TATA box. DNA (a) Little or no transcription. Enhancer. DNA. TATA box. Activator (transcription factor) (b) High rate of transcription. Figure 13-10 ...
'Modeling network dynamics: the lac operon, a case study' ... Bacteriophage l. corona virus. HIV. smallpox. influenza. Attractive, but nasty.... Lysis ...
Negative control: repressor protein present which prevents ... The repressor is an allosteric protein that can be activated by binding to a small molecule ...
The Lac Operon 1961, Jacob and Monod E. coli and other bacteria Bacterial Genes Many genes constitutively expressed housekeeping genes Other genes are more ...
The structural genes (any gene other than a regulator) for enzymes involved in a ... sfactors to RNA polymerase can confer a new promoter specificity on the enzyme ...
... the promoter for the operon containing the arabinose or galactose ... O2 and I1 half sites of the araBAD operon, forming a. 210 bp DNA loop (Figure 1) ...
REGULATION OF TRANSCRIPTION IN PROKARYOTES. CLEAVAGE OF LACTOSE TO. GALACTOSE AND GLUCOSE ... IPTG IS A GRATUITOUS' lac. OPERON INDUCER. WEAVER: FIG. 7.2 & 7.4 ...
Chapter 15 Gene Expression ... then take the on-line quiz together as a class Overview of Inducible Lac Operon Inducible Lac operon TWO regulatory mechanisms ...
We will be using the lac operon (a series of three genes linked together) to ... The lac operon is a prokaryotic ... THE LAC OPERON AND ITS BIOCHEMICAL ROLE ...
In absence of lactose the lac operon is. repressed - only a few copies of b-gal ... Mutations in I gene (upstream of lac. operon) or operator cause constitutive ...
What is lactose & where do we find it? Carbohydrate, disaccharide, glucose galactose; dairy ... Grasshopper. XY. XX. Birds. XX. XY. Human. Female. Male ...
... is the inducer molecule responsible for the increased production of ... The positive control system turn on the expression of the operon ensuring that ...
You will soon be eating tons of turkey, a rich source of tryptophan. ... Allolactose, an isomer of lactose, which is present in small amounts, controls that lac operon ...
This learning object has been funded by the European Commissions FP6 BioMinE project ... gene lacZ : b-galactosidase splits lactose into glycose galactose ...
4. CAP must release from lac promoter DNA ... Terminator. 1-2. 3-4. 2-3. Anti-Terminator. Prevents formation. of 3-4 stem loop. trp operon: summary ...
... of the CRP-cAMP complex bound ... also subject to POSITIVE regulation, by CRP in the presence of cAMP ... to the activity of CRP which activates lac operon in ...
Regulation of Gene Expression in Prokaryotes * * How Prokaryotes Regulate How Their Genes are Expressed Possible points of regulation: 1. Block transcription of the ...
Title: Lecture #8 Date _____ Author: Chris Hilvert Last modified by: CCSD Created Date: 11/1/2000 3:32:59 PM Document presentation format: On-screen Show (4:3)
Expression blocked by a repressor that can be inactivated by a small molecule called an inducer ... to be expressed in inducible fashion with lactose as inducer ...
Encodes repressible anabolic enzymes involved in the production of the amino acid arginine ... in the arginine biosynthetic pathway. In the absence of arginine ...
The Central Dogma of Molecular Biology REPLICATION Development of multicellular organisms Totipotency Patterns of gene expression Gene structure What is required for ...
Lactose induces enzyme expression by binding to the LacI repressor preventing ... Glucose is more rapidly metabolized than lactose, and is a preferred carbon source ...
Control of Gene Expression in Bacteria Gene Regulation and Information Flow: an overview Metabolizing Lactose A Model System: multiple genes, classes of mutants ...
Translation mRNA exits the nucleus through the nuclear pores In the cytoplasm, it joins with the other key players to assemble a polypeptide. The other parts of the ...
Chapter 16 Control of Gene Expression in Prokaryotes Genes Structural genes Code for proteins involved with general processes (metabolism, catabolism) or ...
Gene Regulation Gene Regulation in Prokaryotes the Jacob-Monad Model certain genes are transcribed all the time constitutive genes synthesis of some proteins ...
Molecular genetics of bacteria Gene regulation and regulation of metabolism Genetic exchange among bacteria Bacteria are successful because They carefully regulate ...
Ch. 10 Notes DNA: Transcription and Translation GOALS Compare the structure of RNA with that of DNA Summarize the process of transcription Relate the role of codons ...
Gene Regulation Section 12 5 This section explains how some genes in prokaryotes and eukaryotes are controlled. Gene Structure Gene Regulation: Where does RNA ...
... after polymerization of about 200 nucleotides) if the entire messenger if not ... because it saves the polymerization of several thousand nucleotides in ...
It binds to DNA sequence of operator, prevents binding of RNA polymerase to promoter. ... Methylation of DNA bases promotes condensation while acetylation of histone ...
Molecular genetics of bacteria Gene regulation and regulation of metabolism ... transfer of DNA via a virus. More common, but still requires close relative.
Gene Regulation Section 12 5 This section explains how some genes in prokaryotes and eukaryotes are controlled. Gene Structure Gene Regulation: Where does RNA ...
Microbial Genetics Chapter 8 Structure ... no disease Transduction DNA is transferred from one bacterium to another by a virus Bacteriophage virus that only infects bacteria ...
2. Press velvet-covered block against master plate. Some cells transfer from plate to block. ... Velvet-covered block. Lactose only. Replica plate. Lactose only ...
Transcription Central Dogma Genes Sequence of DNA that is transcribed. Encode proteins, tRNAs, rRNAs, etc.. Housekeeping genes encode proteins or RNAs that are ...