Molecular Typing of Salmonella enterica Serovars using Two PFGE Systems - PowerPoint PPT Presentation

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Molecular Typing of Salmonella enterica Serovars using Two PFGE Systems

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Martin Cormican. Interim Salmonella Reference Laboratory. Ireland. Serovars ... Time Pharmacia 6 Days. CHEF Mapper 4 Days (3) Reproducibility Both reproducible ... – PowerPoint PPT presentation

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Title: Molecular Typing of Salmonella enterica Serovars using Two PFGE Systems


1
Molecular Typing of Salmonella enterica Serovars
using Two PFGE Systems
Geraldine Doran Martin Cormican
Interim Salmonella Reference Laboratory Ireland
2
Molecular Typing of S.enterica Serovars using
two PFGE Systems
  • Serovars

S. Kedougou S. Bredeney
  • Systems Pharmacia Gene Navigator
  • Bio-Rad CHEF Mapper (PulseNet)

3
S. Kedougou Results
  • Pharmacia 4 Subtypes
  • CHEF Mapper 7 Subtypes

4
Molecular Typing of S.enterica Serovars using
two PFGE Systems
Comparison of 2 Systems
  • Time Pharmacia 6 Days
  • CHEF Mapper 4 Days (3)
  • Reproducibility Both reproducible
  • Result Quality CHEF Mapper Easier to intrepret
  • Standardised

5
PFGE of S. Kedougou using Pharmacia
? A1 A1 A2 ? A3 A3 A3 A4
? ? A4 A4 A1 ?
kb
339
291
242
194
145
97.0
48.5
6
PFGE of S. Kedougou using CHEF Mapper
? A1 A6 A2 ?
A3 A3 A5 A4 ? A5
A5 A7 A1 ?
kb
339
291
242
194
145
97
48
7
PFGE of S. Kedougou
CHEF Mapper Pharmacia
kb
? A1 A1 A2 ? A3 A3 A3 A4 ? A4 A4 A4
A1 ?
? A1 A1 A2 ? A3 A3 A3 A4 ? A4 A4 A4
A1 ?
339
242
145
97
48
8
S Kedougou Pharmacia Reproducibility
Run 1
Run 2
? A1 A1 A2 ? A3 A3 A3 A4 ? ? A4 A4
A1 ? ? A1 A1 A2 ? A3
A3 A3 A4 ? A4 A4 A4 A1 ?
kb
339
291
242
194
145
97
48
9
PFGE of S. Bredeney
CHEF Mapper
Pharmacia
? A A2 A3 ? B C D E ?
F G
? A A2 A3 B C D
E F G ?
kb

291
242
194
145
97
48
10
Dendogram of S. Kedougou using Bionumerics
Software
11
Conclusions
CHEF Mapper Quicker Easier to
Intrepret More Reproducible Standardised
(PulseNet)
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