Title: Automation In Histology Advances and Improvements in Procedures, Methods, and Equipment for the Hist
1Automation In Histology Advances and
Improvements in Procedures, Methods, and
Equipment for the Histology At Massachusetts
General Hospital
- James Happel, DLM (ASCP) HTL
- Technical Director of Surgical Pathology
- Research and Development
2 Disclaimer
- This presentation is NOT intended to be the
- do all, end all
- only way to skin a cat
- we know how to do it and you dont
- all-knowing, all-seeing
- omnipotent, authoritative directive on histology.
3 Disclaimer (continued)
- This presentation IS intended to be a
- this is the way we do things
- theres more than one way to skin a cat
- many people have asked how we do things at
Massachusetts General Hospital so we thought wed
put together a Power Point for the APIII
Conference in Pittsburgh this year - presentation.
4 Disclaimer (continued)
- Therefore
- You will hear the names and models of specific
instruments and equipment used at MGH. - We are not advocating for any product/manufacturer
, these are simply the instruments/reagents/method
s we found work best for us.
5Outline
6Outline
7Outline
- MGH By The Numbers
- Where Are We Now?
8Outline
- MGH By The Numbers
- Where Are We Now?
- Instruments and Processes
9Outline
- MGH By The Numbers
- Where Are We Now?
- Instruments and Processes
- Life At Massachusetts General Hospital Pathology
10Outline
- MGH By The Numbers
- Where Are We Now?
- Instruments and Processes
- Life At Massachusetts General Hospital Pathology
- Lets Take A Tour
11Outline
- MGH By The Numbers
- Where Are We Now?
- Instruments and Processes
- Life At Massachusetts General Hospital Pathology
- Lets Take A Tour
- Questions
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14MGH Department of Pathology by the Numbers
15MGH Department of Pathology by the Numbers
16MGH Department of Pathology by the Numbers
- 800 Employees AP and CP
- Histology/Surgical Pathology Laboratory 54
Employees -
-
-
-
17MGH Department of Pathology by the Numbers
- 800 Employees AP and CP
- Histology/Surgical Pathology Laboratory 54
Employees - 26 in Histology Lab Proper (19 Histology
Technicians and Technologists) -
-
-
18MGH Department of Pathology by the Numbers
- 800 Employees AP and CP
- Histology/Surgical Pathology Laboratory 54
Employees - 26 in Histology Lab Proper (19 Histology
Technicians and Technologists) - 6 in Back Bench Accessioning (1 Laboratory
Aide) -
-
-
19MGH Department of Pathology by the Numbers
- 800 Employees AP and CP
- Histology/Surgical Pathology Laboratory 54
Employees - 26 in Histology Lab Proper (19 Histology
Technicians and Technologists) - 6 in Back Bench Accessioning (1 Laboratory
Aide) - 2 Supervisors
-
-
-
20MGH Department of Pathology by the Numbers
- 800 Employees AP and CP
- Histology/Surgical Pathology Laboratory 54
Employees - 26 in Histology Lab Proper (19 Histology
Technicians and Technologists) - 6 in Back Bench Accessioning (1 Laboratory
Aide) - 2 Supervisors
- 5 Pathologists Assistants
-
-
-
21MGH Department of Pathology by the Numbers
- 800 Employees AP and CP
- Histology/Surgical Pathology Laboratory 54
Employees - 26 in Histology Lab Proper (19 Histology
Technicians and Technologists) - 6 in Back Bench Accessioning (1 Laboratory
Aide) - 2 Supervisors
- 5 Pathologists Assistants
- 3 Grossing Technicians
-
-
22MGH Department of Pathology by the Numbers
- 800 Employees AP and CP
- Histology/Surgical Pathology Laboratory 54
Employees - 26 in Histology Lab Proper (19 Histology
Technicians and Technologists) - 6 in Back Bench Accessioning (1 Laboratory
Aide) - 2 Supervisors
- 5 Pathologists Assistants
- 3 Grossing Technicians
- 5 Transcriptionists
-
-
23MGH Department of Pathology by the Numbers
- 800 Employees AP and CP
