Title: Umbilical Cord Stem Cell Therapy For Autism
1Umbilical Cord Stem Cell TherapyFor Autism
- David A. Steenblock, M.S., D.O.
- Steenblock Research Institute
- San Clemente, CA
2- Umbilical Cord
- Stem Cells
- A powerful, natural, safe,
- and ethical treatment!
- Considered Experimental for non-cancer diseases
-
3Some of The Successes
- J.H. is 56, and was diagnosed ten years ago with
progressive Multiple Sclerosis. Before
treatment, he was able to walk but needed a cane.
His speech was slurred and he had difficulty
swallowing.
4Multiple Sclerosis (continued)
- J.H. received purified umbilical cord stem cells
in July, 2003. In the first month following his
treatment, his muscle strength, his speech and
his swallowing dramatically improved.
5A.B. with Macular Degeneration
- A.B. was legally blind in his left eye due to
macular degeneration, central retinal vein
occlusions, optic atrophy from out of control
glaucoma and stroke complications. He also
struggled with crippling and terribly painful
arthritis - of both knees.
6Macular Degeneration (continued)
- In January 2006, RB received 10 million cord stem
cells and cord progenitor cells by direct
intra-arterial cerebral catheterization. - He also had two million mesenchymal stem cells
injected into his arthritic joints.
7Macular Degeneration (continued)
- Within three weeks, RB could see the food on his
plate so he could feed himself again. He could
again see his wife and see to get around.
Several months later, he was able to read street
signs again. - Most of the discomfort and pain from his
arthritic knees has disappeared.
8E.P. with Cerebral Palsy
- E.P. was born dead. She survived 28 minutes
without adequate oxygen which caused a stroke. - She had severe developmental delays and visual
problems. - E.P. received purified umbilical cord stem cells
in November, 2002.
9Cerebral Palsy (continued)
- E.P.s ability to focus, concentrate and
articulate have greatly improved. Four months
after the treatment, for the first time ever, she
could hold a crayon, draw a line, count to 24,
feed herself and interact with her sisters!
10- Umbilical cord stem cells have been found to be
beneficial in - Autism
- Cerebral Palsy
- Seizures
- Parkinsons disease
- Stroke
- Traumatic Brain Injury
- Diabetes, Type 1 and 2
- ALS, MS, ARMD, etc
- Research summaries available at
www.stemcelltherapies.org
11Case Studies (continued)
- Most of these cases are included in
- Umbilical Cord Stem Cell Therapy, the Gift of
Healing from - Healthy Newborns
- by Drs. Steenblock and Payne
- Now available through Basic Health Publications
(www.basichealthpub.com) - and www.Amazon.com
- Or call 949-248-7034
12Introduction to Stem Cells
13What are stem cells?
14Stem Cells
- At the beginning of life is the FIRST CELL.
-
- This cell, is totipotent
- i.e. capable of generating an entire new body
and any and all of the tissues within the body.
15Introduction to Stem Cells
- As the original totipotent stem cells begin to
- differentiate they next become
- multipotent cells
- These stem cells then further specialize into
- progenitor cells which finally become specific
- Differentiated, specialized, functioning tissues.
16Definition of a Stem Cell
- It can replicate itself (renewal)
- It can give rise to more specialized cells
(differentiation). - Stem cells home to areas of inflammation to
help repair and replace injured tissue. - Stem cells can give rise to a wide variety of
specialized cells, including heart cells, blood
cells, liver cells, and neurons. - Stem cells produce a number of growth substances
17Sources of Stem Cell Therapies
181. Embryonic Stem Cells
- Embryonic stem cells derived from fertilized eggs
- (has ethical, quality control and safety issues
can promote teratomas from rapid replication)
192. Adult Stem Cells
- Adult stem cells are obtained from bone marrow,
fat or blood. - e.g. two tablespoons of fat contain 10 million
mesenchymal stem cells.
203. Umbilical Cord Derived Stem Cells
- Umbilical cord stem cells are isolated by mixing
magnetic beads coated with specific antibodies
with fresh umbilical cord blood. The magnetic
bead-antibody attaches to the stem cells and are
removed by passing the mixture through a magnetic
collection column.
21Umbilical Cord Stem Cells (when processed by FDA
and AABB standards) appear to be the safest
type of stem cells for treating people.
22Umbilical Cord Stem Cells
- Umbilical cord blood stem cells have proven to be
safe in over 5000 cases treated worldwide since
1987 in treating children and adults with
cancer, leukemia and autoimmune disorders.
23Umbilical Cord Derived Stem Cells
- The blood from one umbilical cord contains about
300,000 multipotent stem cells.
24Umbilical Cord Stem Cells
- Umbilical cord stem cells have been considered
hemopoietic stem cells, related to their
ability to differentiate into blood cells.
