SEGMENTAL RESPONSE OF THE MACROPHAGE PLASMA MEMBRANE TO A PHAGOCYTIC STIMULUS* BY FRANK M. GRIFFIN, JR., AND SAMUEL C. SILVERSTEIN (From The Rockefeller University, New York 10021) (Received for publication 22 October 1973)

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SEGMENTAL RESPONSE OF THE MACROPHAGE
PLASMAMEMBRANE TO A PHAGOCYTIC STIMULUSBY
FRANK M. GRIFFIN, JR., AND SAMUEL C.
SILVERSTEIN(From The Rockefeller University,
New York 10021)(Received for publication 22
October 1973)

• Presented by Akihiro Tsuyada

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Abstract (1)

• There is evidence that ingestion of one type of
  particle enhances the rate of ingestion of a
  different type of particle.
• However, it is not sure whether stimulated
  phagocytic response of Macrophage plasma membrane
  uptakes particles selectively or not.

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Abstract (2)

• Prepared immunologically attached mouse RBCs to
  macrophages (Mf), using the F(ab)2 fragments of
  rabbit and sheep immunoglobulins.
• Mfs ingested the Mfplasma membrane attached RBCs
  when rabbit anti-mouse RBC IgG is added
• Mf-RBCs ingested particles by phagocytosis, but
  not ingested RBCs on the Mf plasma membrane.
• Ingestion of one particle does not trigger
  generalized phagocytosis of all particles
  attached to the Mf plasma membrane.

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Result and Discussion (1-1)

• Hemagglutination titers of F(ab)2 Ig Fragments
  and of rabbit anti-mouse RBC IgG (Table1)
• - Rabbit anti-mouse (RAM) RBC IgG agglutinated
  mouse RBCs
• - RAM-Mf F(ab)2 agglutinated mouse RBCs
• - At least one membrane antigen is shared
  between RBCs and Mf

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Result and Discussion (1-2)

• - RAM-Mf F(ab)2 coated mouse RBC
• RAM-RBC IgG ? no agglutination
• RAM-Mf F(ab)2 ? no agglutination
• SAR F(ab)2 ? agglutination

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Result and Discussion (2-1)

• Immunological attachment of RBCs to Mf
• - RAM-Mf F(ab)2 coated mouse RBC and RAM-Mf
  F(ab)2 coated mouse Mf linked by SAR F(ab)2

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Result and Discussion (2-2)

• - 98 of Mf attached an average of 11 RBCs per
  cell. (Table2 and Fig1-a)
• - Employed throughout the remaining experiments
  to attach RBCs to Mf.

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Result and Discussion (3-1)

• The effect of RAM-Mf F(ab)2 coated mouse Mf on
  the phagocytosis of latex particles and opsonized
  pnumococci.

?
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Result and Discussion (3-2)

• - RAM-Mf F(ab)2 coated mouse Mf did not affect
  the phagocytosis of latex and opsonized
  pnumococci.

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Result and Discussion (4-1)

• The effect of test particle ingestion on the
  immunologically attached RBCs and Mf

?
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Result and Discussion (4-2)
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Result and Discussion (4-3)

• - The ingestion of test particles by RAM-Mf
  F(ab)2 coated mouse Mf and the exclusion of
  RAM-Mf F(ab)2 coated mouse RBCs
• - The Mf test particle ingestion did not induce
  the ingestion of RAM-Mf F(ab)2 coated mouse RBCs
  on the Mf plasma membrane.

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Result and Discussion (5-1)

• Effect of RAM RBC IgG on the ingestion by Mf of
  immunologically attached RBCs

?
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Result and Discussion (5-2)
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Result and Discussion (5-3)

• - More than 98 of the Mf ingested an average of
  seven RBCs/ Mf
• - Not proven whether ingested RBCs are attached
  RBCs or floated RAM-Mf F(ab)2 coated RBCs.

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Result and Discussion (5-4)

• Upside down experiment
• - Place the slide grass upside down for a
  gravity to pull free floating RBCs down.
• - Plasma membrane attached RBCs were ingested by
  Mf RAM RBC IgG bound to immunologically attached
  RBCs and then it triggered that Mf ingested RBCs.

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Result and Discussion (6-1)

• The effect of colchicine on the capacity of the
  Mf to selectivity ingest portion of its plasma
  membrane during phagocytosis.
• - Colchicine may cause disorganization, or
  random distribution of cell surface components.
• - Colchicine did not affect on the
  immunologically attached RBCs-Mf complexes.
• - No affect on test particle ingestion.

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Conclusion

• Ingestion of one particle does not trigger
  generalized phagocytosis of all particles
  attached to the Mf plasma membrane.

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