- Histology/Surgical Pathology Laboratory 54
Employees - 26 in Histology Lab Proper (19 Histology
Technicians and Technologists) - 6 in Back Bench Accessioning (1 Laboratory
Aide) - 2 Supervisors
- 5 Pathologists Assistants
- 3 Grossing Technicians
- 5 Transcriptionists
- 5 Immunohistochemistry Technologists
-
-
24MGH Department of Pathology by the Numbers
- 800 Employees AP and CP
- Histology/Surgical Pathology Laboratory 54
Employees - 26 in Histology Lab Proper (19 Histology
Technicians and Technologists) - 6 in Back Bench Accessioning (1 Laboratory
Aide) - 2 Supervisors
- 5 Pathologists Assistants
- 3 Grossing Technicians
- 5 Transcriptionists
- 5 Immunohistochemistry Technologists
- 2 Autopsy Dieners
-
25MGH Department of Pathology by the Numbers
- Approx. 78,000 Surgical Cases/Year
26MGH Department of Pathology by the Numbers
- Approx. 78,000 Surgical Cases/Year
- 900 - 1,200 Blocks/Day Average
27MGH Department of Pathology by the Numbers
- Approx. 78,000 Surgical Cases/Year
- 900 - 1,200 Blocks/Day Average
- 2,000 HE Slides/Day Average
28MGH Department of Pathology by the Numbers
- Approx. 78,000 Surgical Cases/Year
- 900 - 1,200 Blocks/Day Average
- 2,000 HE Slides/Day Average
- 350 Immunohistochemistry Slides/Day
29MGH Department of Pathology by the Numbers
- Approx. 78,000 Surgical Cases/Year
- 900 - 1,200 Blocks/Day Average
- 2,000 HE Slides/Day Average
- 350 Immunohistochemistry Slides/Day
- 480 Autopsies/Year
30MGH Department of Pathology by the Numbers
- 80 Attending Pathologists
- 40 Residents and 10 Fellows
- Obstetrics and Gynecology
- Bone and Soft Tissue
- Gastroenterology
- Hematopathology
- Neurology
- Dermatology
- Clinical Pathology
- Nephrology
- Cytology
- Breast Pathology
- Infectious Disease
- E.N.T.
- Pulmonology
- Cardiovascular
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33October 16, 1846 Ether Dome
34October 16, 1846 Ether Dome The first
successful demonstration of ether
anesthesia
35Where Are We Now?
36What is Histology?
The ideal function of the technique of
pathological histology is to fix tissues for
microscopic examination that every tissue-element
or pathological product is perfectly preserved
with all its morphological and chemical
properties intact.
Pathological Technique
Frank Burr Mallory, A.M., M.D. and
James Homer Wright, A.M., M.D.
1904
37Histology
- Autopsy
- Cytology
- Microbiology
- Immunopathology
- - Immunohistochemistry
- - In-Situ Hybridization
- - Cytochemistry
- - Flow Cytometry/Immunofluorescence
38Work Flow Processes
- Receipt and Accession of Specimen
- Labeling of requisition and specimen container
- Printing of Cassettes
- Prosection of Specimen
- Specimen Processing (Histology Laboratory)
- Printing of Labels For Glass Slides
- Slide/Case Delivery To Pathologist
- Read and Sign Out
- Slide and Block Storage and Retention
39Histological Processes
- Tissue Processing
- Embedding
- Microtomy
- Slide drying
- Hematoxylin and Eosin Staining
- Coverslipping
40Tissue Processing
41Tissue Processing
42Tissue Processing
- Fixation
- Dehydration
- Clearing
43Tissue Processing
- Fixation
- Dehydration
- Clearing
- Infiltration
44Tissue Processing
45Tissue Processing
46Tissue Processing
- Formalin
- Alcohol
- Xylene (Chloroform)
47Tissue Processing
- Formalin
- Alcohol
- Xylene (Chloroform)
- Paraffin
48Tissue Processing Times
1904
2004
__________________________________________________
____________________
- 95 alcohol 6 hours 1. 70 alcohol 1 hour
- 100 alcohol 6 hours 2. 95 alcohol 1 hour
- Chloroform 6 hours 3. 95 alcohol 1 hour
- Chloroform w/paraffin 3 hours 4. 100
alcohol 1 hour - Paraffin bath x 2 2 hours 5. 100 alcohol 1
hour - Embed and cool quickly 6. 100 alcohol 1
hour - in cold water 7. xylene ¾ hour
- 8. xylene 1 hour
- 9. xylene 1 hour
- 10. paraffin 1 hour
- 11. paraffin 1 hour
