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AntiBacterial Properties of Silver Nanoparticles

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Title: AntiBacterial Properties of Silver Nanoparticles


1
Anti-Bacterial Properties of Silver Nanoparticles
2
Objective
  • You will learn about
  • Nanotechnology
  • Making nanoparticles
  • Applications of nanoparticles

3
Motivation
  • Technology involving features with a size between
    1 and 1000 nanometers can be considered
    nanotechnology.

4
Motivation
  • Technology involving features with a size between
    1 and 1000 nanometers can be considered
    nanotechnology.

5
Motivation
  • Nanotechnology is an emerging industry which is
    bringing us exciting new products and promises to
    change the way we live and work in the future.

Nanowire Chemical Sensor
6
Motivation
  • Nanotechnology is an emerging industry which is
    bringing us exciting new products and promises to
    change the way we live and work in the future.

7
Food containers
  • Your food stays fresher longer so you throw away
    less. (How much cash do you throw in the trash?)

http//www.bluemoongoods.com/silver_nanoparticle_f
ood_containers.htm
8
Baby bottles
  • Through silver nano poly system, 99.9 of germs
    are prevented and it maintains anti-bacterial and
    deodorizing functions as well as freshness

http//babydream.en.ec21.com
9
Toothpaste
  • Prevent gum inflammation, periodontitis, and
    periodontal disease
  • Sterilizes and disinfects the Bacilli that is a
    major cause for all gum disease by pure silver
    powder of purity 99.99

http//www.gnsnanogist.com/
10
Toothbrush
  • Songsing nanotechnology company

http//www.ssnano.net/ehtml/detail1.php?productid
73
11
Cuttingboard
  • Antibacterial Effect by Nano Silver Clean

http//www.adox.info/?docshop/item.phpit_id0001
23
12
Computer mouse
  • IOGEAR's Personal Security Mouse is coated with a
    Titanium Dioxide (TiO2) and Silver (Ag)
    nano-particle compound. The coating uses two
    mechanisms to deactivate enzymes and proteins to
    prevent a wide spectrum of bacteria, fungi, and
    algae from surviving on the surface of the
    mouse.

www.iogear.com/main.php?locproductItemGMEFP1W6
13
Antibacterial athletic sock
  • Cushioned, fitted, quarter-length sports socks
    knitted with a cotton material containing
    millions of invisible silver nanoparticles.
  • Innovative socks are naturally antibacterial
    and antifungal

http//www.sharperimagebest.com/zn021.html
14
Cotton sheets
  • Whether you are looking for health benefits, the
    feeling of extra freshness or just the feeling of
    100 cotton sheets, AgActive sheet sets are the
    definition of luxury and can also help in the
    fight against cross infection of super bugs such
    as MRSA.

http//www.agactive.co.uk/index.cfm/fuseaction/pro
duct.display/Product_ID/8/.htm
15
Washing machine
  • Globally recognized for its high-tech and
    futuristic appliances, Samsung incorporates a
    technology called Silver Nano. C1235A 5.2 kg Drum
    Type washing machine comes with Silver Wash that
    sterilizes your clothes. Plus with its stainless
    steel tub, it can help you save water up to 30

http//www.samsung.com/ph/products/washingmachine/
drum/c1235a.asp?pageSpecifications
16
Today's activities
  • Make nanoparticles
  • Soak filter in nanoparticles
  • Inoculate plate
  • Check results

17
How to make silver nanoparticles
  • Put 2 ml .5 mM AgNO3 in a test tube
  • Start with a compound that has silver in it
  • Our solution is silver nitrate

18
How to make silver nanoparticles
  • Put 2 ml .5 mM AgNO3 in a test tube
  • Heat in boiling water bath for 5 minutes
  • Heating it will speed the reaction

19
How to make silver nanoparticles
  • Put 2 ml .5 mM AgNO3 in a test tube
  • Heat in boiling water bath for 5 minutes
  • Add 5 drops of 1 sodium citrate
  • Carefully add the sodium citrate,
  • the solution is HOT
  • Allows gold to form stable nanoparticles

20
How to make silver nanoparticles
  • Put 2 ml .5 mM AgNO3 in a test tube
  • Heat in boiling water bath for 5 minutes
  • Add 5 drops of 1 sodium citrate
  • Continue heating wait for silver nanoparticles
    to form.
  • Watch for a change in color to indicate the
    silver has formed
  • Let it heat a few more minutes to be sure the
    color change is complete

21
How to make silver nanoparticles
  • Put 2 ml .5 mM AgNO3 in a test tube
  • Heat in boiling water bath for 5 minutes
  • Add 5 drops of 1 sodium citrate
  • Continue heating wait for silver nanoparticles
    to form

22
Growth of bacteria
  • Bacteria may grow as a group colony
  • Bacteria may cover surface of plate lawn

23
Bacterial antibiotic sensitivity
  • Antibiotics may inhibit the growth of some
    bacteria
  • Evidence of this is a halo
  • A halo indicates a zone where bacteria are not
    present

24
Procedure
  • Cut filter paper into small squares
  • Place squares in a petri dish and pour silver
    nanoparticles over them
  • Let the squares soak for about 10 minutes

25
Procedure
  • Mark the bottom of an agar plate with your
    initials, divide the bottom into sections, and
    label
  • Put 1 to 2 drops of bacterial culture on the
    plate
  • Spread the drops

26
Procedure
  • Place your nanoparticle soaked filter paper
    squares and your control(s) in the designated
    areas
  • Incubate your plate for 24 hours at 37ÂşC

27
Questions
28
END
  • The following slides display results and are
    included only as a teacher resource for a
    discussion after the experiment has been
    completed.

29
Results
30
Results
31
Results
32
Results
33
Results
34
Results
35
Results
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