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Umbilical Cord Stem Cell Therapy For Autism


Umbilical cord stem cells are isolated by mixing magnetic beads coated with ... The blood from one umbilical cord contains about 300,000 'multipotent' stem cells. ... – PowerPoint PPT presentation

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Title: Umbilical Cord Stem Cell Therapy For Autism

Umbilical Cord Stem Cell TherapyFor Autism
  • David A. Steenblock, M.S., D.O.
  • Steenblock Research Institute
  • San Clemente, CA

  • Umbilical Cord
  • Stem Cells
  • A powerful, natural, safe,
  • and ethical treatment!
  • Considered Experimental for non-cancer diseases

Some of The Successes
  • J.H. is 56, and was diagnosed ten years ago with
    progressive Multiple Sclerosis. Before
    treatment, he was able to walk but needed a cane.
    His speech was slurred and he had difficulty

Multiple Sclerosis (continued)
  • J.H. received purified umbilical cord stem cells
    in July, 2003. In the first month following his
    treatment, his muscle strength, his speech and
    his swallowing dramatically improved.

A.B. with Macular Degeneration
  • A.B. was legally blind in his left eye due to
    macular degeneration, central retinal vein
    occlusions, optic atrophy from out of control
    glaucoma and stroke complications. He also
    struggled with crippling and terribly painful
  • of both knees.

Macular Degeneration (continued)
  • In January 2006, RB received 10 million cord stem
    cells and cord progenitor cells by direct
    intra-arterial cerebral catheterization.
  • He also had two million mesenchymal stem cells
    injected into his arthritic joints.

Macular Degeneration (continued)
  • Within three weeks, RB could see the food on his
    plate so he could feed himself again. He could
    again see his wife and see to get around.
    Several months later, he was able to read street
    signs again.
  • Most of the discomfort and pain from his
    arthritic knees has disappeared.

E.P. with Cerebral Palsy
  • E.P. was born dead. She survived 28 minutes
    without adequate oxygen which caused a stroke.
  • She had severe developmental delays and visual
  • E.P. received purified umbilical cord stem cells
    in November, 2002.

Cerebral Palsy (continued)
  • E.P.s ability to focus, concentrate and
    articulate have greatly improved. Four months
    after the treatment, for the first time ever, she
    could hold a crayon, draw a line, count to 24,
    feed herself and interact with her sisters!

  • Umbilical cord stem cells have been found to be
    beneficial in
  • Autism
  • Cerebral Palsy
  • Seizures
  • Parkinsons disease
  • Stroke
  • Traumatic Brain Injury
  • Diabetes, Type 1 and 2
  • ALS, MS, ARMD, etc
  • Research summaries available at

Case Studies (continued)
  • Most of these cases are included in
  • Umbilical Cord Stem Cell Therapy, the Gift of
    Healing from
  • Healthy Newborns
  • by Drs. Steenblock and Payne
  • Now available through Basic Health Publications
  • and
  • Or call 949-248-7034

Introduction to Stem Cells
What are stem cells?

Stem Cells
  • Fertilized Egg
  • At the beginning of life is the FIRST CELL.
  • This cell, is totipotent
  • i.e. capable of generating an entire new body
    and any and all of the tissues within the body.

Introduction to Stem Cells
  • As the original totipotent stem cells begin to
  • differentiate they next become
  • multipotent cells
  • These stem cells then further specialize into
  • progenitor cells which finally become specific
  • Differentiated, specialized, functioning tissues.

Definition of a Stem Cell
  • It can replicate itself (renewal)
  • It can give rise to more specialized cells
  • Stem cells home to areas of inflammation to
    help repair and replace injured tissue.
  • Stem cells can give rise to a wide variety of
    specialized cells, including heart cells, blood
    cells, liver cells, and neurons.
  • Stem cells produce a number of growth substances

Sources of Stem Cell Therapies
1. Embryonic Stem Cells
  • Embryonic stem cells derived from fertilized eggs
  • (has ethical, quality control and safety issues
    can promote teratomas from rapid replication)

2. Adult Stem Cells
  • Adult stem cells are obtained from bone marrow,
    fat or blood.
  • e.g. two tablespoons of fat contain 10 million
    mesenchymal stem cells.

