Overview of Process Tracking

1
Overview of Process Tracking
Sequencing Genomic DNA
BAC Binning
Mutant Generation and screening
cDNA Library Production Arraying Sequencing
2
Tracking System
Objectives

• State of processing
• Location of materials
• History of processing
• Easy utilization
• Flexible

3
Current System

• Sufficiently robust for current operations
• Amalgamation of manual and electronic
• Audit trail
• Allows for verification

4
Current Model
5
Re-implemented System

• Captures current practices
• Provides for increased scale of operations
• Easier generation of tracking reports
• Handles inter-laboratory transfers
• More uniform user interface

6
Re-implemented Model
7
Mutant Phenotype Database

• Experimental Information Capture

8
Utilization

• Information capture
• Source annotation information
• Easy, public Web access
• Data relationships
• Mutation methodology
• Experimental results
• Genetic information

9
Structure
Mutant Phenotype Database
MGOS
10
Mutant Phenotype Database
Mutant Phenotype Processing
Public Web Access
Mutant Phenotype Database
Annotation data
MGOS
NCSU Phytotron
11
An Example of Process Tracking and Data
Acquisition
Ship to NCSU
Single Spore isolation
Transformation
Morphology Analysis
Produce Conidia
Pathogenicity Assay
Generate DNA Pools
Single Spore isolation
Transformation
Morphology Analysis
Ship to NCSU