Microwave non-destructive testing technique for characterization of HPMC-PEG 3000 films

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Microwave non-destructive testing technique for
characterization of HPMC-PEG 3000 films

• Nor Khaizan Anuar1,3, Wong Tin Wui1,3, Mohd
  Nasir Taib2,3 and Deepak K. Ghodgaonkar4
• 1Particle Design Research Group, Faculty of
  Pharmacy, Universiti Teknologi MARA, 40450, Shah
  Alam, Selangor, Malaysia
• 2Faculty of Electrical Engineering, Universiti
  Teknologi MARA, 40450, Shah Alam, Selangor,
  Malaysia
• 3Non-Destructive Biomedical and Pharmaceutical
  Research Centre, Universiti Teknologi MARA,
  40450, Shah Alam, Selangor, Malaysia
• 4Dhirubhai Ambani Institute of Information and
  Communication Technology, DA-IICT Near Indroda
  Circle, Gandhinagar, 382007, Gujarat, India
• wongtinwui_at_salam.uitm.edu.my

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CONTENT

• 1.0 INTRODUCTION
• 2.0 EXPERIMENTAL
• 2.1 Materials
• 2.2 Sample preparation
• 2.3 Physicochemical characterization
• 3.0 RESULTS AND DISCUSSION
• 4.0 CONCLUSION
• ACKNOWLEDGEMENT
• REFERENCES

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1.0 INTRODUCTION

• Transdermal drug delivery system (TDDS) utilizes
  the skin for the delivery of drug molecules from
  the surface of the skin, through its layers, to
  the circulatory system.
• Quality control of matrix characteristics, such
  as state of polymer-polymer and drug-polymer
  interaction, is essential with respect to the
  therapeutic effectiveness of a TDDS.

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• In the pharmaceutical industry, the analytical
  techniques such as differential scanning
  calorimetry (DSC) and Fourier transform infra-red
  spectroscopy (FTIR) have long been employed to
  determine the matrix characteristics of a TDDS.
• However, these techniques result in sample being
  unrecoverable from test and restrict the analysis
  to statistically selected samples.

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• The present study sets to explore the
  applicability of microwave NDT technique as an
  optional tool to characterize the matrix property
  of polymer film for use as a transdermal drug
  delivery system.

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2.0 EXPERIMENTAL

• 2.1 Materials
• Hydroxypropylmethylcellulose (HPMC, Dow Chemical
  Company, USA) matrix polymer.
• Loratadine (Morepen Laboratories, India) model
  drug.
• Polyethylene glycol (PEG 3000, Merck, Germany)
  plasticizer.

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• 2.2 Sample preparation
• The films were prepared using solvent evaporation
  method.
• The films were conditioned in a desiccator at 25
  ? 1 C and at three different levels of relative
  humidity (25 ? 5 , 50 ? 5 and 75 ? 5 ) for at
  least 5 days prior to the physicochemical
  characterization.

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Sample HPMC (mg) PEG 3000 (mg) Loratadine (mg)
H0 37.5 0 0
P0 37.5 3.75 0
P1 37.5 3.75 5
P2 37.5 3.75 20
Table 1 Theoretical contents of HPMC, PEG 3000
and loratadine in films.
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• 2.3 Physicochemical characterization
• The formed film was subjected to drug content
  assay using UV spectrophotometry technique, DSC,
  FTIR and microwave NDT analysis.

Fig. 1 Rectangular dielectric waveguide (RDWG)
measurement system.
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3.0 RESULTS AND DISCUSSION
i) Drug content analysis
Sample Film thickness (mm) Relative humidity () Relative humidity () Relative humidity ()
Sample Film thickness (mm) 25 50 75
Sample Film thickness (mm) Loratadine content (w/w) Loratadine content (w/w) Loratadine content (w/w)
H0 0.031 ? 0.006 0 0 0
P0 0.036 ? 0.003 0 0 0
P1 0.064 ? 0.015 12.15 ? 0.32 13.59 ? 0.36 13.06 ? 0.16
P2 0.114 ? 0.033 39.06 ? 0.93 35.06 ? 1.14 38.04 ? 1.34
Table 2 Drug content of films measured using the
UV spectrophotometry technique.
The drug content of films was not affected by the
level of relative humidity in the storage chamber
(ANOVA p gt 0.05).
A flat film was formed.
A thicker film was formed in sample containing a
higher content of drug load.
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ii) DSC analysis
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iii) FTIR analysis
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iv) Microwave NDT analysis
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• From the previous study of our laboratory, it was
  found that the measurement of microwave NDT test
  at 8 GHz was sensitive to the chemical
  environment involving polar moiety such as O-H
  functional group, while it was greatly governed
  by the less polar C-H moiety in test conducted at
  12 GHz.
• The present findings indicated that the changes
  of both polar and apolar environments of HPMC-PEG
  3000 films were reflected accordingly by the
  microwave NDT measurements conducted at the
  frequency bands of 8 and 12 GHz respectively.

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4.0 CONCLUSION

• The measurements of microwave NDT test at 8 and
  12 GHz were sensitive to the changes of chemical
  environment in matrix involving polar functional
  group such as O-H moiety and less polar
  functional group such as C-H and aromatic CC
  moieties.
• The present investigation verified that the
  microwave NDT technique has the capacity to
  evaluate the state of interaction between
  polymer, plasticizer and/or drug of a binary
  polymeric matrix, in addition to the existing DSC
  and FTIR techniques.

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ACKNOWLEDGEMENT

• The authors wish to express their heart-felt
  gratitude to Institute of Research, Development
  and Commercialization, UiTM for financial support
  and motivation given throughout the research
  project.

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REFERENCES

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THANK YOU