Collaborative Case-based Study of Genetic and Infectious Diseases Using Protein and DNA Simulations - PowerPoint PPT Presentation

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Collaborative Case-based Study of Genetic and Infectious Diseases Using Protein and DNA Simulations

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Genetic disease case studies. Huntington's disease example. Protein analysis tools ... Sample case: Huntington's Disease ... DNA testing for Huntington's. ... – PowerPoint PPT presentation

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Title: Collaborative Case-based Study of Genetic and Infectious Diseases Using Protein and DNA Simulations


1
Collaborative Case-based Study of Genetic and
Infectious Diseases Using Protein and DNA
Simulations
  • Mark Bergland and Karen Klyczek, University of
    Wisconsin-River Falls

2
Overview
  • Description of Case It project
  • DNA analysis tools
  • Genetic disease case studies
  • Huntingtons disease example
  • Protein analysis tools
  • Infectious disease case studies
  • HIV example
  • Web editor/conferencing system

3
Case It! Project
URL for Case It! Home Page http//caseit.uwrf.edu
Contact mark.s.bergland_at_uwrf.edu
Supported by the CCLI program of the National
Science Foundation
4
Case It! Project
  • Electronic framework for analyzing and discussing
    case studies in molecular biology
  • Genetic and infectious diseases and associated
    ethical issues
  • Students gather background information on cases
  • Analyze DNA and/or protein sequences using
  • Case It! Version 5.03
  • Prepare web-page poster using automated system
  • Poster session via Internet conferencing
  • Role-playing

5
Case It! Simulation
  • Features of Case It! Version 5.03
  • DNA and protein electrophoresis
  • Restriction enzyme digestion and mapping
  • Southern blotting
  • Dot blotting
  • Polymerase Chain Reaction (single and 96-well)
  • ELISA
  • Western blotting

New grant project
  • Add simulations of microarrays, RT-PCR
  • Connect simulations to bioinformatics tools and
    databases
  • Facilitate open-ended research questions

6
Case It! web editor
  • Features of the Case It! web editor
  • Web page construction
  • Conferencing/discussion board
  • Collaborate with classes at other institutions
  • Instructors can add classes
  • Upload class roster, organize teams

7
Sample case Huntingtons Disease
  • Susan is a 23-year-old whose father, age 55, and
    paternal aunt, age 61, have been diagnosed with
    Huntingtons chorea. A paternal uncle, age 66,
    appears to be unaffected by the disease. Susan
    wants to know if she inherited the mutated gene
    from her father so that she can prepare for that
    future if necessary. She arranges to undergo DNA
    testing for Huntingtons. Her 17-year old
    brother, John, also decides to be tested after
    talking with Susan.

8
Ethical issues raised by the case
  • Should someone with a family history of HD be
    required to undergo testing? Should they have
    children if they test positive, or if they have
    not been tested?
  • Should someone as young as John be tested?
  • Should the results of such tests be made
    available to insurance companies? to potential
    employers? to potential mates?

9
Case analysis
  • Case It! Investigator can be used to gather
    background information on case
  • Case It! Version 5.03 is used to analyze DNA
    sequences associated with case
  • Case It! Launch Pad gives access to a web
    editor/conferencing system
  • -electronic poster sessions
  • -role playing
  • Online tutorial gives detailed instructions

10
Protein module
  • ELISA (Enzyme immunoassay)
  • Protein gel electrophoresis/ Western blot
  • Infectious disease cases
  • HIV
  • SARS
  • West Nile virus
  • Influenza
  • Ebola

11
ELISA test results
12
HIV viral proteins
13
Western Blot Band pattern interpretation
Lane 1, HIV serum (positive control) Lane 2,
HIV- serum (negative control) Lane A, Patient A
Lane B, Patient B Lane C, Patient C
Band interpretation
from www.biology.arizona.edu
14
HIV Case studies
  • Case scenario - video and text (HIV Anna),
    using Case It web site
  • ELISA test - initial screening
  • Western blot to follow up ELISA results
  • PCR to measure viral load
  • Upload graphical results to Case It web editor

15
Comments from Zimbabwe students
  • To me it was an eye opener Since Zimbabwe is
    hard hit by HIV it is important to have this
    project CASE IT since it affects the students
    lives at large. The videos shown are touching.
    These can help students feel it.
  • It was also interesting to network with
    international students via conferencing. Indeed,
    the world is a global village.
  • (I) was able to get an understanding of how
    American families react to their family members
    when they have tested HIV positive

16
Comments from Zimbabwe students
  • I had no idea how I could do practicals on H.I.V
    testing given the limited apparatus and financial
    limitations in my school.
  • I found the use of the computer very useful in
    simulations because it enables the carrying out
    of experiments which would otherwise be
    impossible due to the costs involved

17
Comments from Zimbabwe students
  • The programme was also very interesting because
    it had cases of real people that got infected in
    different ways and situations and the
    counselling they were given and how some observed
    the advice and some ignored this advice got
    themselves into worse trouble.

18
Comments from Zimbabwe students
  • The subject still remains every teachers
    concern. Of particular concern however, is the
    increase in the number of orphans. It is
    estimated that by the year 2005 approximately
    910000 children will have lost their parents to
    AIDS. Poverty, depression, and stigma will haunt
    most of these children. They will most likely
    expose themselves to HIV infections in pursuit of
    basic need and will painfully erode their basic
    right to family, protection health and food. Our
    challenge is therefore to intensify awareness
    education to these children.

19
Invitation to participate
  • We welcome participation in the Case It! Project
  • No cost to educators
  • High school and university educators are invited
  • Contact mark.s.bergland_at_uwrf.edu
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