Liquid Chromatography

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Liquid Chromatography

• Introduction to Liquid Chromatography
• Instrumentation
• 1. Solvent gradient systems
• 2. Injection systems
• 3. Pumps
• 4. Detectors
• 5. Columns
• C. Types of Liquid Chromatography
• Ion-exchange
• Partition chromatography
• Size exclusion chromatography

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Applications of Liquid Chromatography
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Binary Solvents
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Injection Systems on HPLC
Why is an internal standard always necessary in
GC but not in LC?
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HPLC Pumps Most expensive part on HPLC

• Requirements

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Common Types of Pumps

• Gas Displacement Syringe Pumps

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Pneumatic Amplifier Pump D\Data\FTP\movies\pump.m
ov
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Reciprocating Pump
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Columns
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Effect of Particle Size
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HPLC Detectors
Detector Sensitivity Notes