MODELING TEAM

1
MODELING TEAM REPORT July 28, 2009 Mary
Leon
2
CONTENTS
3
1. Modeling Plan for SiGEM
4
Modeling Team Subprojects
5
2. Further Analysis on the IDO Enzyme Model
6
The Reaction 5MT--Trp
REACTION 1L-tryptophan O2 N-formyl-L-kynureni
ne
E
REACTION 2 5-methyl-tryptophan O2
4-(2-amino-5-methylphenyl)-2-(formylamino)-4-oxob
utanoic acid
7
Observations
8
Observations
9
Observations
10
An Explanation with Equations
11
Speeding Up the Reaction
S0 10mM
S0 2.28e-6mM
12
3. Mutated and Regular Trp-Repressor
13
The Reaction Trp/5MT?DNA-P/O
Input
Output
14
Equations
15
Observation Trp00.01M
16
Observation Trp00.001M
17
The Reaction 5MT?IL-6
18
Observation 5MT-gtIL-6
Level of il-6 over time
IL-6 Export?
19
4. The Production of B-carotene with IPP
20
The Reaction 8IPP-gtB-Carotene
21
5. BLH Enzyme Kinetics
22
The Reaction B-Carotene?Retinoic Acid
23
Observations
B-carotene input? BLH levels?
24

• Thanks for your attention