- 12. paraffin 1 hour
- Total Processing Time 23 hours Total
Processing Time 11 ¾ hours
49Tissue Processing Times
1904
2004
__________________________________________________
____________________
- 95 alcohol 6 hours 1. 70 alcohol 1 hour
- 100 alcohol 6 hours 2. 95 alcohol 1 hour
- Chloroform 6 hours 3. 95 alcohol 1 hour
- Chloroform w/paraffin 3 hours 4. 100
alcohol 1 hour - Paraffin bath x 2 2 hours 5. 100 alcohol 1
hour - Embed and cool quickly 6. 100 alcohol 1
hour - in cold water 7. xylene ¾ hour
- 8. xylene 1 hour
- 9. xylene 1 hour
- 10. paraffin 1 hour
- 11. paraffin 1 hour
- 12. paraffin 1 hour
- Total Processing Time 23 hours Total
Processing Time 11 ¾ hours
50Throughput of Specimens
- Tissue cassettes are batched together in
baskets/racks - Approximately 150 180 cassettes per rack
- Multiple racks per processing run
- 150 540 cassettes per run
- Processing time from 8 to 11 ¾ hours
51Tissue Processors
The Autotechnicon
Ol Ironside
52Microm STP 420D
53Vision BioSystems (VBS) Peloris
54Leica Microsystems ASP 300S
55Tissue Tek VIP 5
56Histological Processes
- Tissue Processing
- Embedding
- Microtomy
- Slide drying
- Hematoxylin and Eosin Staining
- Coverslipping
57 Embedding
- - Embedding is the placing of
processed tissue specimens
into metal mold bases of liquid paraffin in
preparation for microtomy
58Embedding
59Microm EC 350
60EMS Tissuepro 150
61Tissue Tek Tec 5
62Histological Processes
- Tissue Processing
- Embedding
- Microtomy
- Slide drying
- Hematoxylin and Eosin Staining
- Coverslipping
63Microtomy and Slide Drying
- Microtomy is the sectioning of paraffin embedded
tissue specimen blocks. Routine sections cut at
4 microns - Slide Drying is the heating of slides to melt
paraffin from tissue in preparation for staining
64Microtomy and Slide Drying
65Microtomy
66Histological Processes
- Tissue Processing
- Embedding
- Microtomy
- Slide drying
- Hematoxylin and Eosin Staining
- Coverslipping
67Hematoxylin and Eosin Staining
- Hematoxylin and eosin are the life-blood of the
histology laboratory - In 2007 the MGH histology laboratory produced
approximately 400,000 slides
68Hematoxylin and Eosin Staining
1904
2004
- 1. Hematein or its ammonia salt 1
gram 1. Order pre-made hematoxylin
in gallon - 2. 90 alcohol
50 c.c. container. - 3. Alum 50 grams
2. Filter and use - 4. Water
1,000 c.c. - 5. Thymol
a crystal - Dissolve the hematein or its ammonia salt in the
- alcohol by the aid if heat, and add it to the
alum - dissolved in the water. The solution can be
- diluted with 20 parts or water or of weak alum
- solution
- Mallory and Wright
- Pathological Technique
69Hematoxylin and Eosin Staining
1904
2004
- 1. Stain 3 to 5 minutes or longer in 1.
Xylene 3 minutes - hematoxylin 2. 100 ETOH 30 seconds
- 2. Wash out in 1 alum solution until 3.
95 ETOH 15 seconds - the stain is precise 4. Running
water 15 seconds - 3. Wash thoroughly in several changes of 5.
Hematoxylin 5 minutes - water 6. Running water 15 seconds
- 4. Alcohol, 95 7. Running water 1 minute
- 5. Stain paraffin sections in a 5 to 10 8.
Acid alcohol 45 seconds - aqueous solution of eosin for 20
minutes 9. Running water 15 seconds - or longer 10. Ammonia water 5 seconds
- 6. Wash in water 11. Running water 2 minutes
- 7. Wash out a little in 90 alcohol 12. 95
ETOH 15 seconds - 8. Dehydrate in absolute alcohol 13. Eosin 45
seconds - 9. Xylol 14. 100 ETOH 30 seconds
- 10. Canada Balsam 15. 100 ETOH 30 seconds
- 16. Xylene 30 seconds
-
17.
Xylene 30 seconds - 18. Xylene 30 seconds
70Hematoxylin and Eosin Staining
1904
2004
- 1. Stain 3 to 5 minutes or longer in 1.
Xylene 3 minutes - hematoxylin 2. 100 ETOH 30 seconds
- 2. Wash out in 1 alum solution until 3.
95 ETOH 15 seconds - the stain is precise 4. Running
water 15 seconds - 3. Wash thoroughly in several changes of 5.
