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Project Idea: Biofuels

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Project Idea: Biofuels. Stanford iGEM 2009. Presentation by: Suzie. Robert. 3/4/09 ... We would need to engineer a separate amino acid pathway to produce a ... – PowerPoint PPT presentation

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Title: Project Idea: Biofuels


1
Project Idea Biofuels
  • Stanford iGEM 2009
  • Presentation by
  • Suzie
  • Robert
  • 3/4/09

2
Biofuels Introduction
  • What makes a good biofuel?!?!
  • Environmentally friendly
  • Mass production - CHEAP!
  • Chemically
  • High energy density
  • Low water solubility

3
Biofuels Introduction
4
Biofuels Introduction
  • As for synthetic biology applications
  • Most use E. coli or S. cerevisiae as a host to
  • 1. Engineer amino acid biosynthesis
  • 2. Engineer fatty acid pathways
  • 3. Engineer isoprenoid pathways

5
Dr. James Liao
6
Dr. James Liao
7
3-Methyl-1-Butanol
8
2-Methyl-1-Butanol
9
Dr. James Liao
10
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11
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12
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13
Our Project
  • We would need to engineer a separate amino acid
    pathway to produce a possible biofuel
  • EX Serine or alanine
  • Need to ID enzymes that can make this possible
  • For Dr. Liao, it was KIVD (yeast) and ADH6
    (lactococcus lactis)

14
Five Possible Sub-projects
  • 1. Synthesizers (constructing the pathway)
  • 2. Riboregulators (controlling knockouts)
  • 3. Modelers (Modeling for bottlenecks)
  • 4. Bottleneckers (Building expression system)
  • 5. ANY OF THE ABOVE

15
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