PLACIU GALIMYBIU FEMTOSEKUNDINIS LAZERINIS KOMPLEKSAS MOKSLO, AUKSTUJU TECHNOLOGIJU IR KRATO APSAUGO

1
ROLE and EXPERIENCE of LITHUANIAN REPRESENTATIVES
at ETP
Ricardas Rotomskis Lithuanian Representative at
European Technology Platform Nanomedicine
Biophotonics group Vilnius University Laser
Research Center Sauletekio ale.9, c.III 10222
Vilnius, Lithuania E-mail ricardas.rotomskis_at_ff.v
u.lt http//www.biofotonika.ff.vu.lt/biophotonics
2
Role of Lithuanian representatives at ETP
Disseminate information about Activities, Common
Vision, Strategic Research Agenda, of ETP and
coordinate the National Technological Platform
activities according the common vision and plans
of ETP on the basis of the national strategy and
possibilities
National representatives at the Mirror Group
3

• The ETP Platform Nanomedine- today
• Industry-led consortium of stakeholders
• 160 member organizations
• Industry
• Academia
• Hospitals
• Associations

Members of Lithuanian TP Nanomedicine
Hospitals Vilnius University Santariskiu Clinic
Research institutions Institute of Biochemistry
Institute of Mathematics and Informatics
National representative at the
Nanomedicine Mirror Group
Industry Dialogas, Valentis Sanitas,
Altechna Inovaciju vadyba, Energenas,
Vildoma Imunolita, Grida LAB Optomeda, Medelkom
Universities Kaunas University of
Technology Vilnius Gediminas Technical
University Kaunas Medical university Institute of
Cardiology Vilnius University Institute of
Immunology, Institute of Oncology, Institute of
Experimental and Clinical medicine
4
Role of Lithuanian representatives at ETP
Industry
Sanitas, Valentis pharmaceutical, producers of
drugs, food additives and vitamins
Dialogas, Inovaciju vadyba, management,
information dissemination,
Grida LAB, Vildoma chemistry, microbiology as
well as physical measurements and analytical
equipment
Imunolita, stem cells, cells therapy
Optomeda, Medelkom, Altechna, Energenas,
biomedical optics, microscopy, spectroscopy,
lasers, light emitted diodes
5
LIETUVOS NACIONALINE TECHNOLOGIJU PLATFORMA
NANOMEDICINA     inovatyvios biomedicinines
nanotechnologijos sveikatos apsaugai     Lietuvos
nacionalines platformos (NTP) NanoMedicinos
srityje vizijos metmenys   Vilnius 2006 Liepa
Vision Paper
National representative at the
Nanomedicine Mirror Group
Ministry of Economy
6
Role of Lithuanian representatives at ETP
National representatives at the Mirror Group
Common vission
7
Role of Lithuanian representatives at ETP
To create National Research Strategy on the basis
of the European Strategic Research Agenda
implementing National resources and needs to
European context
8
Strategic Research Agenda of Nanomedicine
LIETUVOS NACIONALINE TECHNOLOGIJU PLATFORMA
NANOMEDICINA   Inovatyvios biomedicines
nanotechnologijos sveikatos apsaugai    
 
9
NTP Nanomedicine
Synthesis and Optical properties of quantum dots
10
NTP Nanomedicine
Au nanoparticles synthesis and optical properties
11
NTP Nanomedicine
Accumulation of quantm dots in cells

• Quantum dots tracking in live cell by confocal
  microscopy

Rabbit stem cells
12
NTP Nanomedicine
Quantum dots
Application of fluorescing nanoparticles
(quantum dots) covered with bioselective coatings
for selective diagnostics of tumours at primary
stages,
ETP Nanomedicine
BaltMet Mayors Meeting Berlin 10-11 February
2005 RiRo
13
NTP Nanomedicine
Application of bioselective nanoparticles
complexed with phototoxic agents for selective
treatment of tumours. Double selectivity on
tumours can be obtained by using photosensitizers
molecules generating a highly reactive singlet
oxygen under illumination at appropriate spectral
region
ETP Nanomedicine
14
NTP Nanomedicine
Studies of Cell Growth Regulation
Platelet-derived growth factor binds, dimerise
and activates its receptor

• How does the growth factor receptor transmit
  cell growth regulation signal ?

