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Gram-Positive Bacilli Part Four

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Nocardia: Laboratory Diagnosis Gram-positive filamentous bacilli Suspicious for actinomycetes Nocardia: Laboratory Diagnosis Cultural characteristics Chalky, matte ... – PowerPoint PPT presentation

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Title: Gram-Positive Bacilli Part Four


1
Gram-Positive BacilliPart Four
  • MLAB 2434 Microbiology
  • Keri Brophy-Martinez

2
Non-Spore Formers Branching
  • Gram positive rod
  • Non-spore formers
  • Pleomorphic morphology
  • Branching
  • Genera
  • Actinomyces
  • Nocardia
  • Streptomyces

3
Aerobic Actinomyces
  • Member of the normal flora of mouth, head, and
    neck
  • Pathogens
  • Human bite wounds, eye cultures
  • Cervicitis and endometritis in women
  • Actinomycosis

4
Aerobic Actinomyces
  • Gram stain
  • Gram positive rod
  • Filamentous, branching
  • Can be beaded
  • Non acid-fast

5
Aerobic Actinomyces
  • Colony morphology
  • Spider or granular center
  • Branching filaments
  • Takes up to 7-14 to develop
  • Key Biochemicals
  • Catalase negative
  • Nonmotile
  • Non-fermenters
  • Produce H2S

6
Aerobic Actinomycetes
  • Nocardia
  • Streptomyces
  • Actinomadura
  • Gordonia
  • Rhodococcus
  • Tsukamurella
  • Nocardiopsis
  • Dermatophilus

7
NocardiaClinical Significance
  • Pulmonary form
  • Mostly in immunocompromised
  • High fatality
  • Starts as lung lesion
  • Cutaneous
  • Three forms
  • Mycetoma
  • Lymphocutaneous
  • Superficial

Sulfur granules collected from draining sinus
tracts in mycetoma
8
NocardiaLaboratory Diagnosis
  • Microscopy
  • Gram-positive branching filaments
  • May show beading appearance
  • Verify with acid fast stain
  • Weakly Acid-fast

Gram-stained smear of sputum showing
Gram-positive branched beaded bacilli.
9
NocardiaLaboratory Diagnosis
  • Gram-positive filamentous bacilli
  • Suspicious for actinomycetes

10
NocardiaLaboratory Diagnosis
  • Cultural characteristics
  • Chalky, matte, dry, crumbly appearance
  • May be pigmented
  • Beta hemolytic
  • Identification
  • Urease positive
  • Catalase positive
  • Molecular testing

11
Streptomyces
  • Habitat
  • Soil and decaying vegetation
  • Disease states
  • Mycetoma- a chronic, localized, painless,
    subcutaneous infection
  • Sites head and neck
  • Wound Skin infections

12
StreptomycesLaboratory Diagnosis
  • Microscopic Morphology
  • Gram positive rod
  • Branching
  • Spider-like
  • Non-acid-fast

13
StreptomycesLaboratory Diagnosis
  • Morphology Characteristics
  • Aerobic growth in 3-30 days
  • Waxy, bumpy or velvety rugose forms, yellow to
    orange
  • Will grown on SBA, mycology media and LJ media
  • GPR with extensive branching, chains and spores
  • Identification
  • Acid-fast negative

14
References
  • http//www.atsu.edu/faculty/chamberlain/golden2000
    /case5.htm
  • http//chesschumpion.blogspot.com/2007/03/time-to-
    put-on-your-thinking-caps.html
  • http//en.wikipedia.org/wiki/FileBacillus_subtili
    s_Gram.jpg
  • https//labs.uhstx.com/clinical_int/dols/appb.html
    http//www.iccb.state.il.us/pt3/mod/science/mod_bi
    o111/mod10/p4.html
  • http//www.flickr.com/photos/microbeworld/sets/721
    57625392265538/detail/http//www.uaz.edu.mx/histo/
    pathology/ed/ch_9b/c9b_clue.htm
  • Kiser, K. M., Payne, W. C., Taff, T. A. (2011).
    Clinical Laboratory Microbiology A Practical
    Approach . Upper Saddle River, NJ Pearson
    Education.
  • Mahon, C. R., Lehman, D. C., Manuselis, G.
    (2011). Textbook of Diagnostic Microbiology (4th
    ed.). Maryland Heights, MO Saunders.
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