression ana


1
Unique and redundant roles for HOG MAPK pathway
components as revealed by whole-genome expression
analysis ORourke and Herskowitz(2003)
2
  • ???? ???? ??????? ?? ????????, ??????? ???????
    ????.
  • ?????? ?????? ??????????? ??????? ?????

???? ?????????? ?????
????? ????? ?????? ?????? ??????? ???
????? ???? ???? ???
????? ????????
????? ????????? ????? ?????? ????? HOG (High
Osmolarity Glycerol)
3
??? ??????? ????? ??????????? ????? Sln1 ? Sho1
  • ?????? ? HOG ?? ??? ????? upstream ????????.

MAP KKKs
MAP KK
MAP K ???????? ?????????? ?????? ??? ?????
?????? ?????.
4
  • ???? ????? ?? ??'???? ?????????
  • ???? ???? ???? ?? ?????? ??????? ?????? ?????? ?
    HOG.
  • ????? ??????? ??????? ???????.
  • ????? ?????? ??????? ?????? ??????????? ?????
    ????? ????? ??????.

5
  • ???? ?????
  • ????? ??????? ?????.
  • ???? ????? HOG1 ?????? ??????????? ?????.
  • ?????? ?????? ????? ?????? HOG ?????? ???????????
    ?????.
  • ?????? ?????? ??????????? ?????.
  • ?????? ?????? ?? ?????? ????? ??? ???? ???"?.

6
  • ?. ??????.
  • ???? ??? wild type,
  • ??? ??????? ??????? 1M ????????, 0.5M KCl.
  • ??? ?????? 10 ??' ??? ?????? ?? 180 ????.
  • ??? ??' ??? "?????".

7
  • ?????? ??? ???????? ??? ?????.
  • 2277 ???? ????? "?????? ??????????".

???? ????? ??? ????. ?????? ? KCl ??????? ?????,
??????? ????????? ??????? ???? ????.
8
???? ????? ????????
???? ???? ??? ???? ? KCl.
  • ???? ???? ?????
  • ??????-KCl
  • ???? ??????-????????.

???? ??' ???? ?????? ?"? 0.5M KCl. ??? ??'
???? ?????? ?"? 1M ????????.
9
???? ?????? ??????
  • ???? ?????? ?? ?????? ????? ????? ???? ?????? ?
    KCl ????? ????????.

???? ???? ?????? ??????? ? 0.5M KCl. ??? ????
?????? ??????? ? 1M ????????.
10
  • ???? ?????? ??????, ???? ?????? ???? ? KCl ?????
    ????? ?????? ?????? ?? ???????? ????? ?? KCl.

