DNA St. Patricks Day Salute to the Molecule of Heredity From Biology 110 UNC 1993 Steve Lee - PowerPoint PPT Presentation

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DNA St. Patricks Day Salute to the Molecule of Heredity From Biology 110 UNC 1993 Steve Lee

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DNA St. Patricks Day Salute to the Molecule of Heredity. From Biology 110- UNC 1993 Steve Lee ... DNA strands are just not the same. One is coding and one is ... – PowerPoint PPT presentation

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Title: DNA St. Patricks Day Salute to the Molecule of Heredity From Biology 110 UNC 1993 Steve Lee


1
DNA St. Patricks Day Salute to the Molecule of
HeredityFrom Biology 110- UNC 1993 Steve Lee
  • The molecular structure today
  • Is hereditys DNA
  • With nucleotides
  • completely comprised
  • of a sugar and phosphate and base
  • The bases you see are so keen
  • They include thymine and adenine
  • Cytosine and one more
  • with guanine can store
  • all the info with rungs in between
  • The sides of the ladder you know,
  • are sugar and phosphate which show
  • that Franklin was right
  • double helix is tight
  • ten base pairs per turn in a row

Adenine and thymine can base pair Forming two
hydrogen bonds for one stair Cytosine and
guanine pair with three in between and are equal
in size when compared DNA strands are just not
the same One is coding and one is called lame
(anticoding) They are opposite in direction and
this is called antiparallel in name Complimentary
nature of strands lets replication proceed just
as planned with A paring to T and G pairing to
C the fidelity is precise and quite grand
2
Steps in Forensic DNA typing (Figure 6.1 Rudin
and Inman 2001)
Evaluation- Is it there? 1. Start with
biological sample 2. Screen- blood? Semen?
Saliva, human? Extraction- Get and clean DNA 3.
Open cells ? Get DNA 4. Methods to get DNA
and purify DNA Quantify- Determine quality and
quantity? 5. Quantify- How good and how
much did you get? Type to determine and compare
alleles 6. RFLP vs PCR 7. Determine alleles and
compare DNA types Or alleles present in samples
and references Interpretation of Results
3
Review DNA is organized inside the cell nucleus
and mitochondria
4
DNA Extraction After screening tests are
performed, a spot of the material containing the
biological sample is cut and placed into a
tube. In one type of extraction method
(organic), heat and chemicals are added, and
protein is removed. Then the pure DNA is
recovered by filtration in which the non-DNA
material goes through a sieve. (analogous to a
collection of your pasta in a colander)
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