Hematoxylin 5 minutes - water 6. Running water 15 seconds
- 4. Alcohol, 95 7. Running water 1 minute
- 5. Stain paraffin sections in a 5 to 10 8.
Acid alcohol 45 seconds - aqueous solution of eosin for 20
minutes 9. Running water 15 seconds - or longer 10. Ammonia water 5 seconds
- 6. Wash in water 11. Running water 2 minutes
- 7. Wash out a little in 90 alcohol 12. 95
ETOH 15 seconds - 8. Dehydrate in absolute alcohol 13. Eosin 45
seconds - 9. Xylol 14. 100 ETOH 30 seconds
- 10. Canada Balsam 15. 100 ETOH 30 seconds
- 16. Xylene 30 seconds
-
17.
Xylene 30 seconds - 18. Xylene 30 seconds
71Hematoxylin and Eosin Staining
1904
2004
- 1. Stain 3 to 5 minutes or longer in 1.
Xylene 3 minutes - hematoxylin 2. 100 ETOH 30 seconds
- 2. Wash out in 1 alum solution until 3.
95 ETOH 15 seconds - the stain is precise 4. Running
water 15 seconds - 3. Wash thoroughly in several changes of 5.
Hematoxylin 5 minutes - water 6. Running water 15 seconds
- 4. Alcohol, 95 7. Running water 1 minute
- 5. Stain paraffin sections in a 5 to 10 8.
Acid alcohol 45 seconds - aqueous solution of eosin for 20
minutes 9. Running water 15 seconds - or longer 10. Ammonia water 5 seconds
- 6. Wash in water 11. Running water 2 minutes
- 7. Wash out a little in 90 alcohol 12. 95
ETOH 15 seconds - 8. Dehydrate in absolute alcohol 13. Eosin 45
seconds - 9. Xylol 14. 100 ETOH 30 seconds
- 10. Canada Balsam 15. 100 ETOH 30 seconds
- 16. Xylene 30 seconds
- 17. Xylene 30 seconds
- 18. Xylene 30 seconds
Total Staining Time 55 Minutes
Total Staining Time 18 Minutes
72Hematoxylin and Eosin Staining
1904
2004
- 1. Stain 3 to 5 minutes or longer in 1.
Xylene 3 minutes - hematoxylin 2. 100 ETOH 30 seconds
- 2. Wash out in 1 alum solution until 3.
95 ETOH 15 seconds - the stain is precise 4. Running
water 15 seconds - 3. Wash thoroughly in several changes of 5.
Hematoxylin 5 minutes - water 6. Running water 15 seconds
- 4. Alcohol, 95 7. Running water 1 minute
- 5. Stain paraffin sections in a 5 to 10 8.
Acid alcohol 45 seconds - aqueous solution of eosin for 20
minutes 9. Running water 15 seconds - or longer 10. Ammonia water 5 seconds
- 6. Wash in water 11. Running water 2 minutes
- 7. Wash out a little in 90 alcohol 12. 95
ETOH 15 seconds - 8. Dehydrate in absolute alcohol 13. Eosin 45
seconds - 9. Xylol 14. 100 ETOH 30 seconds
- 10. Canada Balsam 15. 100 ETOH 30 seconds
- 16. Xylene 30 seconds
-
17. Xylene 30 seconds - 18. Xylene 30 seconds
Total Staining Time 55 Minutes
Total Staining Time 18 Minutes
73Hematoxylin and Eosin Staining
74Thermo Scientific Shandon Varistain Gemini ES
75Leica XL Slide Stainer
76Leica ST4040 Linear Stainer
77Histological Processes
- Tissue Processing
- Embedding
- Microtomy
- Slide drying
- Hematoxylin and Eosin Staining
- Coverslipping
78Coverslipping
79Leica CV 5030 Coverslipper
80Tissue Tek Glas Coverslipper
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82Tissue Tek Primsa and Film
83Microwave Processing
84Microwave Processing
- Uses precise microwave energy to process tissue
specimens - Utilizes standard processing reagents formalin,
alcohol, xylene and paraffin - Allows for continuous throughput of specimens
85Microwave Processing
Routine Processing
Routine processing uses formalin, alcohol, xylene
and paraffin for specimens sectioned to a
thickness of up to 5 mm Reprocessing is performed
using the same programmed times as routine
processing
Vs.
