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Bioengineering the Future with Shell Oil Transport Fuels 75

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Bioengineering the Future with Shell Oil Transport Fuels 75% of liquid fuels are used for transport. 70% of that in cars. Retail fuel is 95% fossil alkanes and 5% ... – PowerPoint PPT presentation

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Title: Bioengineering the Future with Shell Oil Transport Fuels 75


1
Bioengineering the Future with Shell Oil
2
Transport Fuels
  • 75 of liquid fuels are used for transport. 70
    of that in cars.
  • Retail fuel is 95 fossil alkanes and 5 from a
    biological source.
  • By 2020, global demand for bio-derived fuels will
    be 500B litres.

3
The Exeter-Shell Projects
Development of High-throughput Mutant Analysis
and Wax Imaging
Bacterial Population Sampling for HC Production
Alkane Synthesis in Mycobacterium
Inducible Wax Synthesis In Salt Cress
Genes for Alkane Production in Higher Plants
High-throughput Screen Development
Rhodosporidium toruloides Genome Analysis
Alkane Production Secretion in Vibrio furnissii
Forced Evolution Mutagenesis for Increased FA
Gene Pathway Discovery
HC Synthesis Screen in Oleaginous Yeasts
Molecular HC Analysis Of Isolated Mutants
Molecular Bioinformatics
HC Analysis
Molecular Control of HC Production Secretion
in Botryococcus braunii
Molecular Control of Hydrocarbon Synthesis
Secretion in Bacteria, Yeast and Algae for
Advanced Biofuel Production
Botryococcus braunii Culture Systems Productivity
HC Extraction
B. Braunii Genome Evolution
B. braunii-Bacterial Interactions
Alkane Synthesis in Desulfovibrio
Metabolism Enzymes
Culture Systems DSP
Synthetic Ecology for Optimum Algal Growth
Alkane Synthesis in cyanobacteria
Synthetic Biology
C-Flux Engineering In Yeast
FA Catabolism in cyanobacteria
Variable Chain Length Branched HC in
Fermentative Microbes
4
Synthetic Biology
ILV
MDHLA
Keto acid
bkdAA bkdAB
ilvE
bkdB
acoL
  • Engineer the production of gasoline, jet-fuel,
    diesel and lubricant oil in E. coli that are
    identical to fossil fuels.
  • Biological Alkanes require no alteration of
    current engine technologies.

Keto acyl CoA
Malonyl-ACP
Acetyl CoA
accA to accD
fabHB
fabH
Fatty acyl-ACP
Branched fatty acyl-ACP
tesA
luxD
luxD
FFA
FATB1
FATB1
luxC luxE
F-al
NpAD
Alkanes alkenes
5
Third Generation Biofuels from Algae
  • Renewable.
  • Sustainable.
  • Productive.
  • CO2 Mitigation.
  • Experimental.

6
Botryococcus braunii Oils.
  • Synthesises and secretes very long chain
    hydrocarbons.
  • Major fossil component of oil wells.

7
Botryococcus braunii Research.
  • Genome sequencing and bioinformatics to elucidate
    oil biosynthetic pathways interactions with
    symbiotic bacteria.
  • Quantify B. braunii physiology for Life Cycle
    Analysis.
  • Model and test oil production in industrial
    culture conditions.
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