Method Development for Russian Nanobiosafety System Vladimir Popov A.N.Bach Institute of Biochemistr

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Method Development for Russian Nanobiosafety
SystemVladimir PopovA.N.Bach Institute of
Biochemistry RAS
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Goals and aims

• Scientific and methodological basis of safety and
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  parameters to be controlled and their detection
  ranges (?????????????? ????????? ? ????????? ??
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Support of nanosafety RD activities
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Nanosafety projects of the Federal Program
Development of Nanoindustry Infrastructure
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Method development projects of the Federal
Program

• Development of methodology and elaboration of
  tools for sampling, identification and detecion
  of effects of technogenic nanoparticles in living
  organisms
• Development of methodology and elaboration of
  tools for control of nanoparticles localization
  in organs and tissues
• Development of methodology and elaboration of
  tools for evaluation of effects of nanomaterials
  on heredity system
• Development of methodology and elaboration of
  tools for laboratory in vitro testing of
  biological activity of nanomaterials
• Development of methodology of biotesting and
  elaboration of tools for detection of
  nanoparticles in the environment for assessment
  of their safety, distribution, accumulation and
  effect

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Sectoral projects and Integrated project of the
Federal Program

• Development of regulations and tools for control
  of nanoparticles at industrial premises
• Development of regulations and tools for control
  of nanoparticles content in agricultural
  production, foodstuffs and packaging
• Development of regulations and tools for control
  of nanoparticles content in household chemicals,
  perfumes and cosmetics
• Integrated project
• Creation of the integrated system to provide
  safety in the course of RD, manufacturing,
  application, circulation and utilization of
  nanomaterials in the Russian Federation

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Characteristics of NP important for biological
safety issues

• Size size distribution
• Surface area
• Charge
• Shape
• Chemical composition
• Particle number
• Structure
• Solubility
• Impurities
• Chemistry reactivity

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Detection of NPs choice of method

• Applicability to real multi-component samples
• Sample preparation should not perverse data on
  particle size and aggregation state
• Relatively simple methods to exclude artifacts

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Particle characterization
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Special features of the analytical methods for NP
control
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Difficulties in NP assays

• Detection of NPs in biological matrices
• Currently not sufficient
• Reference materials
• There is a lack of availability
• Protocols and standardization
• A large batch to batch and production variability
  of commercially available ENPs
• General toxicity tests
• Uncertainty on sensitivity of existing (OECD)
  tests methods / protocols for the assessment of
  ENPs effects
• Mutagenicity, carcinogenicity, neurotoxicity and
  immunotoxicity
• These are of high concern and difficult to asses
  in current systems
• Environmental fate and effects
• Currently not fully possible to estimate in
  practical terms
• Metrics (dose response relations)
• Currently there is no available procedure to
  correlate chemico-physical properties of ENPs
  with observed biological interactions

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NP fate in the environment
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NP in the environment
Exposure predictions
A. Boxall, 2008

• Very low concentrations have to be detected
• Sample preparation technique should preserve
  data on the aggregation state

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Criteria for NP selection
Priority candidate materials for nanotoxicology
reference materials
Carbon blackA, B, C
ZnOA, B
AgA
TiO2A, B, C
Polystyrene (fluorescent) B
Carbon nanotubesA, B
Aitken et al., 2008
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NPs OECD priority list

• Titanium oxide
• Aluminium oxide
• Cerium oxide
• Zinc oxide
• Silicon dioxide
• Polysterene
• Dendrimers
• Nanoclays

• Fullerenes
• Single-walled carbon nanotubes (SWCNTs)
• Multi-walled carbon nanotubes (MWCNTs)
• Silver nanoparticles
• Iron nanoparticles
• Carbon black

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Program coordination

• Bank of standard NPs
• Standard calibration method(s)
• Standard protocols
• Scientific Council responsible for method
  standardization between Federal Program
  participants

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Thank you for attention!
Colloidal gold nanoparticles
Axes 37.27.9 x 29.65.2 nm
Axes 25.75.8 x 21.03.2 nm
Axes 13.31.9 x 11.91.4 nm
Dynamic light scattering
Electron microscopy data
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ENPs fate in the environment
Oberdorster et al., 2005