3. Umbilical Cord Derived Stem Cells
  • Umbilical cord stem cells are isolated by mixing
    magnetic beads coated with specific antibodies
    with fresh umbilical cord blood. The magnetic
    bead-antibody attaches to the stem cells and are
    removed by passing the mixture through a magnetic
    collection column.

Umbilical Cord Stem Cells (when processed by FDA
and AABB standards) appear to be the safest
type of stem cells for treating people.
Umbilical Cord Stem Cells
  • Umbilical cord blood stem cells have proven to be
    safe in over 5000 cases treated worldwide since
    1987 in treating children and adults with
    cancer, leukemia and autoimmune disorders.

Umbilical Cord Derived Stem Cells
  • The blood from one umbilical cord contains about
    300,000 multipotent stem cells.

Umbilical Cord Stem Cells
  • Umbilical cord stem cells have been considered
    hemopoietic stem cells, related to their
    ability to differentiate into blood cells.

Umbilical Cord Stem Cells
  • CD 34 cells are hemopoietic stem cells.
  • A primitive subset - CD133 cells differentiate
    into neurons.
  • Mesenchymal stem cells are also found in the
    umbilical cord.
  • Primitive progenitors (comparable to embryonic
    stem cells) are also found in umbilical cord

Umbilical Cord Derived Stem Cells
  • These various stem cells may divide into any type
    of cell needed to heal an injury such as
  • blood cells
  • Brain and spinal cord cells
  • bone marrow cells
  • immune cells
  • liver cells
  • pancreatic cells
  • Eye tissues
  • Blood vessels
  • Etc.

Cord Stem Cells Therapy Summary
  • An ethical stem cell no loss of life required
  • Good safety record
  • Quality Control methodology is already in place
    by the FDA and American Association of Blood
    Banks (AABB).
  • Includes a variety of stem and progenitor cells
  • Proving to be beneficial for brain injuries and
    a number of other difficult to treat disorders

The Steenblock Research Institute provides
technical support to international clinics who
  • Get their stem cells from rigorously screened
    cord blood.
  • The stem cells are separated from cord blood
  • Culture in animal serum-free media (reduces graft
    versus host immune reactions)

Sources for the cord blood
  • The cord blood is donated by healthy mothers in a
    major hospital in the United States.
  • The cord blood is processed in a state and FDA
    approved laboratory that uses state-of-the-art
    technology to extract, expand, and freeze the
    umbilical cord stem cells.

Sources for the cord blood
  • Each treatment vial contains about 2 million cord
    stem cells.
  • There are indications from other cases treated in
    Mexico that more stem cells produce better
  • Therefore results for autism may be much better
    with 10 million or more stem cells (5 or more

How are the cord stem cells given?
  • Most babies are given the stem cells via a
    subcutaneous injection into the tissues adjacent
    to the belly button.
  • Older children and adults are typically given
    intravenous infusions.
  • Best results occur with high doses given by
    intra-arterial catheter into the cerebral
    arteries so all of the stem cells go directly to
    the damaged brain tissue.

When are effects noticed?
  • Some patients have noticed changes within as
    little as one hour following treatment. This may
    be due to the release of growth factors. The
    first changes are often seen in improved muscle
    tone, lack of colds, increased energy, increased
    attention span, and improved disposition.
  • Most people will not see changes for at least 3
    weeks with the most profound results appearing
    between the 3nd and 6th month.

