Field%20Performance%20of%20Double%20Haploid%20Dill%20(Anethum%20graveolens)

1
Field Performance of Double Haploid Dill (Anethum
graveolens)

• Doug Waterer
• and Alison Ferrie

2
Double Haploids ?

• In some species it is possible to generate
  haploid plantlets usually from microspores.
• Diploid produced by doubling (colchicine)
  chromosomes in haploid

3
Microspore culture
Colchicine treatment
4
Why Double Haploids ?

• Advantages
• Produces homozygosity without extensive selfing
  time saving
• Fixes recessive traits useful
  genetics/breeding tool
• Disadvantages
• Unmasks recessive traits
• Loss of heterosis Inbreeding depression ?

5
(No Transcript)
6
Double Haploids

• Projective Objectives
• - develop protocols for creating double haploids
  of economically significant Apiaceae
• - evaluate field performance of DH lines for
  potential commercial release or for use in
  breeding programs

7
(No Transcript)
8
Embryo and Plant production
Donor plant conditions Genotype Developmental
stage Pretreatments Media composition Culture
environment
Genotype Embryo stage Pretreatments Media
composition Culture environment
9
Apiaceae species in DH Program

• Ajowan
• Angelica
• Anise
• Bupleurum
• Caraway
• Carrot
• Celery
• Chervil
• Coriander
• Cumin

• Dill
• Dong quai
• Fennel
• Gota kola
• Laceflower
• Lovage
• Mitsuba
• Parsley
• Parsnip
• Samphire

10
Field Trials of DH Lines

• 2002-2006
• Assess field performance of DH lines of Dill and
  other Apiaceae
• Looking for differences from parental lines
• Evidence of inbreeding depression ?
• Field trials conducted in Saskatoon, SK

11
Field Trials of DH Lines

• Significant portion of the DH lines fail to
  germinate homozygous trait is fatal

12
Field Trials of DH Lines

• Other DH lines show reduced seedling vigor versus
  parent
• Some DH lines have superior germination
  characteristics compared to parental lines

13
Plant Development

• Significant variation in development of some DH
  lines versus parental lines

14
Plant Development
/- 16 cm

• DH lines show much greater uniformity of height
  than parents

/- 4 cm
15
DillDH 12 and Parental
16
Yields

• Significant increase in seed yield possible in
  large part due to more synchronous seed maturity
  less shattering

17

• DH-12
• Flowering July 20

18
Seed Oils

• Some increase in seed oil in early maturing DH
  lines
• Environment effects on seed oil content and
  composition gtgt genotypic variation in DH lines

19
Oil Yield

• Significant increase in oil production/unit area
  largely a function of increased seed yields

20
Field Performance of Double Haploids

• Conclusions
• - DH lines show variability (/-) from parents
• - DH lines are morphologically and
  developmentally highly homogeneous (/?)

21
Field Performance of Double Haploids

• Conclusions
• - DH lines show limited variation in oil
  content/composition
• - limited evidence of inbreeding depression in
  dill
• - potential to commercialize selected DH lines

22
Whats next ?