Post Weaning Multisystemic Wasting Disease Syndrome PMWS - PowerPoint PPT Presentation

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Post Weaning Multisystemic Wasting Disease Syndrome PMWS

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Provide diagnostic tips and comments for achieving maximum value ... Make a tab of skin beginning under the mandibles. Iowa State College of ... photography ... – PowerPoint PPT presentation

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Title: Post Weaning Multisystemic Wasting Disease Syndrome PMWS


1
Swine Postmortem Techniques for Grow
Finish Locke Karriker DVM, MS Pat Halbur, DVM,
PhD Iowa State University Veterinary Diagnostic
Laboratory
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Objectives for this session
  • Use images to review step-by-step procedures for
    necropsy procedure
  • Identify gross abnormalities of respiratory
    (lungs) and enteric systems (digestive track)
  • Understand the collection of appropriate tissue
    specimens for diagnostic investigation
  • Provide diagnostic tips and comments for
    achieving maximum value from diagnostic lab
    submissions

4
Reflect front and rear legs
Iowa State College of Veterinary Medicine
5
Make a tab of skin beginning under the mandibles
Iowa State College of Veterinary Medicine
6
Pull up on the tab and reflect skin while cutting
through the sternum.
Iowa State College of Veterinary Medicine
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Iowa State College of Veterinary Medicine
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Iowa State College of Veterinary Medicine
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Iowa State College of Veterinary Medicine
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Iowa State College of Veterinary Medicine
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Iowa State College of Veterinary Medicine
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Iowa State College of Veterinary Medicine
13
Colon
cecum
ileum
Locate ileocecocolic junction
Iowa State College of Veterinary Medicine
14
Identify the ileocecocolic junction
String out the small intestine by cutting the
mesentery
Iowa State College of Veterinary Medicine
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Porcine Enteritis Nursery and Grow-Finish
  • Ileum Two 10 cm sections fresh/chilled, four 1
    cm sections fixed
  • Jejunum Two 10 cm sections fresh/chilled, four 1
    cm sections fixed
  • Cecum and colon Entire organ or two 10 cm
    segments of the spiral colon fresh/chilled, four
    1 cm pieces fixed
  • Lesions 10 cm segment fresh, several pieces
    fixed
  • Feces 10 ml chilled
  • Mesenteric lymph node Fresh and fixed
  • Liver ¼ of organ fresh, 3 slices fixed
  • Stomach Examine for and submit lesions

28
cecum
ileum
jejunum
1 cm sections for histopath
Iowa State College of Veterinary Medicine
29
Thickened small intestinal mucosa in a case of
PCV2-associated enteritis
Enlarged mesenteric lymph nodes
Iowa State College of Veterinary Medicine
30
Nursery-Finisher Enteritis Sampling Tips and
Comments
  • Collect intestines after all other organ samples
    are collected to avoid fecal contamination
  • Package small intestines separate from large,
    package GI tissues separate from all other
    tissues
  • Samples must be taken within minutes of death to
    minimize autolysis of villi
  • Flush intestinal contents out of histopath
    sections and expose mucosa to formalin
  • In cases of necrotic enteritis, submit necrotic
    segments and adjacent non-necrotic segments

31
Porcine Pneumonia
  • Brain ½ fresh/chilled and ½ fixed
  • Upper respiratory tract
  • Swab of turbinate
  • Swab of bronchus
  • Turbinate scroll fixed
  • Lung
  • Bronchoalveolar lavage fluid if PRRSV VI
    requested
  • Entire side with no holes or generous portion (10
    cm cube) with lesion submitted fresh
  • Five 1 cm slices formalin-fixed…see map
  • Tracheobronchial lymph node ½ fresh, ½ fixed
  • Tonsil ½ fresh and ½ fixed

32
Nasal swabs can be taken from live or euthanized
pigs. Use appropriate swabs (viral or bacterial)
and get sample from the middle region of the
nasal turbinate.
Iowa State College of Veterinary Medicine
33
A hack saw is used to cut the snout for a
transverse view
Turbinate atrophy
Septal deviation
Iowa State College of Veterinary Medicine
34
Necropsy approach in a finisher pig with
respiratory disease
Iowa State College of Veterinary Medicine
35
Ventral approach
Lateral approach
Iowa State College of Veterinary Medicine
36
View of the carcass after removal of sternum and
ventral abdominal skin and cracking the ribs back
Iowa State College of Veterinary Medicine
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View of the carcass on which a lateral approach
was used and sets of 2-3 ribs were cracked back
Iowa State College of Veterinary Medicine
38
Remove larynx with the pulmonary tract pluck
Larynx
Ventral head/mandible
Iowa State College of Veterinary Medicine
39
Trachea and lymph nodes
Thoracic inlet
Lung
Iowa State College of Veterinary Medicine
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1
2
Include affected and adjacent unaffected tissue
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5
Location for collection of 5 slices of lung for
histopath
Include airway cross sections
Iowa State College of Veterinary Medicine
41
Lung from a pig experimentally infected with
porcine circovirus type 2
Collect tracheobronchial lymph nodes for
microbiology and histopath
Iowa State College of Veterinary Medicine
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1. Carefully cut through the skin and just into
the joint capsule
2. Using torque, pop open the joint and collect
joint fluid on a swab or in a syringe
Collect synovium for histopath exam
Iowa State College of Veterinary Medicine
55
mediastinal LN
iliac LN
inguinal LN
Collect lymph nodes for microbiological testing
and histopath exam
Iowa State College of Veterinary Medicine
56
Porcine dermatitis and nephropathy syndrome
Salmonellosis
Examine skin and collect lesions in different
stages of progression
Parvovirus and Staphylococcus aureus
Erysipelas
Iowa State College of Veterinary Medicine
57
Acknowledgements
  • ISU-VDL Pathology Section Faculty
  • Dr. Pat Halbur
  • Dr. Kent Schwartz
  • Dr. Mike Yaeger
  • Dr. Dave Larson
  • Dr. Bruce Janke
  • ISU-VDL Director
  • Dr. Gary Osweiler
  • ISU-VDL Necropsy Floor Staff
  • Ray Grover
  • Mike Jennings
  • Jeff Meister
  • ISU Biomedical Communications
  • Jim Fosse for photography

58
Thanks and good luck with your diagnostic
investigations…
http//www.vetmed.iastate.edu/departments/vdpam/vd
l/userguide/pathology
Iowa State College of Veterinary Medicine
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