Title: Figure v' fkh1fkh2 and isogenic wildtype cells show similar responses to HP and osmotic stress' A,B,
1Figure v. fkh1fkh2 and isogenic wildtype cells
show similar responses to HP and osmotic stress.
A,B,D,E, Time course experiments of cell cycle
progression in synchronized cultures of strains
DBY8781 (wildtype A and D) and DBY8834 (fkh1fkh2
double mutants B and E). Cultures synchronized
at G1 were divided into equal volumes, released
from arrest at time zero, and treated with the
designated treatments starting at the time points
indicated by arrows. A, B, HP treatment before
completion of S phase (25 and 38 minutes after
release, respectively following addition, HP
concentration was not monitored). D, E, mild
osmotic stress before completion of S phase (25
minutes after release). Note the lack of S phase
delay in fkh1fkh2 cells, which progressed in the
cell cycle essentially as did wildtype cells. A
moderate osmotic stress (0.7 M NaCl), caused an
immediate growth arrest in both wildtype and
mutant strain cultures (not shown). Shown are
percentages of G1 cells (blue lines), S phase
cells (red lines) and G2/M cells (grey lines) out
of the total number of cells counted. C, Cell
viability under exposure to HP. Exponentially
growing cultures were exposed to 2.4 mM HP for 1
hour (or left untreated), serially diluted (110)
and spotted onto a YPD plate. Strains shown (top
to bottom) are DBY8781 and DBY8834. Note that
fkh1fkh2 cells (strain DBY8834) are not more
sensitive to HP than the isogenic wildtype strain
(DBY8781).