Cell and Molecular Biology Lab BS 111L Spring 2006 Sections: 25 (Thurs 11:30-2:20), 27 (Thurs 3:00-5:50), and 30 (Thurs 7:00-9:50

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Cell and Molecular Biology LabBS 111L Spring
2006Sections 25 (Thurs 1130-220), 27 (Thurs
300-550), and 30 (Thurs 700-950

• Introduction
• Instructor Scott Butterfield
• 6th year Ph.D. Candidate in Plant Biology
• E-mail butter12_at_msu.edu
• Website http//www.msu.edu/butter12/
• Help Hours Thurs. 10-11 2-3 (218 NKL)
• Recitation Fri. 910-1000 am

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Outline For Today

• Lab 1 Part 1 Survey of Lab Techniques
• Laboratory Safety and Video (Safety Agreement)
  (pgs. 12-14)
• Microbial Streaking of TSA Plates (pgs. 15-16)
• Micropipet Techniques (handout and pgs. 17-19)
• Centrifugation (pgs. 20-21)
• 5) Graphing Standard vs. Optimal vs. Time
  course curves (pages 33-34)

3
Streaking and Evaluation of the TSA Plate
Cultures (pgs. 15-16)
TSA Tryptic soy agar
Confluency Streaking
Isolation Streaking (3 Zone)
1
2
3
Colonies
Lawn

• 2 different species of bacteria
• Staphylococcus aureus (appears white)
• Serratia marcescens (appears red)

4
Graphing (pgs. 33-34)
Three types of basic graphs
FIGURE 1. TITLE

• Time course curve
• Standard curve
• Optimal curve

Y-axis
X-axis

• You will be using Windows Excel 2000
• You will use these curve types to help summarize
  your results
• You will use these graphs throughout the
  semester especially in the enzyme lab (Lab Report
  Lab)