Features
Number of
Cassettes 150
Histoprocessing time
Specimen thickness
180 minutes
needle biopsies
240 480 minutes
lt 3 mm
480 660 minutes
lt 5 mm
86Microwave Processing
Routine Processing
Routine processing uses formalin, alcohol, xylene
and paraffin for specimens sectioned to a
thickness of up to 5 mm Reprocessing is performed
using the same programmed times as routine
processing
Features
Number of
Cassettes 150
Histoprocessing time
Specimen thickness
180 minutes
needle biopsies
240 480 minutes
lt 3 mm
480 660 minutes
lt 5 mm
87Microwave Processing
Routine Processing
Routine processing uses formalin, alcohol, xylene
and paraffin for specimens sectioned to a
thickness of up to 5 mm Reprocessing is performed
using the same programmed times as routine
processing
Features
Number of
Cassettes 150
Histoprocessing time
Specimen thickness
180 minutes
needle biopsies
240 480 minutes
lt 3 mm
480 660 minutes
lt 5 mm
88Microwave Processing
Routine Processing
Routine processing uses formalin, alcohol, xylene
and paraffin for specimens sectioned to a
thickness of up to 5 mm Reprocessing is performed
using the same programmed times as routine
processing
Features
Number of
Cassettes 150
Histoprocessing time
Specimen thickness
180 minutes
needle biopsies
240 480 minutes
lt 3 mm
480 660 minutes
lt 5 mm
89Microwave Processing
Routine Processing
Routine processing uses formalin, alcohol, xylene
and paraffin for specimens sectioned to a
thickness of up to 5 mm Reprocessing is performed
using the same programmed times as routine
processing
Features
Number of
Cassettes 150
Histoprocessing time
Specimen thickness
180 minutes
needle biopsies
240 480 minutes
lt 3 mm
480 660 minutes
lt 5 mm
90Microwave Processing
Routine Processing
Routine processing uses formalin, alcohol, xylene
and paraffin for specimens sectioned to a
thickness of up to 5 mm Reprocessing is performed
using the same programmed times as routine
processing
Features
Number of
Cassettes 150
Histoprocessing time
Specimen thickness
180 minutes
needle biopsies
240 480 minutes
lt 3 mm
480 660 minutes
lt 5 mm
91Microwave Processing
Routine Processing
Routine processing uses formalin, alcohol, xylene
and paraffin for specimens sectioned to a
thickness of up to 5 mm Reprocessing is performed
using the same programmed times as routine
processing
Features
Number of
Cassettes 150
Histoprocessing time
Specimen thickness
180 minutes
needle biopsies
240 480 minutes
lt 3 mm
480 660 minutes
lt 5 mm
92Microwave Processing
Routine Processing
Routine processing uses formalin, alcohol, xylene
and paraffin for specimens sectioned to a
thickness of up to 5 mm Reprocessing is performed
using the same programmed times as routine
processing
Features
Number of
Cassettes 150
Histoprocessing time
Specimen thickness
180 minutes
needle biopsies
240 480 minutes
lt 3 mm
480 660 minutes
lt 5 mm
93Microwave Processing
Routine Processing
Routine processing uses formalin, alcohol, xylene
and paraffin for specimens sectioned to a
thickness of up to 5 mm Reprocessing is performed
using the same programmed times as routine
processing
Features
Number of
Cassettes 150
Histoprocessing time
Specimen thickness
180 minutes
needle biopsies
240 480 minutes
lt 3 mm
480 660 minutes
lt 5 mm
94Microwave Processing
Routine Processing
Routine processing uses formalin, alcohol, xylene
and paraffin for specimens sectioned to a
thickness of up to 5 mm Reprocessing is performed
using the same programmed times as routine
processing
Features
Number of
Cassettes 150
Histoprocessing time
Specimen thickness
180 minutes
needle biopsies
240 480 minutes
lt 3 mm
480 660 minutes
lt 5 mm
95Microwave Processing
Routine Processing
Routine processing uses formalin, alcohol, xylene
and paraffin for specimens sectioned to a
thickness of up to 5 mm Reprocessing is performed
using the same programmed times as routine
processing
Features
Number of
Cassettes 150
Histoprocessing time
Specimen thickness
180 minutes
needle biopsies
240 480 minutes
lt 3 mm
480 660 minutes
lt 5 mm
96Microwave Processing
- Microwave systems allow for continuous
throughput - Less flexibility due to greater specificity of
function
97Keys to Microwave Processing
98Keys to Microwave Processing
99Keys to Microwave Processing
100Keys to Microwave Processing
101Keys to Microwave Processing
102Keys to Microwave Processing
103Pelco BioWave Pro
104Milestone Pathos Microwave Processor
105Hacker MARS Microwave Histoprocessor
106Rapid Processing
107Rapid Processing
- Reduces processing time from 8 11 ¾ hours to
2.5 6 hours
108Rapid Processing
- Reduces processing time from 8 11 ¾ hours to
2.5 6 hours - Rapid processing utilizes
methanol, ethanol and isopropanol No clearing
agents (xylene) used. This procedure does have a
tendency to dry out (albeit slightly) smaller
biopsies due the abundant alcohol.
