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Visual masking with optical imaging of intrinsic signal, twophoton calcium imaging, and single unit

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Title: Visual masking with optical imaging of intrinsic signal, twophoton calcium imaging, and single unit


1
Visual masking with optical imaging of intrinsic
signal, two-photon calcium imaging, and single
unit recording.
MNTP2009 Optical Imaging Modality
  • Anastasia Gorbunova, MA (University of Arizona)
  • Modality instructor Dr. Justin Crowley
  • Training Assistant Corey Flynn

2
Visual Masking
  • Destructive interaction or interference among
    transient stimuli that are closely coupled in
    space or time

Forster Davis (1984) J Exper Psych Learning,
Memory Language

doctor
DOCTOR
doctor

R. Fox. (1998). In Handbook of Sensory
Physiology. VIII. Perception.
3
Optical Imaging Modality
  • Optical imaging of intrinsic signal
  • - wide spatial field
  • - temporal resolution
  • slow hemodynamic dependent
  • faster light scattering properties of tissue
  • Single unit recordings
  • - best temporal resolution
  • Two-photon Calcium imaging
  • - distinct neurons, fine time course

4
Stimuli
1.
2.
Time
4.
3.
Time
5
Stimuli
5.
6.
7.
8.
6
Intrinsic Signal OI
  • Exposed visual cortex of a ferret (P80-130)
  • Illumination by 700 nm light
  • Visual stimulation (4 degree bars)
  • CCD camera (100 ms data frames)
  • Computer digitization

Courtesy of David E. Whitney
7
Experiment 2 F09-051
1 Block
-

8
Experiment 2 F09-051
8 s
8 s
8 s
100 ms
100 ms
100 ms
9
Experiment 2 F09-051
1 frame 500 ms
10
Extracellular Single Unit Recordings
  • Exposed visual cortex of a ferret (P80-130)
  • Carbon fiber electrode
  • 20kHz signal digitization

11
Extracellular Single Unit Recordings
B
H
H
V
V
B
SQP
SNP
SNP
SQP
H-B
H-B
V-B
H-V
V-H
V-H
H-V
V-B
H, horizontal grating B, blank V, vertical
grating SQP, square-wave plaid SNP, sine-wave
plaid
12
2-photon calcium imaging
  • Oregon green BAPTA-1AM (labels neurons and
    astrocytes)
  • Sulphorhodamine-101 (labels astrocytes)
  • Microscope Zeiss LSM 510 Meta NLO with MIRA900
    Ultrafast TiSapphire laser
  • 20x NA1 objective

13
Calcium imaging
14
2-photon calcium imaging
3 cells ?F/F for 700 frames normalized over 10
blocks
15
Conclusions
  • It is possible to study masking in animal models
    using simple visual stimuli
  • Very brief presentation of static gratings
    produces sufficient activation detectable by
    optical imaging of intrinsic signal
  • Orthogonal mask disrupts that activation

16
Acknowledgements
Dr. Justin Crowley Corey Flynn David
Whitney Sruthi Chintakunta Alberto Vazquez
Dr. Seong-Gi Kim Dr. Bill Eddy Tomika
Cohen Rebecca Clark
Thank you!
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