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Recombination Definitions Models Mechanisms Definition of recombination Breaking and rejoining of two parental DNA molecules to produce new DNA molecules Types of ...
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Homologous Recombination: Presynaptic Filaments & Brca2 Scott Morrical Dept. of Biochemistry smorrica@zoo.uvm.edu E. coli RecA Paradigm Eukaryotic Rad51
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Chapter 5 Bacterial genetics
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Bacterial Genomes Remember no nucleus!! Bacterial chromosome - Large ds circular DNA molecule = haploid - E. coli has about 4,300 genes (~4.2 Mb)
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Microbiological control of medicines in pharmaceutical manufacturing and pharmaceutical companies. Fundamentals of biotechnology and genetic engineering.
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Title: Bacterial Genetics Author: g-max Last modified by: uoj Created Date: 10/16/2004 12:40:04 PM Document presentation format: On-screen Show Other titles
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100x more DNA than the average virus. 1000x less DNA than ... Avirulent. a-hemolysis. of RBCs. Blood Agar Plate. No capsule! 12. 13. The Transforming Principle ...
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Breaking and rejoining of two parental DNA molecules to produce new DNA molecules ... to anaphase I can lead to aberrant numbers of chromosomes, e.g. trisomy for ...
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Bacterial Genetics Review Genome ... Transduction Bacterial DNA is transferred via a virus -Bacteriophage Virulent phages lytic cycle Generalized transduction Any gene ...
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Homologous recombination of E. coli Identification of genes involved in ... isolation of mutants affecting recombination in recB recC sbcB or recB ...
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A section of DNA that has enough information to make one protein is ... BST. Genetically modified foods. Bioremediation. How? Use enzymes to cut plasmid DNA ...
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Bacterial Plasmids and Conjugation. Chapter 13: pp. 288 291; 296 299. Characteristics ... be transferred. Recombination or Genetic variability. Conjugation ...
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Bacterial Genetics Transformation ... be triggered DNA segments must be small ~ 500 kb Transformation Transformation Transformation Transduction Temperate virus attacks ...
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Bacterial Genetics Xiao-Kui GUO PhD ... Transduction is defined as the transfer of genetic information between cells through the mediation of a virus ...
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Variation in bacteria mainly caused by mutation ... Anatomy of the lac operon ... Effect of lactose on the lac operon. Energy preference of E.coli ...
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Recombination, Bacteriophages, and Horizontal Gene Transfer 2005 Bacterial Conjugation transfer of DNA by direct cell to cell contact discovered 1946 by Lederberg and ...
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Title: PowerPoint Created Date: 10/27/2003 7:50:53 AM Document presentation format: Other titles: Times New Roman Tahoma ...
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Integration of DNA (from another organism or genetic element) into ... Replication and transfer of F plasmid by rolling circle replication (semiconservative) ...
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1. Addressable Bacterial Conjugation. UC Berkeley iGEM 2005. Michael Chen. Vlad Goldenberg. Stephen Handley. Melissa Li. Jonathan Sternberg. Jay Su. Eddie Wang ...
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... minimal media 190 carbon sources ... Number of genes type Strains 1 5,289,471 5,349 EHEC EDL933 109 5,516,160 4,788 EPEC E22 1 5,498,450 5,361 EHEC ...
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Structure, Replication and Recombination of DNA
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lysis: splitting open of the bacteria (ie. to release phage) ... 1 bacteria lyses, releasing 100's of phage, they infect nearby bacteria, etc ...
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Instead, bacteria acquire DNA from other bacteria through three distinct mechanisms: ... Ames Salmonella Test. DNA-Mediated Transformation ...
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Digest with Restriction Enzymes. Get RFLPs. Place into electrophoresis chamber. Separation based on size and charge. Stain gel ...
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Greater variety in offspring: Generates new combinations of alleles. Negative selection can remove deleterious alleles from a population without ...
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Many studies have been done to provide different delivery systems ... E-coli. Mannheimia haemolytica. Actinobacillus pleuropneumoniae. Pasteurella haemolytica ...
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Bacterial Genetics (Ch18) Genetic diversity caused by Rapid reproduction Mutation Recombination Bacteria One of the simplest genetic model systems to study the ...