109Rapid Processing/Microwave
110Rapid Processing/Microwave
Sakura Fineteks Tissue-Tek Xpress
111Rapid Processing/Microwave
Sakura Fineteks Tissue-Tek Xpress
112Rapid Processing/Microwave
Sakura Fineteks Tissue-Tek Xpress
113Rapid Processing/Microwave
114Rapid Processing/Microwave
115Rapid Processing/Microwave
116Rapid Processing/Microwave
117Rapid Processing/Microwave
118Rapid Processing/Microwave
- Allows for continuous throughput of specimens
119Embedding
120Embedding
121Tissue Tek Paraform Sectionable Cassette System
122Tissue Tek Paraform Sectionable Cassette System
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128Tissue Tek Paraform Sectionable Cassette System
Sakura Fineteks Tissue-Tek Autotek
- Continuous throughput of specimens
129Tissue Tek Paraform Sectionable Cassette System
- Continuous throughput of specimens
130Hematoxylin and Eosin Staining
- Ready to use Hematoxylin and Eosin Staining
System
SurgiPath Selec Tech
131Hematoxylin and Eosin Staining
- High Definition HE
Ventana Medical Systems, Inc.
132Hematoxylin and Eosin Staining
- Ventana Medcial systems, Inc.
133Hematoxylin and Eosin Staining
- Modular system xylene free
- Slides input directly from waterbath
- Dries, stains and coverslips slide with bar
coded label - Continuous throughput
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135Life At Massachusetts General Hospital Pathology
136Life At Massachusetts General Hospital Pathology
- Life at MGHs surgical pathology laboratory
starts with our Laboratory Information System
137Life At Massachusetts General Hospital Pathology
- Several years ago, MGH began using the Tamtron
PowerPath LIS
138Life At Massachusetts General Hospital Pathology
- Several years ago, MGH began using the Tamtron
PowerPath LIS - Dr. Ulysses Balis started tinkering with
PowerPath to customize it for use in the MGH
surgical pathology laboratory
139Life At Massachusetts General Hospital Pathology
- Several years ago, MGH began using the Tamtron
PowerPath LIS - Dr. Ulysses Balis started tinkering with
PowerPath to customize it for use in the MGH
surgical pathology laboratory - Dr. Balis created AP Middleware
140Life At Massachusetts General Hospital Pathology
- Several years ago, MGH began using the Tamtron
PowerPath LIS - Dr. Ulysses Balis started tinkering with
PowerPath to customize it for use in the MGH
surgical pathology laboratory - Dr. Balis created AP Middleware
- The finished product is known today as
PowerPaths AMP Module
141Life At Massachusetts General Hospital Pathology
- Using the LEAN principles of
- Just in time supply
- Right person right job
- Work flow continuity up-stream processes in
direct proximity to down-stream processes
142Histology LEAN Project
143Life At Massachusetts General Hospital Pathology
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145Life At Massachusetts General Hospital Pathology
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147OR Window
Specimen Accessioning
Specimen Accessioning
Specimen Accessioning
Cassetting
Telepathology Scope
Cryo
Scope
FS
Scope
Scope
FS
Saw Room
PA
Photography
PA
Cryo
Storage
HE Staining
Storage
Lung CV
Storage
Storage
ENT
GIL
BRST
BST
GU
GYL
OB
148OR Window
Specimen Accessioning
Specimen Accessioning
Specimen Accessioning
Cassetting
Telepathology Scope
Cryo
Scope
FS
Scope
Scope
FS
Saw Room
PA
Photography
PA
Cryo
Storage
HE Staining
Storage
Lung CV
Storage
Storage
