Stem Cell Therapy

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Stem Cell Therapy Cardiovascular Molecular
Imaging

• Joseph Wu, MD, PhD
• Department of Medicine/Cardiology
• Department of Radiology/Nuclear Medicine
• Email joewu_at_stanford.edu

MIPS
Stanford
School of Medicine Department of Radiology
Molecular Imaging Program at Stanford
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Background

• Coronary heart disease is the 1 cause of
  morbidity and mortality in the US.
• CHF is the 1 cause of hospitalization for those
  age gt65 yo.
• Annual health care costs related to
  cardiovascular diseases was 220 billion last
  year.
• Stem cell transplant is a promising and exciting
  therapy.

Orlic D, et al. Nature 2001
2004 American Heart Association Update
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Available Stem Cells

• Bone marrow stem cells (BMSC)
• Endothelial progenitor cells (EPC)
• Mesenchymal stem cells (MSC)
• Skeletal myoblasts (SKM)
• Embryonic stem cells (ESC)
• Cardiac stem cells (CSC)
• Cardiac progenitor cells (isl1)

Stem cells are capable of self-renewal,
transformation into dedicated progenitor cells,
and differentiation into specialized progeny
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Imaging Embryonic Cardiomyoblasts Survival
Optical Imaging
MicroPET Imaging
Wu JC et al, Circulation 2003
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Imaging Bone Marrow Stem Cells Engraftment in
Living Subjects
Ahmad Sheikh
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PET Imaging of ESC Transplant
1ID/g
horizontal
coronal
sagittal
18F-FHBG
0ID/g
3ID/g
18F-FDG
0ID/g
Fusion
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PET Imaging of Teratoma Formation and Selective
Ablation by GCV Treatment
Feng Cao
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Imaging ES Cell Differentiation into
Cardiomyocytes
Nkx 2.5-eGFP 12 days after embryoid body
differentiation
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Other Projects

• Comparison of BMMNC vs MSC vs HSC
• Imaging of stem cell migration homing
• Tissue engineering based on ESC
• EPC and diabetic cardiomyopathy
• Isolation of resident cardiac stem cells
• SiRNA knockdown of gene expression