TATA box. DNA (a) Little or no transcription. Enhancer. DNA. TATA box. Activator (transcription factor) (b) High rate of transcription. Figure 13-10 ...
'Modeling network dynamics: the lac operon, a case study' ... Bacteriophage l. corona virus. HIV. smallpox. influenza. Attractive, but nasty.... Lysis ...
Negative control: repressor protein present which prevents ... The repressor is an allosteric protein that can be activated by binding to a small molecule ...
The structural genes (any gene other than a regulator) for enzymes involved in a ... sfactors to RNA polymerase can confer a new promoter specificity on the enzyme ...
The Lac Operon 1961, Jacob and Monod E. coli and other bacteria Bacterial Genes Many genes constitutively expressed housekeeping genes Other genes are more ...
REGULATION OF TRANSCRIPTION IN PROKARYOTES. CLEAVAGE OF LACTOSE TO. GALACTOSE AND GLUCOSE ... IPTG IS A GRATUITOUS' lac. OPERON INDUCER. WEAVER: FIG. 7.2 & 7.4 ...
We will be using the lac operon (a series of three genes linked together) to ... The lac operon is a prokaryotic ... THE LAC OPERON AND ITS BIOCHEMICAL ROLE ...
In absence of lactose the lac operon is. repressed - only a few copies of b-gal ... Mutations in I gene (upstream of lac. operon) or operator cause constitutive ...
What is lactose & where do we find it? Carbohydrate, disaccharide, glucose galactose; dairy ... Grasshopper. XY. XX. Birds. XX. XY. Human. Female. Male ...
... is the inducer molecule responsible for the increased production of ... The positive control system turn on the expression of the operon ensuring that ...
You will soon be eating tons of turkey, a rich source of tryptophan. ... Allolactose, an isomer of lactose, which is present in small amounts, controls that lac operon ...
This learning object has been funded by the European Commissions FP6 BioMinE project ... gene lacZ : b-galactosidase splits lactose into glycose galactose ...
4. CAP must release from lac promoter DNA ... Terminator. 1-2. 3-4. 2-3. Anti-Terminator. Prevents formation. of 3-4 stem loop. trp operon: summary ...
... of the CRP-cAMP complex bound ... also subject to POSITIVE regulation, by CRP in the presence of cAMP ... to the activity of CRP which activates lac operon in ...
Regulation of Gene Expression in Prokaryotes * * How Prokaryotes Regulate How Their Genes are Expressed Possible points of regulation: 1. Block transcription of the ...
Expression blocked by a repressor that can be inactivated by a small molecule called an inducer ... to be expressed in inducible fashion with lactose as inducer ...
Encodes repressible anabolic enzymes involved in the production of the amino acid arginine ... in the arginine biosynthetic pathway. In the absence of arginine ...
Title: Lecture #8 Date _____ Author: Chris Hilvert Last modified by: CCSD Created Date: 11/1/2000 3:32:59 PM Document presentation format: On-screen Show (4:3)
The Central Dogma of Molecular Biology REPLICATION Development of multicellular organisms Totipotency Patterns of gene expression Gene structure What is required for ...
Lactose induces enzyme expression by binding to the LacI repressor preventing ... Glucose is more rapidly metabolized than lactose, and is a preferred carbon source ...
Control of Gene Expression in Bacteria Gene Regulation and Information Flow: an overview Metabolizing Lactose A Model System: multiple genes, classes of mutants ...
Translation mRNA exits the nucleus through the nuclear pores In the cytoplasm, it joins with the other key players to assemble a polypeptide. The other parts of the ...
Gene Regulation Gene Regulation in Prokaryotes the Jacob-Monad Model certain genes are transcribed all the time constitutive genes synthesis of some proteins ...
Ch. 10 Notes DNA: Transcription and Translation GOALS Compare the structure of RNA with that of DNA Summarize the process of transcription Relate the role of codons ...
It binds to DNA sequence of operator, prevents binding of RNA polymerase to promoter. ... Methylation of DNA bases promotes condensation while acetylation of histone ...
Molecular genetics of bacteria Gene regulation and regulation of metabolism ... transfer of DNA via a virus. More common, but still requires close relative.
Gene Regulation Section 12 5 This section explains how some genes in prokaryotes and eukaryotes are controlled. Gene Structure Gene Regulation: Where does RNA ...
2. Press velvet-covered block against master plate. Some cells transfer from plate to block. ... Velvet-covered block. Lactose only. Replica plate. Lactose only ...
Transcription Central Dogma Genes Sequence of DNA that is transcribed. Encode proteins, tRNAs, rRNAs, etc.. Housekeeping genes encode proteins or RNAs that are ...
bistable. monostable. monostable. initial LOW state. initial HIGH state. Mapping the bistable region as a function of TMG and glucose concentration. Ozbudak et al. ...
seoul subway. tokyo subway. pyrimidine pathway. protein pathway. from DNA to pathways ... programmed cell applications. biological cell programming. biological ...
Introduction to DNA Sequencing. 2. Determine which base ... containing mutated lacZ. F' Episome. Trans-Complementation. E. coli. D(lacZ)M15. Non-Functional ...
Used extensively for mapping contact points between promoter sequences and RNA ... keratin in skin and hair follicle cells. muscle actin and myosin in muscle cells ...
Regulation makes sense- the bacteria/cell does the logical, intelligent thing ... The enzyme that produces cAMP (adenyl cyclase), is inhibited by allosteric ...
Enhancers and enhancer-binding proteins activate transcription reminiscent of ... to 2 enhancers and RNA polymerase II bound to the promoter and the ...
Activator binds to DNA sites called enhancer to enhance. the RNA polymerase activity. ... enhancer in higher eukaryotes, upstream activator sequences (UASs) in yeast. ...