ENT
GIL
BRST
BST
GU
GYL
OB
149OR Window
Specimen Accessioning
Specimen Accessioning
Specimen Accessioning
Cassetting
Telepathology Scope
Cryo
Scope
FS
Scope
Scope
FS
Saw Room
PA
Photography
PA
Cryo
Storage
HE Staining
Storage
Lung CV
Storage
Storage
ENT
GIL
BRST
BST
GU
GYL
OB
150OR Window
Specimen Accessioning
Specimen Accessioning
Specimen Accessioning
Cassetting
Telepathology Scope
Cryo
Scope
FS
Scope
Scope
FS
Saw Room
PA
Photography
PA
Cryo
Storage
HE Staining
Storage
Lung CV
Storage
Storage
ENT
GIL
BRST
BST
GU
GYL
OB
151OR Window
Specimen Accessioning
Specimen Accessioning
Specimen Accessioning
Cassetting
Telepathology Scope
Cryo
Scope
FS
Scope
Scope
FS
Saw Room
PA
Photography
PA
Cryo
Storage
HE Staining
Storage
Lung CV
Storage
Storage
ENT
GIL
BRST
BST
GU
GYL
OB
152OR Window
Specimen Accessioning
Specimen Accessioning
Specimen Accessioning
Cassetting
Telepathology Scope
Cryo
Scope
FS
Scope
Scope
FS
Saw Room
PA
Photography
PA
Cryo
Storage
HE Staining
Storage
Lung CV
Storage
Storage
ENT
GIL
BRST
BST
GU
GYL
OB
153OR Window
Specimen Accessioning
Specimen Accessioning
Specimen Accessioning
Cassetting
Telepathology Scope
Cryo
Scope
30 seconds to walk
FS
Scope
Scope
FS
Saw Room
PA
Photography
PA
Cryo
Storage
HE Staining
Lung CV
Storage
Storage
Storage
ENT
GIL
BRST
BST
GU
GYL
OB
154OR Window
Specimen Accessioning
Specimen Accessioning
Specimen Accessioning
Cassetting
Telepathology Scope
Cryo
Scope
Between 250 to 300 cases/day
FS
Scope
Scope
FS
Saw Room
PA
Photography
PA
Cryo
Storage
HE Staining
Lung CV
Storage
Storage
Storage
ENT
GIL
BRST
BST
GU
GYL
OB
155OR Window
Specimen Accessioning
Specimen Accessioning
Specimen Accessioning
Cassetting
Telepathology Scope
Cryo
Scope
Just over 2 hours/day walking across the lab
FS
Scope
Scope
FS
Saw Room
PA
Photography
PA
Cryo
Storage
HE Staining
Lung CV
Storage
Storage
Storage
ENT
GIL
BRST
BST
GU
GYL
OB
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171Specimen Accessioning
Specimen Accessioning
Specimen Accessioning
Cassetting
Telepathology Scope
Cryo
Scope
FS
Scope
Scope
FS
Saw Room
PA
Photography
PA
Cryo
Storage
HE Staining
Storage
Lung CV
Storage
Storage
ENT
GIL
BRST
BST
GU
GYL
OB
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183POWERPATH Cassette LabelGeneral Data Laser
Printed
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187OR Window
Specimen Accessioning
Specimen Accessioning
Specimen Accessioning
Cassetting
Telepathology Scope
Cryo
Scope
FS
Scope
Scope
FS
Saw Room
PA
Photography
PA
Cryo
Storage
HE Staining
Storage
Lung CV
Storage
Storage
ENT
GIL
BRST
BST
GU
GYL
OB
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194Small Grossing Technicians
Storage
Tech 2
Tech 1
Cassetting
Storage
Supervisors Office
Supervisors Office
Tech 4
Tech 3
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199Storage
7
Embedding
6
5
8 Tissue Processors
4
MW
3
2
1
My Office
Pod 1
HE Stainers
Cover Slippers
Pod 2
Embed
and
Pod 3
Embed
Pod 4
Block and Slide Reconciliation
Slide Checkout
Special Stains
Storage
Immuno Cutting
Neuro Path
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206Storage
7
Embedding
6
5
8 Tissue Processors
4
MW
3
2
1
My Office
Pod 1
HE Stainers
Cover Slippers
Pod 2
Embed
and
Pod 3
Embed
Pod 4
Block and Slide Reconciliation
Slide Checkout
Special Stains
Storage
Immuno Cutting
Neuro Path
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218Whats Being Worked On At MGH?