Mechanisms of Action
  • CD 34 cord stem cells can release Glial derived
    neurotrophic factor (GDNF). GDNF can rescue
    neurons from a lack of oxygen and help stimulate
    the repair of white matter in the brain.
  • CD34 stem cells can also release Neurotrophin 3
    (NT-3), Nerve Growth Factor (NGF) and Brain
    Derived Neurotrophic Factor (BDNF), which can
    stimulate the growth of new neurons.
  • (Bracci-Laudiero L et al. J Neuroimmunol 2003
    136(1-2) 130-9
  • Chouthai NS et al. Prediatr Res 2003 53(6)

Cord Stem Cells also stimulate VEGF
  • Vascular Endothelial Growth Factor (VEGF)
    stimulates the production of stem cells
    (endothelial progenitor cells) for new blood
  • VEGF protects brain cells by inhibiting
    programmed cell death (apoptosis).
  • VEGF stimulated hemopoietic stem cells have the
    ability to promote the growth of new neurons to
    repair and replace injured brain tissue.
  • Kim SY, et al. Differentiation of endothelial
    cells form human umbilical cord blood AC133-CD14
    cells. Ann Hematol 2005 84(7) 417-22.
  • Gora-Kupilas K, Josko J. The neuroprotective
    function of vascular endothelial growth factor
    (VEGF). FOLIA NEUROPATHOL 2005 43(1) 31-9.

Mechanisms of Action (continued)
  • Umbilical cord derived hemopoietic stem cells
    (CD34/CD133) have a greater tendency to give
    rise to glial cells and astrocytes, which help
    repair white matter damage.
  • Cerebral palsy is characterized by white matter
    damage and is the best responder to cord stem
    cell treatments.

Mechanisms of Action (continued)
  • Stem Cells can also support existing injured
    neurons by attaching to them, piggy back style.
  • This type of cell fusion may be more efficient
    that creating new neurons in cases where neurons
    may have hundreds of synapses already

Mechanisms of Action (continued)
  • Cord stem cells (CD34, CD133, primitive
    progenitors and mesenchymal stem cells) have all
    been found to give rise to a small percentage of
    neurons that can repair the gray matter of the
    brain and spinal cord.
  • Piechaczek C. CD133. J Biol Regul Homeost
    Agents 2001, 15(1) 101-2.
  • Hao HN, et al. Fetal human hematopoietic stem
    cells can differentiate sequentially into neural
    stem cells and then astrocytes in vitro. J
    Hematother Stem Cell Res,2003, 12(1)23-32.
  • Ha Y, et al. Intermediate filament nestin
    expressions in human cord blood monocytes
    (HCMNCs). Acta Neurochir (Wien) 2003, 145(6)
  • Handgretinger R, et al. Biology and plasticity of
    CD133 hematopoietic stem cells. Ann NY Acad Sci
    2003, 996 141-51.
  • Jang YK, et al. Retinoic acid-mediated induction
    of neurons and glial cells from human umbilical
    cord-derived hematopoietic stem cells. J
    Neurosci Res 2004, 75(4) 573-84.
  • McGuckin CP, et al. Umbilical cord blood stem
    cells can expand hematopoietic and neuroglial
    progenitors in vitro. Exp Cell Res 2004, 295(2)
  • Newman MB, et al. Human umbilical cord blood
    (HUCB) cells for central nervous system repair.
    Neurotox Res 2003, 5(5) 355-68.
  • Saporta S, et al. Human umbilical cord blood stem
    cells infusion in spinal cord injury Engraftment
    and beneficial influence on behavior. J
    Hematother Stem Cell Res 2003, 12(3) 271-8.
  • Taguchi A, et al. Administration of CD34 cells
    after stroke enhances neurogenesis and
    angiogenesis in a mouse model. J Clin Invest
    2004, 114(3) 330-8.

Homing to the site of injury
  • Stem cells home to or migrate towards specific
    chemical signals given off by recently injured,
    damaged or diseased tissues and organs (acute
  • There is always the danger that the stem cells
    and progenitor cells can be destroyed by the free
    radicals involved in the inflammation.

Brain Injury and Autism
  • A lack of blood flow to the left and right
    temporal lobes is associated with primary autism.
    This area is involved in the integration of
    sensory and emotional information. It is also
    the social brain, associated with social
    perceptual skills often impaired in autism.
  • Bodaert N, et al. Superior temporal sulcus
    anatomical abnormalities in childhood autism a
    voxel-based morphometry MRI study. Neuroimage
    2004 23(1) 364-9.