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To establish specific cell-to-cell communication between two populations of bacteria ... Dr. Laura Frost: F-Plasmid. Philip Silverman: pox38 F-Plasmid ...
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and thus lead to a more Dyn-amic genome structure. Introduce ... its dual action as a stimulatory cofactor for excision and an inhibitor of ...
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Bacterial Classification, Anatomy, Nutrition, Growth, Metabolism and Genetics Electronic Counting Microbial genetics Genomes Prokaryotic Genomes Prokaryotic ...
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Bacterial Genetics Bacteria are essential organisms in demonstrating structure and function of DNA: Their haploid genetic state allows expression of a genetic trait ...
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Cipro 500mg (Generic Ciprofloxacin) is the best medicine used to prevent bacterial infections. The extended release tablet of cipro is used to treat various types of urinary tract infections. Cipro (Ciprofloxacin) is a medication used in the treatment of anthrax which is measured to be a serious infection.
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Genetic Recombination Definition: The breakage and joining of DNA into new combinations Purposes Promotes genetic diversity within a species - within a chromosome ...
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Can be grown both in liquid and agar medium, colony = visible cluster of cells ... On rare occasions, the F plasmid is integrated into the circular chromosome, and ...
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Bacterial expression System in Creative BioMart
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Bacterial expression System in Creative BioMart
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Bacterial expression System in Creative BioMart
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Text: pages 88-90, 95-103, 108-109 Growth and nutrition. Text: pages 541-544 E. coli ... 4 Septum cell wall synthesis 'Fts' proteins. 5 Cell wall separation ...
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process in which one or more nucleic acid molecules are rearranged or combined ... represented as BOB', where B and B' represent the bacterial DNA on either side ...
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Recombinant DNA Bacterial Transformation Student Instructions Ligation Recombinant Transformation Ligation Obtain reagent tubes labeled: - pAmp (digested) - pKan ...
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Eukaryotes are defined as having their genetic material enclosed in a membrane ... bound organelles such as mitochondria, lysosomes, and endoplasmic reticulum. ...
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Contain nuclease (exonuclease and Chi-specific ... recR, recQ, recJ ... RecA forms filaments with single-stranded DNA and catalyzes the assimilation of ...
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bcterial conjugation mating hsr f plus f minuas f prime
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Bacterial genetics, lectures 3 ST Replication -DNA Regulation Change - mutation - gene exchange
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Bacterial Transformation of pGLO Lab Background Gene Regulation Review Organisms regulate expression of their genes and ultimately the amounts and kinds of proteins ...
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Genetic Recombination and Genetic Engineering
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Analyze how a gene can transform an organism and express that gene ... Bacteria present with whitish colonies (regeneration of the starter plate) -pGlo. LB only ...
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Title: PowerPoint Presentation - Recombinant DNA Bacterial Transformation-Student Instructions Plasmid Digest Author: Mike Zeller Last modified by
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Bacterial Transformation of pGLO Lab Background Gene Regulation Review Organisms regulate expression of their genes and ultimately the amounts and kinds of proteins ...
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Title: PowerPoint Presentation Author: Anil Last modified by: Todd Eckdahl Created Date: 2/8/2001 8:28:33 PM Document presentation format: Custom Company
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3.DNA cleavage via a transesterification reaction can also occur between two ... use this same site to catalyze both the DNA cleavage and the DNA strand transfer ...
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Chapter 16 Recombination DNA and Genetic Engineering Animal Organs as Biotechnology Products 1. It may be possible to use genetically engineered pigs to serve as a ...
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Recombination, Phase Variation and Antigenic Variation Why Recombination? Homologous Recombination General Recombination often involves a Holliday Junction Proposed ...
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Title: PowerPoint Created Date: 10/27/2003 7:50:53 AM Document presentation format: Other titles: Times New Roman Tahoma ...
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Title: PowerPoint Presentation Last modified by: Davidson College Document presentation format: On-screen Show Company: Davidson College Other titles
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e.g. Purified DNA sequences could be specifically labeled by nick translation ... To paraphrase Gertrude Stein.... DNA is DNA is DNA... Identify this molecule ...
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