25Umbilical Cord Stem Cells
- CD 34 cells are hemopoietic stem cells.
- A primitive subset - CD133 cells differentiate
into neurons. - Mesenchymal stem cells are also found in the
umbilical cord. - Primitive progenitors (comparable to embryonic
stem cells) are also found in umbilical cord
blood.
26Umbilical Cord Derived Stem Cells
- These various stem cells may divide into any type
of cell needed to heal an injury such as - blood cells
- Brain and spinal cord cells
- bone marrow cells
- immune cells
- liver cells
- pancreatic cells
- Eye tissues
- Blood vessels
- Etc.
27Cord Stem Cells Therapy Summary
- An ethical stem cell no loss of life required
- Good safety record
- Quality Control methodology is already in place
by the FDA and American Association of Blood
Banks (AABB). - Includes a variety of stem and progenitor cells
- Proving to be beneficial for brain injuries and
a number of other difficult to treat disorders
28The Steenblock Research Institute provides
technical support to international clinics who
- Get their stem cells from rigorously screened
cord blood. - The stem cells are separated from cord blood
- Culture in animal serum-free media (reduces graft
versus host immune reactions)
29Sources for the cord blood
- The cord blood is donated by healthy mothers in a
major hospital in the United States. - The cord blood is processed in a state and FDA
approved laboratory that uses state-of-the-art
technology to extract, expand, and freeze the
umbilical cord stem cells.
30Sources for the cord blood
- Each treatment vial contains about 2 million cord
stem cells. - There are indications from other cases treated in
Mexico that more stem cells produce better
results. - Therefore results for autism may be much better
with 10 million or more stem cells (5 or more
vials).
31How are the cord stem cells given?
- Most babies are given the stem cells via a
subcutaneous injection into the tissues adjacent
to the belly button. - Older children and adults are typically given
intravenous infusions. - Best results occur with high doses given by
intra-arterial catheter into the cerebral
arteries so all of the stem cells go directly to
the damaged brain tissue.
32When are effects noticed?
- Some patients have noticed changes within as
little as one hour following treatment. This may
be due to the release of growth factors. The
first changes are often seen in improved muscle
tone, lack of colds, increased energy, increased
attention span, and improved disposition. - Most people will not see changes for at least 3
weeks with the most profound results appearing
between the 3nd and 6th month.
33Mechanisms of Action
- CD 34 cord stem cells can release Glial derived
neurotrophic factor (GDNF). GDNF can rescue
neurons from a lack of oxygen and help stimulate
the repair of white matter in the brain. - CD34 stem cells can also release Neurotrophin 3
(NT-3), Nerve Growth Factor (NGF) and Brain
Derived Neurotrophic Factor (BDNF), which can
stimulate the growth of new neurons. - (Bracci-Laudiero L et al. J Neuroimmunol 2003
136(1-2) 130-9 - Chouthai NS et al. Prediatr Res 2003 53(6)
965-9)
34Cord Stem Cells also stimulate VEGF
- Vascular Endothelial Growth Factor (VEGF)
stimulates the production of stem cells
(endothelial progenitor cells) for new blood
vessels. - VEGF protects brain cells by inhibiting
programmed cell death (apoptosis). - VEGF stimulated hemopoietic stem cells have the
ability to promote the growth of new neurons to
repair and replace injured brain tissue. - Kim SY, et al. Differentiation of endothelial
cells form human umbilical cord blood AC133-CD14
cells. Ann Hematol 2005 84(7) 417-22. - Gora-Kupilas K, Josko J. The neuroprotective
function of vascular endothelial growth factor
(VEGF). FOLIA NEUROPATHOL 2005 43(1) 31-9.
35Mechanisms of Action (continued)
- Umbilical cord derived hemopoietic stem cells
(CD34/CD133) have a greater tendency to give
rise to glial cells and astrocytes, which help
repair white matter damage. - Cerebral palsy is characterized by white matter
damage and is the best responder to cord stem
cell treatments.
36Mechanisms of Action (continued)
- Stem Cells can also support existing injured
neurons by attaching to them, piggy back style. - This type of cell fusion may be more efficient
that creating new neurons in cases where neurons
may have hundreds of synapses already
established.
