DNA replication

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DNA replication

• Haixu Tang
• School of Informatics

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DNA replication and heredity
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Mutation rate

• Low
• E.Coli human 1 in 109 bases
• Correction mechanism
• Experiment measure of mutation rate
• Many mutations are deleterious and eliminated by
  natural selection

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Mutation rates are different from protein to
protein
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Reaction of DNA replication
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The semiconservative DNA replication
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Replication forks
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An incorrect model
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Correct model
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Exonucleolytic Proofreading of polymerase
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Why always 5?3?
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Correct model
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Primase
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DNA synthesis of lagging strand
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DNA ligation
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DNA helicase
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Regulated sliding clamp
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A complete picture of lagging strand synthesis
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DNA repair
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DNA replication in eukaryotes
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Initiation of DNA replication
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Origin of DNA Replication

• Bacterial Chromosomes Have a Single Origin of DNA
  Replication
• Eucaryotic Chromosomes Contain Multiple Origins
  of Replication
• Replication Origins in a Simple Eucaryote, the
  Budding Yeast is identified
• The Mammalian DNA Sequences That Specify the
  Initiation of Replication Have Been Difficult to
  Identify

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DNA replication in cell cycle
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Telomerase Replicates the Ends of Chromosomes
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Spontaneous alterations in DNA
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DNA repair
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DNA recombination

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Site-specific recombination