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Damjana Drobne University of Ljubljana, Biotechnical Faculty

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Title: Damjana Drobne University of Ljubljana, Biotechnical Faculty


1
CONFERENCE ON NANO-SAFETY 22.-24. April 2009,
Ljubljana, Slovenia
Nanosized ZnO and nano TiO2 bioavailability and
bioaccumulation
Damjana Drobne University of Ljubljana,
Biotechnical Faculty Department of Biology,
SLOVENIA E-mail Damjana.Drobne_at_bf.uni-lj.si
2
  • Research group for nanobiology and nanotoxicology
  • Dept. Biology, Biotechnical faculty, Uni
    Ljubljana
  • Damjana Drobne Vladka Leer Janez Valant iva
    Tkalec Pipan
  • Marjetka Kralj Kuncic Sara Novak, student Tea
    Romih student
  • ______________________________________________
  • National Institute of chemstry
  • Anita Jemec
  • ______________________________________________
  • Faculty for computer and information science, Uni
    Ljubljana
  • Jernej Zupanc

3
  • J1-9475 (C) Elaboration and evealution of a
    single species toxicity test for nanoparticles
    with a terrestrial isopod evaluation and Izdelava
    in vrednotenje testa strupenosti (basic research
    project), Slovenian Research Agency 2006-2009

4
  • Aim of work
  • . to study interactions between nanoparticles
    and biological systes

5
In vivo and in vitro biological system
Lipid vesicles
A terrestrial isopod, a model invertebrate
organism
6
Our past experiences
... studding toxicity of metal pollutants and
pesticides present in the environment.
7
Present challanges
Interactions between nanoparticles and biological
systems.
8

Photo M. Bele, Institute of chemistry, Ljubljana
.. .biological potential of nanosized metal oxides
9
Chemicals affect target molecules or interfere
with different intracellular compartments. The
effect is propagated upwards to higher levels of
biological complexity (cells, tissue, organs,
organism) and it is manifested as toxic response.

10
What about nanoparticles?
11
1) One hypothesis is that nanoparticles
interact firstwith cell membrane!

A consequesnce is.
12
Photo J. Valant
cells are affected, but nanoparticles were not
detected inside the cells (nanoTiO2).
13
2) The other opened question is are
nanoparticle bioavailable do they
bioaccumulate?
?
By L.Ziccardi, M. McArdle, Y. Lowney, J. Tsuji

14
  • Do ZnO nanoparticles accumulate?

15
  • Our work
  • model organism, terrestrial isopod P. scaber
  • food exposure to nanoparticles for 4 weeks
  • comparison among bioaccumulation of
  • nano ZnO,
  • macropowder ZnO
  • ZnCl2

16
  • Background
  • terrestrial isopods accumulate high amounts of
    meals (Zn, Pb, Cu, Fe etc). in their tissue
  • a lot of knowledge already exist on the
    accumulation pattern and accumulation strategy of
    metals if offered as soluble salts

17
Experimental set up
Four weeks feeding on Nanoparticel, macropowsder
or ZnCl dosed food

metal analyses in whole organism by AAS
concentrations of Zn in the body
18
Background knowledge FIB/SEM,
19
Photo F.Tatti, FEI
20

Scanning electron micrograph of macropowder of
ZnO.
Scanning electron micrograph of nanosized ZnO
21
  • Results
  • no diffrences in amount of accumulated Zn among
    groups

22
5000 µg/g
2000 µg/g
By . Pipan Tkavec, D. Drobne, A.Jemec ,T.Romih,
P.Zidar, M.Bele
23
  • Discussion
  • we are not able to answere the question about
    nanoaprticle bioavailability or bioaccumauation
    pottential
  • the fact is that when nanosized or macrosized ZnO
    is ingetsed an equal prtion of Zn is accumulated
    as when ZnCl2 is inegested

24
  • Present/Future direction of research
  • investigation of accumulation of other
    nanparticles with lower dissolution rates for
    example Cu and Ag nanoparticles
  • Investigation of interaction between membranes
    and nanoparticles

25
Photo by J.Zupanc
Computer aided analysis of lipid vesicles shape
transformations after incubation with
nanoparticles.
26
  • Publications
  • DROBNE, Damjana, JEMEC, Anita, PIPAN, iva. In
    vivo screening to determine hazards of
    nanoparticles nanosized TiO2. Environ. pollut.
    ,2009, issue 4, vol. 157, str. 1157-1164
  • JEMEC, Anita, DROBNE, Damjana, REMKAR, Maja,
    SEPCIC, Kristina, TILER, Tatjana. Effects of
    ingested nano-sized titanium dioxide on
    terrestrial isopods Porcellio scaber. Environ.
    toxicol. chem., 2008, vol. 27, no. 9, str.
    1904-1914,
  • JEMEC, Anita, DROBNE, Damjana, TILER, Tatjana,
    SEPCIC, Kristina. Biochemical biomarkers in
    environmental studies-lessons learnt from enzymes
    catalase, glutathione S-transferase and
    cholinesterase in two crustacean species.
    Environ. sci. pollut. res. int., 2009, 11 str.,

27
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