Cytogenetics - technologies,markets and companies

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Cytogenetics - Technologies,Markets and
Companies
Bharat Book Bureau
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Summary

• This report deals with cytogenetics in a
  broader sense rather than the classical use
  mainly to describe the chromosome structure and
  identify abnormalities related to disease. In the
  age of molecular biology, it is also referred to
  as molecular cytogenetics. Historical landmarks
  in the evolution of cytogenetics are reviewed
  since the first images of chromosomes were made
  in 1879. The scope of cytogenetics includes
  several technologies besides fluorescence in situ
  hybridization (FISH), comparative genomic
  hybridization (CGH), and multicolor FISH.
  Molecular cytogenetics includes application of
  nanobiotechnology, microarrays, real-time
  polymerase chain reaction (PCR), in vivo imaging,
  and single molecule detection. Bioinformatics is
  described briefly as it plays an important role
  in analyzing data from many of these
  technologies.

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Continue

• FISH remains the single most important
  technology in cytogenetics. Several innovations
  are described of which the most important are
  single copy FISH, in vivo FISH (imaging of
  nucleic acids in living cells) and
  nanotechnology-based FISH. The unique character
  of peptide nucleic acid (PNA) allows these probes
  to hybridize to target nucleic acid molecules
  more rapidly and with higher affinity and
  specificity compared with DNA probes. PNA-FISH is
  more suited for rapid diagnosis of infections.
  RNA-FISH and locked nucleic acids (LNAs), are
  also described.

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Table of Contents

• 0. Executive Summary ...... 101.
  Introduction . 12Definitions .12Historical
  evolution of cytogenetics ...12Scope of
  cytogenetics 13Molecular cytogenetics
  ......14Basics of molecular biology relevant to
  cytogenetics .14DNA ....... 14RNA .......
  15DNA transcription..... 15Chromosomes .
  15Mitochondrial DNA.... 16Genes..... 16

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