Tryptophan Synthase in Chlamydia - PowerPoint PPT Presentation

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Tryptophan Synthase in Chlamydia

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Immune response of humans to Chlamydia infection involves the release of interferon. ... Completed Chlamydia trachomatis D/UW-3/CX proteins; ... – PowerPoint PPT presentation

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Title: Tryptophan Synthase in Chlamydia


1
Tryptophan Synthase in Chlamydia
  • Angela Ghrist
  • Lori Scott

2
Background
  • Intracellular parasites viruses, bacteria
    (Chlamydias, Rickettsias), and protozoa
    (plasmodia) (CDC website)
  • Tryptophan biosynthesis genes are found to
    varying degrees within the Chlamydiaceae family
    (Kegg pathway program)
  • Immune response of humans to Chlamydia infection
    involves the release of interferon. This
    activates an enzyme that degrades tryptophan,
    thereby reducing Chlamydia reproduction inside
    the host cell (PubMed)
  • Tryptophan is an essential amino acid in humans

3
(No Transcript)
4
Taxonomy
  • Lineage (full) root cellular organisms
    Bacteria Chlamydiae/Verrucomicrobia group
    Chlamydiae Chlamydiae (class) Chlamydiales
  • Chlamydiaceae
  • Candidatus Clavochlamydia
  • Candidatus Clavochlamydia salmonicola
  • Chlamydia
  • Chlamydia muridarum
  • Chlamydia suis
  • Chlamydia trachomatis
  • Chlamydophila
  • Chlamydophila abortus
  • Chlamydophila caviae
  • Chlamydophila felis
  • Chlamydophila pecorum
  • Chlamydophila pneumoniae
  • Chlamydophila psittaci
  • TaxBrowser in NCBI

5
Available Genomes
  • Completed Candidatus Protochlamydia amoebophila
    UWE25 proteins
  • Completed Chlamydia muridarum Nigg proteins
  • Completed Chlamydia trachomatis A/HAR-13
    proteins
  • Completed Chlamydia trachomatis D/UW-3/CX
    proteins
  • Completed Chlamydophila abortus S26/3 proteins
  • Completed Chlamydophila caviae GPIC proteins
  • Completed Chlamydophila felis Fe/C-56 proteins
  • Completed Chlamydophila pneumoniae AR39 proteins
  • Completed Chlamydophila pneumoniae CWL029
    proteins
  • Completed Chlamydophila pneumoniae J138 proteins
  • Completed Chlamydophila pneumoniae TW-183
    proteins
  • NCBI Genomic Biology

6
Enolase
NCBI - Genome Blast Search and Tree Building
7
DendrogramEnolase
Unrooted tree (generated by Phylip's Drawtree)
Workbench, ClustalW
8
  • Observation
  • There are multiple serovars of Chlamydia
    tachomatis, distinguished by route of infection.
  • Question
  • Are there differences in their trp genes?

9
Comparison of Ocular (A) and Genital (D) TrpA
Genes
  • trpA_D CTTCTACAAAGGGACTTAGATTATCTACGCAGACTA
    AAAGACGCGGGAATAAATGGTGTG
  • trpA_A CTTCTACAAAGGGACTTAGATTATCTACGCAGACTA
    AAAGACGCGGGAATAAATGGTGTG
  • trpA_D TGCGTTATAGATCTTCCAGCACCTTTATCACACGGA
    GAAAAATCTCCATTTTTTGAAGAT
  • trpA_A TGCGTTATAGATCTTCCAGCACCTTTATCACACGGA
    GAAAAATCTCC---TTTTGAAGAT
  • trpA_D CTTTTAGCTGTAGGATTGGATCCTATTTTGCTTATT
    TCTGCAGGGACAACGCCGGAGCGG
  • trpA_A CTTTTAGCTGTAGGATTGGATCCTATTTTGCTTATT
    TCTGCAGGGACAACGCCGGAGCGG
  • trpA_D ATGTCTTTAATACAAGAATACGCAAGAGGCTTTCTG
    TATTATATCCCATGTCAAGCTACG
  • trpA_A ATGTCTTTAATACAAGAACACGCAAGAGGCCTTCTG
    TATTATATCCCATA-CAAGCTACG

10
Ocular vs. Genital Tryptophan Synthase
Polymorphisms in Chlamydia trachomatis tryptophan
synthase genes differentiate between genital and
ocular isolatesJ. Clin. Invest. Harlan D.
Caldwell, et al. 1111757 doi10.1172/JCI17993
11
  • Question
  • Has the Chlamydia L serovar that causes a
    systemic lymph node infection retained the
    tryptophan synthase (trpA) gene like the genital
    serovars, as opposed to acquiring nonsense
    mutations like the ocular serovars?
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