Cord Stem Cells and Autism
  • Umbilical cord stem cells can be of assistance in
    cases that include a lack of blood flow to areas
    of the brain (hypoperfusion).

CD34 stem cells from umbilical cord blood have
the ability to
  • Promote the growth of new blood vessels into the
    damaged areas.
  • Promote the growth of new neurons.
  • Help balance the immune system and autoimmune
  • Relax the artery walls which promotes improved
    blood flow.

Pre-Treatments for Autism
  • Remove heavy metals (mercury, lead, etc.)
  • Eliminate infections
  • Remove harmful germs from the intestines that
    produce endotoxins which injure neurons and
    blood vessels)

Pre-Treatments for Autism
  • Screen for nutritional deficiencies, autoimmune
    problems, liver dysfunctions (haptoglobin,
    alpha-2-macroglobulin, metalloproteins, Matrix
    metalloproteinase-9, hormonal and growth factor
    deficiencies, etc.)
  • Correct gastritis, duodenitis, colitis, stress
  • Analyze for mutations and abnormal SNPs (single
    nucleotide polymorphisms)

Positive Responses
  • The first autistic child we have followed was
    6 years old and responded to an injection of only
    1.5 million umbilical cord.
  • At first, he regressed, possibly from the stress
    of the trip. There were behavioral problems,
    bladder and bowel accidents and an increase in
  • Then he started acting more cheerful and peaceful
    that ever before. He started singing, and
    reduced his head banging and arm biting.

Positive Responses (continued)
  • He has become more interested in his
    surroundings, exploring more, noticing the sounds
    of airplanes.
  • He can follow multiple step directions now.
  • Uses more complex sentences.
  • His muscles are more relaxed.
  • His ears are less sensitive to noise.
  • There is more movement and adjustment possible
    with his cranio sacral therapy.

  • Better results may occur with the use of
  • Higher doses- 10 million or more stem/progenitor
    cells may produce greater success.
  • Repetitive doses
  • Adjunctive therapies such as
  • Nerve growth factors
  • Neuropeptides
  • Magnetic Therapy
  • Oxytocin, if needed

  • Better results
  • A synergistic action occurs when more mesenchymal
    stem cells are used in conjunction with the
    neural progenitors.

New Horizons in Autism Research
When Nothing Else Works
Gene Transfected Stem Cells
  • Children usually die at about 6 years of age from
    metachromatic leukodystrophy, a genetic disease
    with a progressive deterioration of white matter
    in the brain and spinal cord.
  • In the fall of 2005, Logan, aged 5, was
    considered by her physicians to be terminal.
  • Her mother requested that Logan receive
    genetically transfected stem cells in Mexico as
    her only hope.

Gene Transfected Stem Cells
  • It took several months to test the transfected
    stem cells in laboratory animals to make sure
    they were safe.
  • Logan was treated with umbilical cord stem cells
    that were used as a vector for the ARS-A gene
    that the child was missing.
  • Several weeks after the treatment, Logan was
    gaining muscle tone and was able to breathe by
    herself for short periods without the respirator.

Gene Transfected Stem Cells
  • Since then our research team has worked with
    other scientists to produce transfected stem
    cells and test them for safety and effectiveness
    in animal models for multiple sclerosis and ALS.

Genetic Associations with Autism
  • About 96 of Rett Syndrome cases are caused by
    mutations or deletions in the MeCP2 gene.
    Symptoms include mental retardation, autism and
    perhaps X-linked disorders.
  • Zoghbi HY. MeCP2 dysfunction in humans and mice.
  • J Child Neurol 2005 20(9) 736-40

Genetic Associations with Autism
  • With a 96 rate, there may be a good chance of
    helping most Rett Syndrome children with MeCP2
    transfected cord stem cells.

Genetic Associations with Autism
  • The reelin deficient mouse is used as a model for
  • An increase in free radical damage from toxic
    insults reduce a liver enzyme called paraoxonase.
  • This enzyme is required for liver detoxification
    of lipid peroxides (oxidized fatty acids.
  • The gene for reelin glycoprotein is affected by
    reductions in paraoxonase.

