Collaborative Casebased Study of Genetic and Infectious Diseases via Molecular Biology Computer Simu

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Collaborative Case-based Study of Genetic and
Infectious Diseases via Molecular Biology
Computer Simulations and Internet Conferencing

• Mark Bergland and Karen Klyczek, University of
  Wisconsin-River Falls

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Overview

• Description of Case It! Project
• Sample case studies AIDS, STDs
• Online conferencing system / web editor
• Demonstration of software
• ELISA, Western blot, multiplex PCR
• Discussion
• How to use Case It software with students
  (lecture/lab)
• Assessment issues
• Future directions malaria, dengue, other
  infectious diseases of worldwide importance

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Case It! Project

• URL for Case It! Home Page
• http//caseit.uwrf.edu (on handout)
• Includes tutorials and download links
• Powerpoint will be available from above URL
• Contact mark.s.bergland_at_uwrf.edu
• Supported by the CCLI program of the
  National Science Foundation

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Case It! Project

• Electronic framework for analyzing and discussing
  case studies in molecular biology
• Genetic and infectious diseases and associated
  ethical issues
• Students gather background information on cases
• Analyze DNA and/or protein sequences using
• Case It! Version 5.02 beta (5.03 to be released
  soon)
• Prepare web-page poster using automated system
• Poster session via Internet conferencing
• Role-playing

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Techniques for DNA and protein analysis

• Features of Case It! simulation
• DNA and protein electrophoresis
• Restriction enzyme digestion and mapping
• Southern blotting
• Dot blotting
• Polymerase Chain Reaction (single and 96-well)
• ELISA
• Western blotting

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Case It! web editor

• Features of the Case It! web editor
• Web page construction
• Conferencing/discussion board
• Collaborate with classes at other institutions
• Instructors can add classes
• Upload class roster, organize teams

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Case analysis

• Case It! Investigator is used to gather
  background information on case
• Case It! Version 5.0 beta is used to analyze DNA
  sequences associated with case
• Case It! Launch Pad gives access to a web
  editor/conferencing system
• -electronic poster sessions
• -role playing
• Online tutorial gives detailed instructions

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(No Transcript)
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View sample student posters
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HIV Case studies

• Case scenario - video and text , using
  Investigator v2
• ELISA test - initial screening
• Western blot to follow up ELISA results
• PCR to measure viral load
• Upload graphical results to Case It web editor

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ELISA test results
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HIV viral proteins
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Western BlotBand pattern interpretation
Lane 1, HIV serum (positive control) Lane 2,
HIV- serum (negative control) Lane A, Patient A
Lane B, Patient B Lane C, Patient C
Band interpretation
from www.biology.arizona.edu
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HIV Case studies

• Case scenario - video and text , using
  Investigator v2
• ELISA test - initial screening
• Western blot to follow up ELISA results
• PCR to measure viral load
• Upload graphical results to Case It web editor

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STD Case study

• Developed at a recent BioQUEST workshop by C.
  Dinitra White, North Carolina AT
• Demonstrates an additional feature of the
  software (multiplex PCR)
• Software can be adapted for any case scenario

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The Case
The Soldiers Unexpected Gift
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Robert the Young Soldier

• Robert Jr. is a 22 year old soldier who very
  recently returned to his hometown in the
  Midwestern region of the United States
• Lucky for Robert, his assignment in Asia ended
  just in time to return to Old School University
  to complete a degree in Science and Mathematics
  Education.

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The Story Continues

• To celebrate his return, Roberts girlfriend
  Jenna, a foreign exchange student, gathered lots
  of food, alcohol, and party favors for a night of
  celebration with friends.
• Several days later, the director of Old School
  Universitys student health department issued an
  alert to the university president and the local
  department of health to report a dramatic
  increase in the number of GUD cases on campus.

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The Story Continues

• There were lots of rumors about an outbreak of
  HIV or Gonorrhea on campus, however no official
  warnings were released by the university.

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What will they test?

• Samples represents actual DNA sequences from five
  sexually transmittable microorganisms
• Haemophilus ducreyi (Chancroid)
• Herpes Simplex Virus-2 (Herpes)
• Neisseria gonorrheae (Gonorrhea)
• Chlamydia trichomatis serovar D (Trich)
• Treponema pallidum (Syphilis)

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Gel Loading Key

• Positive Control
• College Student 1
• Solider (Robert)
• College Student 2
• Jenna

Common Names
Syphilis
Herpes
Gonorrhea
Trich
Chancroid
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Assessment

• Discussion
• How to use Case It software with students
  (lecture/lab)
• Assessment issues
• Future directions malaria, dengue, other
  infectious diseases of worldwide importance

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Comments from Zimbabwe students

• To me it was an eye opener Since Zimbabwe is
  hard hit by HIV it is important to have this
  project CASE IT since it affects the students
  lives at large. The videos shown are touching.
  These can help students feel it.
• It was also interesting to network with
  international students via conferencing. Indeed,
  the world is a global village.
• (I) was able to get an understanding of how
  American families react to their family members
  when they have tested HIV positive

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Comments from Zimbabwe students

• I had no idea how I could do practicals on H.I.V
  testing given the limited apparatus and financial
  limitations in my school.
• I found the use of the computer very useful in
  simulations because it enables the carrying out
  of experiments which would otherwise be
  impossible due to the costs involved

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Comments from Zimbabwe students

• The programme was also very interesting because
  it had cases of real people that got infected in
  different ways and situations and the
  counselling they were given and how some observed
  the advice and some ignored this advice got
  themselves into worse trouble.

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Comments from Zimbabwe students

• The subject still remains every teachers
  concern. Of particular concern however, is the
  increase in the number of orphans. It is
  estimated that by the year 2005 approximately
  910000 children will have lost their parents to
  AIDS. Poverty, depression, and stigma will haunt
  most of these children. They will most likely
  expose themselves to HIV infections in pursuit of
  basic need and will painfully erode their basic
  right to family, protection health and food. Our
  challenge is therefore to intensify awareness
  education to these children.

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Invitation to participate

• We welcome participation in the Case It! Project
• No cost to educators
• High school and university educators are invited
• Contact mark.s.bergland_at_uwrf.edu