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Optical Biosensors

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Optical Biosensors Joel Kindt Lauren Netherton Acknowledgements Dr. Kevin Lear Dr. Dave Kisker Weina Wang Hailey Cutler * * Lauren Netherton Motivation and ... – PowerPoint PPT presentation

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Title: Optical Biosensors


1
  • Optical Biosensors
  • Joel Kindt
  • Lauren Netherton
  • Acknowledgements
  • Dr. Kevin Lear
  • Dr. Dave Kisker
  • Weina Wang
  • Hailey Cutler

2
 
  • Lauren Netherton
  • Motivation and Introduction
  • Prior Research
  • How Optical Biosensors Work

3
 
60 of Golden Retrievers Die of Cancer 1 in 4
Dogs Will Die of Cancer -Morris Animal Foundation
Courtesy of Kian Behbakht of UC Denver
4
 
Prior Research Senior Design Team 2007-2008
5
 
  • Pipe Organ Analogy
  • Place fruit in organ pipe
  • Changes pitch based on
  • Size
  • Shape

http//lib.colostate.edu/archives/phelps/
6
Optical Detection RF Switch Circuit
Spectrometer
Courtesy of Weina Wang
7
  • Trapping Requirements
  • Lower voltages
  • Trap at
  • slower velocity
  • Higher voltages
  • Trap at
  • higher velocity

8
 
  • Dielectrophoretic Force
  • Use of electromagnetics
  • Non-uniform electric field
  • Polarizes molecules within cell
  • Induces DEP force to hold cell in trap

Electric field
December 2007 Senior Design Presentation
9
  • Dielectrophoretic Force

Polarization factor (K factor)
  • Complex permittivity of particle/suspending
    medium

Courtesy of Weina Wang
10
 
  • Joel Kindt
  • Electrostatic Modeling
  • Experimental Data
  • Budget
  • Conclusions and Future Work

11
 
Electrodes on Chip
12
 
Electrostatic Modeling
13
 
DEP Force
E-Field
14
  • Positive and Negative DEP
  • Mammalian white blood cell
  • sm 0.1 S/m ( water)
  • sm 1.5 S/m ( PBS)

Polarization factor (K factor)
,
  • K gt 0 Positive DEP
  • K lt 0 Negative DEP

Joel Voldman, Electrical Forces For Microscale
Cell Manipulation, Annu. Rev. Biomed. Eng. 2006.
8425-54
15
 

Experimental Setup
16
 
Impedance of Channel Voltage
Divider http//en.wikipedia.org/wiki/Voltage_div
ider
17
 

Impedance of Channel
? more heat dissipated at lower impedance
18
 
Budget
Materials 1st semester budget 1st semester actual 2nd semester budget
Syringes 15 0 15
Glass 75 0 0
Nanoports 0 0 60
Nanotubing 0 0 25
Flexible mirror 10 0 0
Total 100 0 100
19
 
Conclusions
  • Know trapping requirements for various fluid
    velocities
  • Possible to model cell trap through simulations
  • Electric field
  • DEP force
  • The fluid properties can affect results
  • K factor
  • Impedance of channel

20
 
Future Work
  • Design better cell trap through simulations
  • Further understand spectra
  • Cancerous vs. non-cancerous cells
  • Different types of cancer
  • Save Rover!
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