Genetic Associations with Autism
  • In the developing brain, reelin assists with
    neural and glial migration.
  • In the adult brain, reelin is involved in signal
    pathways involved with neurotransmission, memory
    formation and synaptic function.

Genetic Associations with Autism
  • Reelin is also involved with GABA
    neurotransmission which plays a role in
    modulating the release of oxytocin, a hormone
    associated with trust and empathy (too much,
    however is related to repetitive behaviors).

Genetic Associations with Autism
  • Mutations and disruptions in the reelin gene may
    underlie severe delay, seizures and cognitive and
    social deficits associated with autism.
  • Bartlett CW, et al. Three autism candidate
    genes a synthesis of human genetic analysis with
    other disciplines. Int J Dev Neurosci 2005
    23(2-3) 221-34
  • Kirsch P, et al. Oxytocin modulates neural
    circuitry for social cognition and fear in
    humans. J Neurosci 2005 25(49) 11489-93.
  • Liu W, et al. Oxytocin receptors in brain
    cortical regions are reduced in haploinsufficient
    (/-) reeler mice. Neurol Res 2005 27(4)
  • Serajee FJ, et al. Association of Feelin gene
    polymorphisms with autism. Genomics 2006 87(1)
  • Fatemi SH. Reelin glycoprotein structure,
    biology and roles in health and disease. Mol
    Psychiatry 2005 10(3) 251-7.

Genetic Associations with Autism
  • If other treatments are not effective, there is
    the possibility of trying
  • paraoxonase transfected cord stem cells to help
    with liver detoxification, if needed.
  • a reelin transfected cord stem cell to help with
    neurotransmission, memory, severe delay, seizures
    and social abilities.

Genetic Associations with Autism
  • The transfected stem cells are first tested in
    laboratory animals for
  • safety and effectiveness.

Genetic Associations with Autism
  • The future of autism for difficult to treat cases
    will be genetic testing for mutations and
    polymorphisms -
  • then providing good genes
  • through cord stem cells

Genetic Associations with Autism
  • Less prevalent gene mutations seen in autism
    include the following

Genetic Associations with Autism
  • Chromosome 22q11.2 deletion is associated with
    about 20 of autism spectrum symptoms.
  • Chromosome 2q21-33 associated with autism.
  • Chromosome 16q is associated with autism.
  • HOXA1 gene mutations
  • Fine SE, et al. Autism spectrum disorders and
    symptoms in children with molecularly confirmed
    22q11.2 deletion syndrome. J Autism Dev Disord
    2005 35(4) 461-70
  • Posthuma D, et al. A genome wide scan for
    intelligence identifies quantitative trait loci
    on 2 q and 6p. Am J Hum Genet 2005 77(2)
  • Philippi A, et al. Haplotypes in the gene
    encoding protein kinase c-beta (PRKCB1) on
    chromosome 16 are associated with autism. Mol
    Psychiatry 2005 10(10) 950-601
  • Tischfield MA, et al. Homozygous HOXA1 mutations
    disrupt human brainstem, inner ear,
    cardiovascular and cognitive development. Nat
    Genet 2005 37(10) 1035-7

Genetic Associations with Autism
  • Mitochondrial dysfunction in several autistic
    children is associated with chromosome 15q11-a13
    inverted duplication.
  • Symptoms included moderate motor delay, lethargy,
    severe hypotonia and modest lactic acidosis.
  • Filipek PA, et al. Mitochondrial dysfunction in
    autistic patients with 15q inverted duplication.
    Ann Neurol 2003 53(6) 801-4

Umbilical Cord Stem Cell TherapiesThe Future
is Now
For further information on research in umbilical
cord stem cell therapies
  • Umbilical Cord Stem Cell Therapy,
  • the Gift of Healing from Healthy Newborns by
    Drs. Steenblock and Payne

Steenblock Research Institute
  • Donations for research tax deductible
  • and gratefully accepted!
  • Website http//
  • Telephone 949 248-7034
  • Thank you!