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New Sol-gel Calcium Phosphate Composites for Bone Regeneration

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Introduction The sol-gel synthesis method ... 24, 48 and 72 h XRD-patterns of OB powder samples 9000C 13000C SiO2 5 wt% SiO2 10 wt% SiO2 15 wt% x OB01 13000C x ... – PowerPoint PPT presentation

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Title: New Sol-gel Calcium Phosphate Composites for Bone Regeneration


1
New Sol-gel Calcium Phosphate Composites for Bone
Regeneration
  • Laura Epure1,2, Fatiha Chellat 1,2, Mariam
    Zoulgami 1
  • Nelson Camargo 3, Yahye Merhi 2, L'Hocine Yahia 1

1 Biomedical Engineering Institute, Laboratory
for the Inovation and Analysis of Bioperformance,
École Polytechnique de Montréal, Canada 2
Laboratory of Experimental Pathology , Montreal
Heart Institute , Canada 3 Universidade do
Estado de Santa Catarina, Centro de Ciencias
Tecnologicas, Brezil
2
Introduction
Calcium phosphate bioceramics
  • The most used calcium phosphate-based bioceramics
    are
  • hydroxyapatite HAp, Ca10(PO4)6(OH)2, and
  • ß-or a- tricalcium phosphate TCP, Ca3(PO4)2.
  • These ceramics are used in porous, granular and
    denses forms as bone graft substitutes in
    orthopedics and dental surgery
  • Poor mechanical properties ? limit their
    application to small unloaded implant, powder,
    surface-coating materials and low-loaded
    implants.
  • Preparation of calcium phosphate-based ceramic
    composites can partially solve the problem of
    these limits.

3
Introduction
Sol-gel synthesis
  • The sol-gel synthesis method is simple, economic,
    and results in a high quality powder which could
    be useful for hard tissue reconstruction
    applications.
  • Processing of sol-gel TCP usually results in a
    fine-grain microstructure and crystals, better
    accepted by the host tissue.

The morphology of the gel powders after
calcination at 9000C TCP
4
Objective
  • To investigate the potential use of new calcium
    phosphate composites obtained by sol-gel method.
  • The study focuses on
  • phase identification,
  • microstructural examination,
  • and biocompatibility evaluations
  • of these new materials.

5
Materials
  • Sol-gel method
  • Reactants for TCP-based ceramic composites
  • SiO2n nano particles lt 100nm , CaO, P2O5 ,
  • CaO calcination of CaCO3 at 9000C for 2 h
  • pH permanently adjusted at 7 (CaP ratio1.67)

Mat. Matrix Reinforcements Wt Traitement
TCP composites powders with SiO2n particles reinforcement TCP composites powders with SiO2n particles reinforcement TCP composites powders with SiO2n particles reinforcement TCP composites powders with SiO2n particles reinforcement TCP composites powders with SiO2n particles reinforcement
OB00 TCP matrix - - 9000C
OB01 TCP matrix - - 13000C
OB1 TCP matrix SiO2 5 9000C
OB2 TCP matrix SiO2 5 13000C
OB3 TCP matrix SiO2 10 9000C
OB4 TCP matrix SiO2 10 13000C
OB5 TCP matrix SiO2 15 9000C
OB6 TCP matrix SiO2 15 13000C
6
Methods
  • Phase identifications of the calcined and
    sintered materials were determined using X-ray
    diffraction (RDX) method.
  • Microstructural examination was determined by
    scanning electron microscopy (SEM)
  • Cell viability was evaluated using MTT
    (3-(4,5-dimethylthiazole-2yl)-2,5-triphenyl
    tetrazolium) coloration assay.

7
Methods
  • Cytotoxicity was studied using the material
    particles extracts.
  • Material particles extracts immersion of the
    composite OB particles
  • in culture medium (0.1 mg/ml)
  • at 370C
  • for a period of 120 h
  • under permanent shaking at 240 rpm.

8
Results
XRD-patterns of OB powder samples
SiO2 wt 5 5 10 10 15 15
Temperature 9000C 13000C 9000C 13000C 9000C 13000C 9000C 13000C
Samples OB00 OB01 OB1 OB2 OB3 OB4 OB5 OB6
TCP beta x x x x x x x x
Si O2 x x x x x x
9
Results
SEM-microstructural examination
10
Results
MTT-cytotoxicity test
  • exposure of L-929 to TCP-SiO2 composites extracts
    did not affect cell viability

11
Conclusions
  • Based on phase identification, microstructural
    examination and biocompatibility testing
  • the new TCP sol-gel composites seem to be good
    biomaterial candidates
  • the results are encouraging but more studies are
    necessary.

Future Work
  • Further investigations are ongoing
  • to study osteoblastic adherence to the tricalcium
    phosphate sol-gel composites and their behaviour
    in a simulated physiologic environment.

12
Thank You !
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