37Mechanisms of Action (continued)
- Cord stem cells (CD34, CD133, primitive
progenitors and mesenchymal stem cells) have all
been found to give rise to a small percentage of
neurons that can repair the gray matter of the
brain and spinal cord. - Piechaczek C. CD133. J Biol Regul Homeost
Agents 2001, 15(1) 101-2. - Hao HN, et al. Fetal human hematopoietic stem
cells can differentiate sequentially into neural
stem cells and then astrocytes in vitro. J
Hematother Stem Cell Res,2003, 12(1)23-32. - Ha Y, et al. Intermediate filament nestin
expressions in human cord blood monocytes
(HCMNCs). Acta Neurochir (Wien) 2003, 145(6)
483-7. - Handgretinger R, et al. Biology and plasticity of
CD133 hematopoietic stem cells. Ann NY Acad Sci
2003, 996 141-51. - Jang YK, et al. Retinoic acid-mediated induction
of neurons and glial cells from human umbilical
cord-derived hematopoietic stem cells. J
Neurosci Res 2004, 75(4) 573-84. - McGuckin CP, et al. Umbilical cord blood stem
cells can expand hematopoietic and neuroglial
progenitors in vitro. Exp Cell Res 2004, 295(2)
350-9. - Newman MB, et al. Human umbilical cord blood
(HUCB) cells for central nervous system repair.
Neurotox Res 2003, 5(5) 355-68. - Saporta S, et al. Human umbilical cord blood stem
cells infusion in spinal cord injury Engraftment
and beneficial influence on behavior. J
Hematother Stem Cell Res 2003, 12(3) 271-8. - Taguchi A, et al. Administration of CD34 cells
after stroke enhances neurogenesis and
angiogenesis in a mouse model. J Clin Invest
2004, 114(3) 330-8.
38Homing to the site of injury
- Stem cells home to or migrate towards specific
chemical signals given off by recently injured,
damaged or diseased tissues and organs (acute
cases). - There is always the danger that the stem cells
and progenitor cells can be destroyed by the free
radicals involved in the inflammation.
39Brain Injury and Autism
- A lack of blood flow to the left and right
temporal lobes is associated with primary autism.
This area is involved in the integration of
sensory and emotional information. It is also
the social brain, associated with social
perceptual skills often impaired in autism. - Bodaert N, et al. Superior temporal sulcus
anatomical abnormalities in childhood autism a
voxel-based morphometry MRI study. Neuroimage
2004 23(1) 364-9.
40Cord Stem Cells and Autism
- Umbilical cord stem cells can be of assistance in
cases that include a lack of blood flow to areas
of the brain (hypoperfusion).
41CD34 stem cells from umbilical cord blood have
the ability to
- Promote the growth of new blood vessels into the
damaged areas. - Promote the growth of new neurons.
- Help balance the immune system and autoimmune
disorders. - Relax the artery walls which promotes improved
blood flow.
42Pre-Treatments for Autism
- Remove heavy metals (mercury, lead, etc.)
- Eliminate infections
- Remove harmful germs from the intestines that
produce endotoxins which injure neurons and
blood vessels)
43Pre-Treatments for Autism
- Screen for nutritional deficiencies, autoimmune
problems, liver dysfunctions (haptoglobin,
alpha-2-macroglobulin, metalloproteins, Matrix
metalloproteinase-9, hormonal and growth factor
deficiencies, etc.) - Correct gastritis, duodenitis, colitis, stress
- Analyze for mutations and abnormal SNPs (single
nucleotide polymorphisms)
44Positive Responses
- The first autistic child we have followed was
6 years old and responded to an injection of only
1.5 million umbilical cord. - At first, he regressed, possibly from the stress
of the trip. There were behavioral problems,
bladder and bowel accidents and an increase in
fears. - Then he started acting more cheerful and peaceful
that ever before. He started singing, and
reduced his head banging and arm biting.
45Positive Responses (continued)
- He has become more interested in his
surroundings, exploring more, noticing the sounds
of airplanes. - He can follow multiple step directions now.
- Uses more complex sentences.
- His muscles are more relaxed.
- His ears are less sensitive to noise.
- There is more movement and adjustment possible
with his cranio sacral therapy.
46- Better results may occur with the use of
- Higher doses- 10 million or more stem/progenitor
cells may produce greater success. - Repetitive doses
- Adjunctive therapies such as
- Nerve growth factors
- Neuropeptides
- Magnetic Therapy
- Oxytocin, if needed
47- Better results
- A synergistic action occurs when more mesenchymal
stem cells are used in conjunction with the
neural progenitors.
48New Horizons in Autism Research
49When Nothing Else Works
50Gene Transfected Stem Cells
- Children usually die at about 6 years of age from
metachromatic leukodystrophy, a genetic disease
with a progressive deterioration of white matter
in the brain and spinal cord. - In the fall of 2005, Logan, aged 5, was
considered by her physicians to be terminal. - Her mother requested that Logan receive
genetically transfected stem cells in Mexico as
her only hope.
51Gene Transfected Stem Cells
- It took several months to test the transfected
stem cells in laboratory animals to make sure
they were safe. - Logan was treated with umbilical cord stem cells
that were used as a vector for the ARS-A gene
that the child was missing. - Several weeks after the treatment, Logan was
gaining muscle tone and was able to breathe by
herself for short periods without the respirator.