219Automated Microtomy
220Automated Microtomy
- Kurabo Industries, LTD. Osaka, Japan developing
the AS-200 Research application
221 High voltage power supply for positive
charge
Carrier tape
Slide glass
Adhering solution
Peltier-Element (Cooling)
Electrode
Heating (Stretching)
Tissue block
Blade
Humidification
Humidification
Condensation
Heating (stretching and drying)
222Sections Cut From AS-200
223Thicker-gtDarker
9um
2um
224Whole Slide Imaging (WSI)
225Whole Slide Imaging (WSI)
- The area of tissue is ltlt than the area of the
slide - A low resolution, wide angle camera takes an
snapshot of the entire slide - Proprietary algorithms use that image identify
the area that is tissue (as opposed to glass,
dirt and other artifacts) - The system will then only image the areas of
tissue
Dirt
Edge of cover slip
S06-1000 A 2 1 HE Patrick Patient
Tissue
226Whole Slide Imaging (WSI)
- Tissue Finding Algorithms
- Tissue finding can significantly increase capture
speed by limiting the amount of area the needs to
captured - But tissue finding is difficult and potentially
catastrophic if an area of tissue is not
identified
Dirt
Edge of cover slip
S06-1000 A 2 1 HE Patrick Patient
Tissue
227Whole Slide Imaging (WSI)
- Pre-focusing
- High speed system do not stop and focus at each
new tile - Using the low resolution, wide field image, the
system choses 10 80 points - They perform traditional auto-focusing on those
areas - The system then knows exactly what the working
distance needs to be to be in focus at those
points
228Whole Slide Imaging (WSI)
- Pre-focusing
- During image capture, the objective lens flies
along the estimated focus surface above the slide
within the area identified as tissue - As the camera flies, it captures tiles or
strips that are then knitted together to form
the complete whole slide image - A precise stage and precise communication between
the stage and the camera is the key in making
this work
S06-1000 A 2 1 HE Patrick Patient
Estimate Focus Surface
Focus Point Tissue Focus Point
229A Slightly Different Approach
Array Microscopy
230A Slightly Different Approach
- Rather than using a single lens to view an image
on a slide, use 80 lenses to view the same area.
VS
231A Slightly Different Approach
Used with permission from Michael R. Descour,
Ph.D., President , DMetrix
232A Slightly Different Approach
Used with permission from Michael R. Descour,
Ph.D., President , DMetrix
233Multi-Spectral Imaging
234Multi-Spectral Imaging
- Components of a tissue section transmit and
absorb light at a variety of different
wavelengths. This pattern is specific for each
individual component. - In multi-spectral imaging, images are taken at
discrete wavelengths and identify tissue
components by the way those specific wavelengths
are absorbed (or transmitted) A fingerprint,
so to speak.
235Multi-Spectral Imaging
Glass Slide
Tissue
Camera
Light Source
Server
16 Band Filter
236Multi-Spectral Imaging
- What does this enable us to do?
237Multi-Spectral Imaging
- What does this enable us to do?
- Virtual Slide Staining
238Digital Adjustment
239Digital Adjustment
240Digital Adjustment
241Digital Adjustment
242Digital Adjustment
243Digital Adjustment
244Digital Adjustment
245Digital Adjustment
246Digital Adjustment
247Physical HE Stain
248Physical HE Stain
Digital Trichrome Stain From HE stain
249Physical Trichrome Stain
Digital Trichrome Stain From HE stain
250Specimen Discard
- Using the PowerPath LIS
- Time of Case Sign Out is Recorded
- 14 Days After Sign Out Bar Code Labeled Are
Scanned - If the Specimen Can Be Discarded
251Specimen Discard
252Specimen Discard
- Using the PowerPath LIS
- Time of Case Sign Out is Recorded
- 14 Days After Sign Out Bar Code Labeled Are
Scanned - If the Specimen Can Be Discarded
- If the Specimen Should Not Be Discarded
253Specimen Discard
254Whats Next?
255Whats Next?
- Radiofrequency Identification (RFID)
256Whats Next?
- Radiofrequency Identification (RFID)
- Telepathology
257Whats Next?
- Radiofrequency Identification (RFID)
- Telepathology
- Digital Slide Imaging - Whole Slide Imaging
258Whats Next?
- Radiofrequency Identification (RFID)
- Telepathology
- Digital Slide Imaging - Whole Slide Imaging
- Virtual Slide Staining
259Whats Next?
- Radiofrequency Identification (RFID)
- Telepathology
- Digital Slide Imaging - Whole Slide Imaging
- Virtual Slide Staining
- Completely Automated Histology Laboratories
260Lots and Lots of Data on Every Slide
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262Special Thanks to John Gilbertson Yukako
Yagi Ulysses Balis Bruce Hamaty David
Wilbur Anand Dighe Michael Descour Andrew
Rosenberg Steve Conley Michelle Lee Walter Van
Tilburg Jim Bacho Babiker Bedri Paul Weber
263Questions?
Thank You James Happel