Outline of the seminar

1
Outline of the seminar
1999/10/27 ?? ??? ???? ???
Probing cell-surface architecture through
synthesis An NMR-determined structural motif for
tumor-associated mucins. PNAS(1999)963489-3493

• BACKGROUND
• Mucin-like glycoprotein
• N-linked/O-linked glycosylation
• located in ECM (extracellular matrix)
• altered expression in malignant cells (e.g.
  pancreatic cancer, breast carcinoma, etc.)
• Leukosialin, CD43
• its function
• T cell antigen-specific activation enhancement
• X-linked Wiskott-Aldrich syndrome with
  sialophorin defect
• its structure
• EM visualization
• cDNA sequence (human chromosome 16p11.2) 341
  a.a.
• extracellular 195 a.a.
• hydrophobic transmembrane 23 a.a.
• C-terminal intracellular 123 a.a.
• EXPERIMENT
• 1D 1H NMR spectra
• conformational change induced by a-linked isomer
• NH exchange lifetime