11
?. ???? ????? HOG1. ?????? ??? ??????? wild
type ???? hog1? ?????? ??????????? ?????. ???
?? ????? ??????????? ????? ???? ??? ?????-??????
(ME) ??? ????? ??? ?????? ???? ??? ????? ??? ?
wt. ????, ????? ??? ? ME ??? ???? ? 3 ?? ??? ?
, ?????? ?????-HOG1.
12
????? 579 ???? ?????- HOG1 ???????? ????? ??????
6 ?????? ????? ?????? ?????.
??? 2 ???? ??????? ? HOG1 ?????? ?????? ??????
?? ?????? ???? ??????????? ?????. ?????? ????
?????? ????????-3-???????.
??? 6 ???? ?? wt ?????? ????? ????? ??????
??????????? ?????, ?? ?????? ?????? ?????
??????. ?????? ???? ??? ?????? ????????????,
???????? ?????? ????? ??????? ?????? ???????
????? ?????.
??? 5 ??? ???? 4, ?? ????? ?????? ????. ???
????? 4 ???? ???? ???????? ?????? ????? ??????
???????? ?????? ???? ?????? ? stress (ESR).
??? 4 ???? ??????? ???? ??????, ?? ? wt ??????
???? ????? ??????? ?????? ????.
??? 1 ???? ??????? "????? ??-????????"
??????. ?????? ???? ???? ????? ???? ??????
??????? ????????? ?? ? filamentous growth .
??? 3 ???? ??????? ???? ?? ?????? ????? ? wt
?????? ??????????? ?????.
????? 4, 5 ? 6. ????? ???? ?????? ? wt. ?????
????? ??????. ? HOG1 ?? ????? ????? ?????.
13
???? ????? ???????? ??????????? ????? ? wt
?????? ? 40 ????? ????????, ????? ????? ????? ??
???????? ????? ?? ???? 40 ???? ?????? ?????? 0,
10, 20, 30 ? 40 ????.
14
?. ?????? ????? ?????? ? HOG.
?????? ??? ????? ????? ??? ??? ????????
?????. ????? ??????? ??????? ?????? ????????
?????? ??????????? ????? (0.5M KCl).
15
????? ?????? ?????? ?? ???????? hog1? ? pbs2?
???? ????.
????? ?????? ????? ?? ?????? Pbs2 ??? ????? ??
HOG1.
16
????? ? (ssk1?ste11?) ???? ?? ??? ???????
??????? ?????? Pbs2 (? HOG1). ????? ?????? ???
?????? ????? ???? pbs2? ? hog1?.
????? ??????? ? Pbs2 ?? HOG1.
???? ????? ?????.
????? ???? ??? ????? ?????? Pbs2, ???? Ste11 ?
Ssk1.
17
??? ?????? Sho1 ??? ????? ????? ?????? Ste11
??????????? ?????? ????? ??? ??????? ??????
ssk1?ste11? (????)
? ssk1?sho1? (????).
????? ???? ?? ??????? ? Sho1 (????) ????? ????
?????.
????? ???? ????? ???? ????? ?????? Ste11 ??????
??????????? ?????.
18
??? ?? ?????? ?????? ??? ?????? upstream, ??????
??????????? ?????? ????? ????? ??????? ste11?,
ssk1? ? sho1?.
?????? ?????? ??? ???? ?????? ? wt.
????? ?????? ??????????? ?????, ?? ??????
?????? ?? ??? ??????.
19
?. ??? ?? ?????? ?? ???? ??????? ?????? ????
? ??? ????? ??? ????? ??? ??? ?????? ???????
?????? ???????? ?????? ???? 0.0625M KCl ?
0.125M KCl.
20
????? ???????? ?????? ?? KCl ??? ??? ????, ????
????? ???? ?? ????. ?"? ??? ?????? ???????????
???????? ??????.
????? ????. ??????. ???? ?????? ??? ?? ???
???????.
??????? ??????. ???? ?????? ??? Ste11, ??? ?? ???
Ssk1.
21
?. ????? ?????. ??????? ?????? ?????? ??"?
?????? ?? ??? ?????? ???. ???? ?????? ?????
???? (???? ???? ???"?) ? ???? ?? ????? ??????
?????? ???????.
22
???? ?? 0.5M KCl, ????? ?????? ????? ? Ste11 ??
Ssk1 ?? ????, ?? ?? ???? ? 0.123M KCl .
23
????? ???????, ?????? ???????
  • ????? ????? ??????.
  • ??? ????? ????? - 40 ????.
  • ? HOG1 ????? ????? ????? ?????, ?????? ?????.
  • ??? ?????? upstream - ?????? ??????????? ?????,
    ??? ??????????? ????? ?? ??? ??? ????.

24
  • ?????? ??????? ?????? ???? ??? ???? ???? ????.

?
?
25
  • ??? ??? ?????? ??????? ?????? ???????? ????
    ?????? ?????? in vivo.
  • ???????? ?????? ?? Ssk1 ?? ??????? ?????? ??????
    ???? ???????? ??????? ????, ???? ??? ??????? ??
    ??? ???????? ???? ???? ? 0.125M KCl.

?????? ????? ???? ???????
26
  • ????? ?????
  • ????? ????? ???? ?? ???? ???"?.
  • ????? ?? ??'???? ????????? ??? ????? ????? ???
    ????? ????? ????? ???? ??????? ????? ??????.
  • ????? ????? ?"? ????? ?? ?????? ????? ??? ?????.
  • ???? ??????? ?????? ??? ??????? ??????? ??? ??
    ????? ???? ?????/??????
  • - ????? ?? ??? ??? ????? ??? ?? ????? ????
    ?????? ??????? ??? ???????.
  • - ????? ?? ??'???? ????????? ??? ?? ????? ????
    ???' ???? ????????, ????? ????? ??? ???? ?????
    ???.

27
??????
  • Eisen MB et al. (1998) Cluster analysis and
    display of genome-wide expression patterns. Proc
    Natl Acad Sci U S A. 95(25)14863-14868.
  • O'Rourke SM, Herskowitz I. (2004) Unique and
    redundant roles for HOG MAPK pathway components
    as revealed by whole-genome expression analysis.
    Mol Biol Cell. 15(2)532-42.
  • Gasch AP et al. (2000) Genomic expression
    programs in the response of yeast cells to
    environmental changes. Mol Biol Cell.
    11(12)4241-4257.
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