52Gene Transfected Stem Cells
- Since then our research team has worked with
other scientists to produce transfected stem
cells and test them for safety and effectiveness
in animal models for multiple sclerosis and ALS.
53Genetic Associations with Autism
- About 96 of Rett Syndrome cases are caused by
mutations or deletions in the MeCP2 gene.
Symptoms include mental retardation, autism and
perhaps X-linked disorders. - Zoghbi HY. MeCP2 dysfunction in humans and mice.
- J Child Neurol 2005 20(9) 736-40
54Genetic Associations with Autism
- With a 96 rate, there may be a good chance of
helping most Rett Syndrome children with MeCP2
transfected cord stem cells.
55Genetic Associations with Autism
- The reelin deficient mouse is used as a model for
autism. - An increase in free radical damage from toxic
insults reduce a liver enzyme called paraoxonase.
- This enzyme is required for liver detoxification
of lipid peroxides (oxidized fatty acids. - The gene for reelin glycoprotein is affected by
reductions in paraoxonase.
56Genetic Associations with Autism
- In the developing brain, reelin assists with
neural and glial migration. - In the adult brain, reelin is involved in signal
pathways involved with neurotransmission, memory
formation and synaptic function.
57Genetic Associations with Autism
- Reelin is also involved with GABA
neurotransmission which plays a role in
modulating the release of oxytocin, a hormone
associated with trust and empathy (too much,
however is related to repetitive behaviors).
58Genetic Associations with Autism
- Mutations and disruptions in the reelin gene may
underlie severe delay, seizures and cognitive and
social deficits associated with autism. - Bartlett CW, et al. Three autism candidate
genes a synthesis of human genetic analysis with
other disciplines. Int J Dev Neurosci 2005
23(2-3) 221-34 - Kirsch P, et al. Oxytocin modulates neural
circuitry for social cognition and fear in
humans. J Neurosci 2005 25(49) 11489-93. - Liu W, et al. Oxytocin receptors in brain
cortical regions are reduced in haploinsufficient
(/-) reeler mice. Neurol Res 2005 27(4)
339-45. - Serajee FJ, et al. Association of Feelin gene
polymorphisms with autism. Genomics 2006 87(1)
75-83. - Fatemi SH. Reelin glycoprotein structure,
biology and roles in health and disease. Mol
Psychiatry 2005 10(3) 251-7.
59Genetic Associations with Autism
- If other treatments are not effective, there is
the possibility of trying - paraoxonase transfected cord stem cells to help
with liver detoxification, if needed. - a reelin transfected cord stem cell to help with
neurotransmission, memory, severe delay, seizures
and social abilities.
60Genetic Associations with Autism
- The transfected stem cells are first tested in
laboratory animals for - safety and effectiveness.
61Genetic Associations with Autism
- The future of autism for difficult to treat cases
will be genetic testing for mutations and
polymorphisms - - then providing good genes
- through cord stem cells
62Genetic Associations with Autism
- Less prevalent gene mutations seen in autism
include the following
63Genetic Associations with Autism
- Chromosome 22q11.2 deletion is associated with
about 20 of autism spectrum symptoms. - Chromosome 2q21-33 associated with autism.
- Chromosome 16q is associated with autism.
- HOXA1 gene mutations
- Fine SE, et al. Autism spectrum disorders and
symptoms in children with molecularly confirmed
22q11.2 deletion syndrome. J Autism Dev Disord
2005 35(4) 461-70 - Posthuma D, et al. A genome wide scan for
intelligence identifies quantitative trait loci
on 2 q and 6p. Am J Hum Genet 2005 77(2)
318-26. - Philippi A, et al. Haplotypes in the gene
encoding protein kinase c-beta (PRKCB1) on
chromosome 16 are associated with autism. Mol
Psychiatry 2005 10(10) 950-601 - Tischfield MA, et al. Homozygous HOXA1 mutations
disrupt human brainstem, inner ear,
cardiovascular and cognitive development. Nat
Genet 2005 37(10) 1035-7
64Genetic Associations with Autism
- Mitochondrial dysfunction in several autistic
children is associated with chromosome 15q11-a13
inverted duplication. - Symptoms included moderate motor delay, lethargy,
severe hypotonia and modest lactic acidosis. - Filipek PA, et al. Mitochondrial dysfunction in
autistic patients with 15q inverted duplication.
Ann Neurol 2003 53(6) 801-4
65Umbilical Cord Stem Cell TherapiesThe Future
is Now
66For further information on research in umbilical
cord stem cell therapies
- Umbilical Cord Stem Cell Therapy,
- the Gift of Healing from Healthy Newborns by
Drs. Steenblock and Payne - www.basichealthpub.com
- www.amazon.com
67Steenblock Research Institute
- Donations for research tax deductible
- and gratefully accepted!
- Website http//www.stemcelltherapies.org
- Telephone 949 248-7034
- Thank you!