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CBoL Taiwan 2007

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Title: CBoL Taiwan 2007


1
CBoL Taiwan 2007
African Wildlife Tissue Banking Programme
Paul Bartels Head, wBRC / BioBankSA National
Zoological Gardens of SA National Research
Foundation
2
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3
Barcode Role Players
  • In southern Africa..
  • SAIAB (NRF) - Fish
  • NZG / BioBankSA (NRF)
  • SANBI
  • UJ - Plants

4
Contents
  • Wildlife Tissue Sampling / Banking In SA
  • Field Sampling / Challenges
  • Multi-Disciplinary Use
  • Plans
  • Aim Appropriately preserved tissue collections,
    collected over time and space

5
  • wBRC BioBankSA - major role in wildlife
    biomaterial collection banking SAs genetic
    resources (National Asset)
  • Resulted in DST/NRF carrying out a due diligence
    process with the aim of transferring the wBRC /
    BioBankSA to the NZG of the NRF
  • 2005 wBRC Transfer to NRF/NZG

6
Biological Resource Bank
  • Biomaterials Blood, skin, hair, sperm, ovaries,
    egg-shells, feathers, cell cultures, organs and
    tissue. (Multi Tissue Types)
  • Biological Resource Bank (BRB) Collection,
    processing, banking use and distribution of
    wildlife biomaterials for Biodiversity
    Conservation and Biotechnology Development

7
South Africa
  • 3rd most biodiverse country in the world
  • plant, bird and freshwater fish diversity, 6 of
    mammal and reptile diversity
  • Smaller organisms????
  • Biodiversity assessment shows continuing downward
    trends.threatened are 14 of birds, 15 of
    plants, 18 of amphibians, 24 of reptiles, 37
    of mammals and climbing!

8
Platform
  • Archival and,
  • Of Use Now Future
  • Multi-Disciplinary Use
  • Endangered spp vs Common spp
  • Focus Knowledge-Hub

9
Platform Spectrum
Biodiversity Biotechnology
10
Multi-disciplinary Uses
  • Disease / Epidemiology
  • Molecular Genetics / Forensics / BoL
  • Reproduction
  • Physiology, Proteomics etc.
  • Specimen Types / Banking Methods

11
Multi-disciplinary Use
  • Specimen Types / Banking Methods
  • Anchor Linked to a Sampling Event
  • (many opportunities out there)
  • Select tissue types
  • Bank in variety of ways
  • Decision-making Process

12
Emerging Infectious Diseases
Human encroachment Ex situ contact Ecological
manipulation
Translocation
Encroachment Introduction Spill over Spill
back
Wildlife
Global travel Urbanization Biomedical manipulation
Domestic Animal
Human
Agricultural Intensification
Technology and Industry
Dasazak P. et.al. Science 2000 287443
13
.

First wildlife calf in Africa born by way of
Artificial Insemination - 1998
14
Role of Zoos
  • Only recently that zoos world-wide have adopted
    conservation of biodiversity as one of their core
    functions
  • Position NZG as a National Research Facility
    within the National System of Innovation

15
Banked Specimens
  • Mammals
  • Cetacea
  • Artiodactyla
  • Perissodactyla
  • Carnivora
  • Primata
  • Proboscidea
  • Birds, Reptiles
  • 450 Species

16
BIOMATERIALBank
  • BRC bank Fibroblast cultures, Biopsies, Gametes,
    Embryos - frozen in LN.
  • Tissue bank skin, muscle, fat etc - frozen, in
    DMSO/Salt, Alcohol
  • DNA bank - Frozen
  • Hair, feathers, bones - Room temp.
  • Pathology Specimen Bank

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21
Web
  • Catalogue
  • SOPs
  • Protocols
  • Special Requests
  • Nodes
  • Contacts

22
Monitoring
  • Staff safety
  • Sample safety

23
Ownership / ABS
  • National, Regional and Global Concern
  • CBD
  • Workshops 2002 / 2006
  • Legislation / Regulations Provincial Regulations
    / Biodiversity Act in RSA / Private Ownership

24
Field Work
25
Constraints, but.
26
Disease survey / Sampling
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.
Semen collection
  • .

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Roan samples from Zambia, 1996
33
Spatial and Temporal..
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Catching them young
37
Technology Transfer
38
Education Training
39
Facilitate collecting
  • Customized sampling kits
  • Quick Reference sheet
  • Field sampling manual
  • Training manual
  • Training courses

40
Animal Species Richness Species of Special
Concern
Birds - 94
Mammals - 79
Frogs - 21
Scorpions - 42
Butterflies - 111
Scarabs - 125
41
Indian sub-continent
42
Southern Africa
43
End Goal
  • SAMPLING EVENT..
  • Biomaterial Banking
  • Other uses
  • Future uses
  • Access Benefit Sharing

44
End Goal cont..
  • PLATFORM..
  • Education Training
  • Policy, Legislation, Regulations
  • HR Development
  • Technology Transfer
  • Infrastructure Development

45
Urgency
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47
BoL SOP
The Mitochondrial Genome
Small ribosomal RNA
  • BoL SOP
  • Contribute to broader bar-coding
  • QC for collection Cell cultures, gametes,
    embryos, hair, egg-shells
  • Parasite / Disease vector ID

D-Loop
ND1
ND6
ND5
COI
ND2
COI
L-strand
H-strand
ND4
ND4L
COII
ND3
COIII
ATPase subunit 8
ATPase subunit 6
48
Logistics
  • In southern Africa.
  • Node development (eg KNP)
  • SADC
  • NEPAD
  • Five Gene-bank Hubs for Africa

49
Conclusion
  • In southern Africa.
  • Platform / Knowledge-hub
  • Biomaterial Bank (reference / future use / LTER)
  • Many Sampling Opportunities out there (network)
  • Opportunity to popularize (involve community)
  • Its wider impact on science, technology,
    biodiversity, Regulatory Issues, MoUs, Research
    MTA
  • Biotechnology Revolution, WTO, ABS Regimes, Prior
    Informed Consent, Regulatory Issues, MoUs,
    CRADAs.

50
Take Along..
  • Biomaterial Bank, Multi-disciplinary
  • Participation by all, Popularize science
  • Barcoding Banking
  • (Catalyst)

51
Take some shoving
52
A New Dawn
  • CBoL
  • NZG/NRF
  • DST
  • NECSA
  • NBN
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