1 control of glycogen metabolism

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Protein Structure, Function Regulation
Programme
Research Activities

• (1) control of glycogen metabolism
• (2) control of neuromuscular transmission
• Glycogen phosphorylase inhibitors as potential
  antidiabetic drugs
• Structure and function of phosphorylase kinase
• Crystallographic studies on Fab fragments of mAbs
  against the MIR of AChR

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Protein Structure, Function Regulation
Programme
Main Achievements

• Structure and function of phosphorylase kinase
• Role of Asp149 in binding and orientation of
  the substrate and in catalysis of phosphoryl
  transfer. Activation segment is necessary in
  order to recognise and present the protein
  substrate for catalysis.
• Crystallographic studies on Fab fragments of
  mAbs against the MIR of AChR
• Crystal structures of the Fab fragments of rat
  monoclonal antibodies, 192 and 198. Rational
  design of very high affinity mutants
• Glycogen phosphorylase (GP) inhibitors as
  potential antidiabetic drugs

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Protein Structure, Function Regulation
Programme
Achievements

• 19 Publications (refereed)
• 425 Citations
• 4 Ph.D Theses
• 11 Externally Funded Projects, 108.000.000 GRD
• Collaboration with
• Pfizer (USA)
• Novo Nordisk (Denmark)
• Aventis (Germany).

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Protein Structure, Function Regulation
Programme
Perspectives(1)

• Development of potential antidiabetic drugs
• Specific inhibitors for calcium/calmodulin
  regulated kinases by knowledge based design and
  combinatorial chemistry
• Rational Design of Ribonuclease Inhibitors
• Structural Basis of Nicotinic Acetylcholine
  Receptor Function

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Protein Structure, Function Regulation
Programme
Perspectives(2)

• European Structural Biology Forum (ESBF)- to a
  European infrastructure co-operation network
  (European Research Infrastructures Users)
• Equipment for Structural Biology
• Crystallisation room
• 3 Silicon Graphics O2 Workstations
• High-end V-Class V2600 supercomputer
• Image Plate R-AXIS IV, CDSB, SRS, DESY, ELETTRA

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Protein Structure, Function Regulation
Programme
Staff
Nikos G. Oikonomakos, Head Spyros E.
Zographos, Post-doc Katerina E. Tsitsanou, Res.
Assistant Vicky T. Skamnaki, Res. Assistant Eleni
Averkiou, technician
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Protein Structure, Function Regulation
Programme
Collaborations

• Prof. Louise N. Johnson, Lab. of Molecular
  Biophysics, Univ. of Oxford
• Prof. George W.J. Fleet, Dyson Perrins
  Laboratory, Univ. of Oxford
• Prof. Socrates J. Tzartos, Biochemistry Dept.,
  Hellenic Pasteur Instit., Athens
• Prof. Costantinos Sakarellos, Dept. of
  Chemistry, Univ. of Ioannina
• Dr. Patricia T.W. Cohen, Dept. of Biochemistry,
  Univ. of Dundee
• Prof. David Barford, Struct. Biology Section,
  Inst. of Cancer Research, London
• Prof. G. Eisenbrand, Fachbereich Chemie, Univ.
  Kaiserslautem
• Prof. G. Archontis, Dept. of Physics, Univ. of
  Cyprus
• Prof. Martin Karplus, Dept. of Chemistry Chem.
  Biol., Harvard Univ
• Dr. Virginia L. Rath, Dept. of Explor. Med.
  Sciences, Pfizer Inc., USA