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The Effects of Freezing on the Properties of Bovine Meniscus

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The Effects of Freezing on the Properties of Bovine Meniscus. Scott Morgan. What Is Meniscus? ... Bovine menisci. Obtained from local slaughterhouse. Medial ... – PowerPoint PPT presentation

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Title: The Effects of Freezing on the Properties of Bovine Meniscus


1
The Effects of Freezing on the Properties of
Bovine Meniscus
  • Scott Morgan

2
What Is Meniscus?
  • Semi-lunar fibrocartilage
  • Lateral
  • Medial

3
Importance?
  • Provide smooth, low friction articulating
    surfaces
  • Serve as shock absorbers and stabilizers
  • When injured and/or removed
  • Degeneration leading to osteoarthritis
  • Knee movement becomes more difficult

4
Why freezing?
  • Literature review (Proctor et al, J of Ortho R,
    1989)
  • Experimental menisci described as fresh
  • Frozen to microtome twice during slicing
  • Menisci are roughly 70 water
  • No studies to determine effect of freezing on
    material properties

5
Materials Methods
Lateral
Medial
  • Bovine menisci
  • Obtained from local slaughterhouse
  • Medial meniscus only ? to reduce variability

6
Methods
  • Treatment Groups ?5
  • Control (Fresh)
  • 4 Freeze/Thaw Cycle (FTC) Group
  • 1 FTC 5 days frozen, 2 days thawed
  • 2 FTC
  • 3 FTC
  • 4 FTC

7
Methods
8
Methods
  • 12 Menisci per treatment
  • Minimum of 5 slices
  • Maximum of 15 slices
  • Thickness measured
  • Dial caliper
  • Sandpaper glued to ends
  • Facilitated gripping in fixture
  • Provided gage length

9
Methods
  • MTS servohydraulic mechanical testing system
  • Computerized data acquisition
  • Custom gripping jig
  • Performed tensile test

10
Methods
  • Tensile test
  • 1 N preload
  • Photograph for later digital analysis for
    specimen dimensions
  • 0.5 mm/min crosshead speed
  • Tensile tested until failure

11
Methods
  • Data analysis
  • Digital image analysis
  • Specimen dimensions from photos
  • Calculate stress strain
  • Plot stress-strain curve

(Wainwright, 1976)
12
Statistical Analysis
  • Compared menisci within each treatment
  • Look for individual variability
  • Compared treatment groups
  • ANOVA
  • Multiple comparisons Tukey-Kramer

13
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14
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15
Conclusions
  • Large variability between specimens
  • Increasing FTCs lead to increasing variabilitity
  • There appears to be little to no effect of
    multiple FTCs on the tensile strength of the
    circumferential collagen fibers

16
Current
  • Changes in the specimens
  • Texture
  • Ease of slicing
  • Creep Relaxation Testing
  • Design of new apparatus
  • Compression indenter
  • New experimental procedure
  • Meniscus from other species
  • Ovine
  • Porcine

17
Acknowledgments
  • Dr. Carolyn Skurla
  • Daniel Bland
  • Zachary Kelm
  • Erik Schaefer
  • Christopher Towe
  • David Webster
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