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Title:

Cryogenic Freezing of Dissection Samples

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Regulation of Cryogen Flow, Temperature, and Pressure. Design Overview ... Cryogen Volumes. Length of the Copper Tubing ... to Optimize Cryogen Flow ... – PowerPoint PPT presentation

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Title: Cryogenic Freezing of Dissection Samples


1
Cryogenic Freezing of Dissection Samples
  • Gretchen Foltz, Marie Meyer, Kristy Wood, and
    Jodi Zilinski
  • BME 400
  • October 19th, 2001

2
  • ClientProfessor Barry Ganetzky
  • YoungHo Koh, PhDDepartment of GeneticsUniversity
    of Wisconsin MadisonAdvisorMitch
    TylerDepartment of Biomedical EngineeringDepartm
    ent of Rehabilitative MedicineUniversity of
    Wisconsin - Madison

3
Overview
  • Problem Statement
  • Background
  • Key Specifications
  • Design Overview
  • Components
  • Cooling Mechanism
  • Electrical Control
  • Spray Chamber
  • Final Design Preview
  • Future Aspects
  • Questions

4
Problem Statement
  • To design a device which is able to
  • Rapidly freeze biological specimens
  • Provide maximum vitrification
  • Preserve morphology

5
Background
  • Study of Synapses in Drosophilia
  • Electron Scanning Microscope
  • Requires New Fixation Techniques

6
Key Specifications
  • Preservation of Morphology
  • Rate of Freezing
  • Cost
  • Regulation of Cryogen Flow, Temperature, and
    Pressure

7
Design Overview
Phase I
Phase II
Phase III
Cooling Mechanism
Overall Design
Integration And Testing
Electronic Control
Spray Chamber
8
Cooling Mechanism
  • Primary Cryogen Liquid Nitrogen
  • Secondary Cryogen Propane
  • Component Elements
  • Flow Rates
  • Cryogen Volumes
  • Length of the Copper Tubing

9
Thermo Calculations
  • Amount of heat that must be removed for the
    sample to properly freeze specimen.
  • Amount of liquid propane needed to extract the
    heat in the sample.
  • Amount of liquid nitrogen needed to condense and
    cool the propane.
  • Length of copper tubing.

10
Thermo Calculations
11
Electronic Control
12
Spray Chamber
  • Spray Mechanism
  • Insulating Materials
  • Vacuum Pump
  • Solenoid Valves

13
Final Design Preview
14
Spray Nozzle
Copper Tubing
ID 0.4868 cm OD 0.9970 cm
5 cm
15
Copper Tubing
15 cm
Flask
15 cm
16
Copper Tubing
15 cm
1 cm
Platform
Flask
15 cm
17
(No Transcript)
18
21 cm
15 cm
19
Future Aspects
  • Complete Engineering Drawings
  • Build Prototype
  • Testing
  • Run a Design of Experiment to Optimize Cryogen
    Flow
  • Make Necessary Adjustments to Design for
    Construction of Final Product
  • Write Safety Manual for Operation

20
Questions
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