FIP: an automated beamline for protein crystallography

1
G-Rob 1D
G-Rob 1D replaces both the changing device and
the goniometer, It also enables to collect
data along any chosen axis, It was designed to
handle the classical cryo-cooled samples
(ESRF/EMBL standard), as well as several other
standards.
G-Rob, in the G-Rob 1D configuration, is
handling frozen crystals for crystallography data
collection
Lab. Setup (includes also the plate screening)
G-Rob transfering samples of different formats
from a storage Dewar to the X-Ray beam
G-Rob collecting data with any orientation of the
spindle axis, for a better strategy of data
collection
The G-Rob systems are produced by NatX-ray
(www.natx-ray.com) and distributed by IRELEC
(www.irelec.com)
2
An example of achievements using G-Rob 1D
Comparison of data collected on FIP-B30A with the
goniometer
G-Rob 1D
resolution 30-1.9 Å (2.0-1.9 Å) completness
98.2 (97.3) I/?(I) 16 (7.3) Rsym 6.0 (16.9)
resolution 30-1.9 Å (2.0-1.9 Å) completness
96.8 (96.9) I/?(I) 12 (3.8) Rsym 9.8 (37.2)
Data were collected on the same lysozyme crystal
(but not necessarily with the same orientation).
Space group is p43212. Values between brackets
are for the last resolution shell.
Sphere of confusion 12 micron diam. gt OK for
labs and BM beamlines

• Questions
• What is the best strategy for Phi data collection
• Is there a software to help for that?
• What are the best software for data reduction in
  this case
• Etc