Receptor autophosphorylates specific tyrosines
Phosphotyrosines and surrounded amino acids
serve as specific binding sites for PI3K
(Phosphatidylinositol 3-kinase) and PLC?
(Phospholypase-C-gamma)
PI3K and PLC? transmit independent and
redundant cell growth signals
ETP Nanomedicine
Project M. Valius Institute of Biochemistry,
Vilnius, Lithuania
15
Ministry of Economy
Lithuanian Innovation Centre
Ministry of Education and Science
National representatives at the Mirror Group
24 national technology platforms have been
established
European TP Nanomedicine
National Technological Platforms Center
16
The implementation of innovations in NANOMEDICINE

• Centers of nanomedicine
• Web based information dissemination on
  Nanomedicine
• Proposals for 7 th farmework programme
• Proposals for National priorities in advanced
  technology
• Proposals for State science and studies fund

17

• Under agreement among
• Kaunas Medical University and Vilnius University
  (two universities in Lithuania which perform the
  university level medical studies),
• the two biggest clinics in Lithuania Vilnius
  university Santariskiu Clinics and Kaunas Medical
  University Clinics
• and also Institutes of these universities
• the Institute of Oncology,
• the Institute of Experimental and Clinical
  Medicine,
• the Institute of Immunology,
• the Institute of Biomedicine)
• in collaboration with State Center of Pathology
  the
• Biomedical Physics and Nanophotonics Center
  (BioMedPhysNanophotonics Center) is going to be
  established.

18
Ministry of Education and Science
Center is planned as interdisciplinary research
organisation for collaboration between nine
institutions of Medical Research and Studies
including two biggest Clinics of Lithuania and
State Center of Pathology focusing especially on
the implementation of innovations in NANOMEDICINE
19
BioMedPhysNanophotonics Center
The staff of the Center will comprise more than
30 professionals working in the fields of
Physical and Life sciences and Medicine. A strong
collaboration has been established with the
departments of Colloids Chemistry and Polymer
Chemistry of Vilnius University Faculty of
Chemistry with the aim of producing the metal
nanoparticles, quantum dots and other
nanoparticles of different type.
20
Project Kvantameda
Nanoparticles synthesis, modification and
applications
Vilnius University Biophotonics group of Laser
Research Center
Vilnius University Institute of Oncology
Laboratory of Biomedical physics
Vilnius University Department of General and
Inorganic Chemistry
Vilnius University Department of Analytical and
Environmental Chemistry
Institute of Immunology of Vilnius University
Laboratory of Immunoanalysis and Nanotechnology
21
Project NanoCells
Studies of Cell Growth Regulation

• Quantum dots tracking in live cell by confocal
  microscopy

Rabbit stem cells
Project of M. Valius Institute of Biochemistry,
Vilnius, Lithuania
22
Project Kvantameda
Photomodification of (CdSe)ZnS quantum dots
Fig 1. A. Photoluminescence spectra of (CdSe)ZnS
QDs (0.08mg/ml) after UV/VIS irradiation
(1,2,6,12,24,48min.). B Photoluminescence spectra
of mixed (CdSe)ZnS QDs (0.08mg/ml) and pyrrole
(0.05M) solution after UV/VIS irradiation(1,2,6,12
,24 min.).
Fig 2. A. Normalized photoluminescence spectra of
(1) non-irradiated (CdSe)ZnS QDs (0.08mg/ml),
(CdSe)ZnS QDs (0.08mg/ml) after 24 min. UV/VIS
irradiation in absence(2) and presence(3) of
pyrrole (0,05M). B PL intensity kinetics of
UV/VIS irradiated (CdSe)ZnS QDs in absence(1) and
presence(2) of pyrrole.
23
Project NanoImaging
Hydrophobic (CdSe)ZnS quantum dots distribution
in the tissues of experimental animals
24
Project NanoCancer
(CdSe)ZnS quantum dots as photosensitizers
meso-Tetra(4-pyridyl)porphine
Tpyp
3O
1,3 - Diphenylisobenzofuran
1O
25
Programme coordination for efficiency
Nature shows us the way Birds coordinating their
efforts
71 gain of efficiency
26
Chindia
United States
European Union United in Diversity
www.scanbalt.org
27
Thank you for your attention
Biophotonics group Laser Research Center
Vilnius University Sauletekio ale.9, c.III 2040
Vilnius, Lithuania E-mail ricardas.rotomskis_at_ff.v
u.lt
Ricardas Rotomskis
28
Project NanoCells
Studies of Cell Growth Regulation

• Discovery of cancer cell surface biomarkers for
  diagnostics and therapy prognosis

Mass spectrometric protein identifications iTRAQ
FOF/TOF mass spectrometry ABI 4800
Membrane protein separation 2D-Electorophoresis
affinity and 2D-chromatography
Samples from clinical biopsy materials, cancer
cell lines, etc.
Increase clinical samples throughput for
biomarker validation
Mass spectrometric protein identifications Multipl
e Reaction Monitoring (MRM) ABI 4000 QTRAP
Computation analysis Data base search, data mining
Cell surface BIOMARKERS for cancer cell
visualization and targeting
Project M. Valius Institute of Biochemistry,
Vilnius, Lithuania
29

• For coordination we need
• Selection of National priorities
• Financial support and interest from government
• Advisory board

30
Project NanoCells
Studies of Cell Growth Regulation

• Nanopartical-coupled PDGF for cancer cell
  tracking and targeting

Growth signaling, tracking, targeting
Synthesis of recombinant wild type PDGF dimmer
NO Signaling, tracking, targeting
Synthesis of recombinant PDGF monomer
Project M. Valius Institute of Biochemistry,
Vilnius, Lithuania