E,coli and Agri Ionization Systems to combat bacteria, pathogens and virus. - PowerPoint PPT Presentation

Title: E,coli and Agri Ionization Systems to combat bacteria, pathogens and virus.


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The fight against E.coli.
The Agri Ionization Systems, Inc. 3 step process
to prevent E.coli. Studies on Botrytis and
Phytophora included.
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1st step
Canal or well water are carriers of bacteria (E.
coli , salmonella) etc. Copper ionization has
been tested over and over with amazing results by
itself. Small amounts of an oxidizer in
conjunction with copper ions will have a even
greater log reduction. The idea is to stop the
growth in the irrigation lines (bioslimes) then
clean the canal or well water, then distribute
the copper ions to the product in the field.
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2nd Step
When using a field sprayer you want your slurry
mix to stick to the product to achieve good
results. With a sprayer unit the slurry mix
along with the positive charged copper ions,
will be attracted to the plants which are
negative. Once again the copper ions are
attacking the bacteria (E. coli, Salmonella,
Botrytis). This is the second attack.
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Step 3
When the product is taken to the packing house
and washed it should be treated with with both an
oxidizer and copper ionization to achieve the
highest log reduction possible. To avoid cross
contamination the equipment should be washed down
with no less then .5 PPMs of ionized water.
Copper ions are not affected by hot, cold, UV.
This is the third line of defense.
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www.agri-ions.com
Transplant house
Packinghouse
Field/Greenhouse irrigation
Agri Ionization Systems, Inc. will build to
suite any installation needs.
Being proactive is the key to success and safety.
Hydro Vacs
Keeping food safe is what we are all about.
Crop duster/Field sprayer
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E.coli results
STUDIES IN IONIZATION DISINFECTION Abstract
TITLE Metabolism of Escherichia coli injured by
Copper AUTHORS Domek MJ, Robbins JE, Anderson
ME, and McFeters GA PUBLICATION REF Canadian
Journal of Microbiology 33 57-62,1987 PURPOSE
OF STUDYBased on evidence that the presence of
copper in drinking water, which frequently occurs
naturally, can cause injury to Escherichia coli
(E. coli) and other coliforms, the authors
performed experiments to elucidate the
physiologic mechanisms responsible of cellular
injury. MATERIALS AND METHODSE. coli was
isolated from river water and cultured in broth.
Washed cells were placed in an inorganic carbon
buffer and the pH adjusted. High concentrations
of copper were introduced to produce injury to
the 95 level. The injured populations were
analyzed using a series of tests oxygen uptake
measurements by oxygraph and differential
respirometer, nuclear magnetic resonance
spectroscopy, gas chromatography, and
spectrophotometric respiration measurements.
RESULTSAs compared to normal cells, short-term
oxygen uptake was reduced by 75 in
copper-injured cells. Similarly, long-term
respirometry experiments showed that the rate of
oxygen uptake of the injured cells was
approximately 47 of the rate in normal
cells. Nuclear magnetic resonance spectra
revealed that the rate of glucose utilization was
64 compared to normal cells. Similar trends were
evident in lactate, ethanol, acetate, and
glutamine accumulation under both aerobic and
anaerobic conditions. The slow utilization of
succinate and decreased production of CO2 under
aerobic conditions suggested that copper has
major effect on the aerobic metabolic function of
E. coli. Copper-injured cells reduced six times
greater quantities of 2 (p-iodophenyl) -3-
(p-nitrophenyl) -5- phenyltetrazolium chloride
(INT) than control cells when NADH was used as a
substrate. A comparison of metabolic end products
demonstrated marked differences in carbon flow in
injured cells. CONCLUSIONSExposure of E. coli
in drinking water to copper produces a variety of
injurious effects. The mechanism include
decreased oxygen utilization of glucose, and
lowered accumulation of metabolic products such
as lactate and ethanol. The fact that aerobic
succinate was lowered and less glutamine produced
suggest that tricarboxylic acid (TCA) activity is
also inhibited.
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Control of bacteria and pathogens with CU
Percentage of control
ppm of copper ions
Time exposed
Number of colonies
Pathogens
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mail ionization2_at_yahoo.com Made with pride in
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