Plus HindIII restriction enzyme

1
5
3
A A G C T T
T T C G A A
3
5
Plus HindIII restriction enzyme
5
3
3
5
C
T T
A
G
A
A
T T C G A
5
3
3
5
Sticky ends
2
Plasmid from a bacterium
DNA of interest from another organism
Both are treated with HindIII restriction enzyme
Mix, allow complementary ends to pair, join cut
ends with DNA ligase
3
Main bacteria DNA
Bacterium
Plasmid
(b)
(c)
0.5 µm
4
Figure 14-13Page 283
5
Figure 14-15Page 285
6
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