Optical and Atomic Force Microscopy for analysis of wet sediments from the Canale dei Navicelli - PowerPoint PPT Presentation

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Optical and Atomic Force Microscopy for analysis of wet sediments from the Canale dei Navicelli

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Title: Optical and Atomic Force Microscopy for analysis of sediments from the Canale dei Navicelli Author: Antlab Last modified by: Antlab Created Date – PowerPoint PPT presentation

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Title: Optical and Atomic Force Microscopy for analysis of wet sediments from the Canale dei Navicelli


1
Optical and Atomic Force Microscopy for analysis
of wet sediments from the Canale dei Navicelli
LIFE 12 ENV IT 000652 Cleansed
2
Two methods
  • Analysis with Optical Microscopy (shape, size,
    granulometry)
  • Analysis with scanning probe by AFM (Atomic Force
    Microscope)

3
Optical Microscopy
  • Biological microscope with high-resolution (16
    Mpixel) reflex on trinocular tube for digital
    imaging
  • Software
  • ImageJ (developed by the National Institute of
    Health) for quantitative analysis (real
    dimensions-pixel, particle counting)
  • Helicon Focus Pro for image stacking (to
    increase the depth of field)

4
AFM Microscopy
  • Nanosurf Easyscan 2 Microscope
  • Scanning head 110110 micron
  • Maximum Z range 22 micron
  • Maximum resolution 20482048 pixel

5
Granulometry
  • The texture has great importance for the physical
    and biological properties of the soil
  • Classification (USDA)
  • clay, diameter less than 2 micron
  • silt, diameter between 2 and 50 micron
  • sand, between 50 micron and 2 mm.

6
Sample 1
Total counting 4002
Sample 3
Total counting 2005
7
Sample 5
Total counting 2013
Sample 7
Total counting 3442
8
Sample 1
Cyclotella Meneghiniana (MC)
Cyclotella Meneghiniana (Moder. Cont.)
Staurastrum anatinum (MC)

9
Sample 1
Cyclotella Meneghiniana (MC)
Melosira Varians (MC)
Melosira Moniliformis (MC)
Navicula Rhyncotella (MC)
10
Sample 1
Cyclotella Meneghiniana (MC)
Melosira Moniliformis (MC)
Cyclotella Meneghiniana (MC)
Bacillaria Paxillifer (MC to HC)
11
Sample 1
AFM measurements of sedimentary rock composed by
centric
12
Sample 1
AFM trace of diatom (Nitzschia Acicularis (MC to
HC))
13
Sample 3
Surirella Brebissonii (MC)
Nitschia Sublinearis (MC to HC)
Gyrosigma Nodiferum (MC)
14
Sample 3
AFM trace of diatom (Phase contrast)
15
Sample 3
AFM trace of diatom
16
Sample 3
AFM trace of diatom
Zoom at 45 micron
17
Sample 5
18
Sample 7
Fragilaria Acus (SC)
Fragilaria Acus (SC)
19
Sample 7
In some cases the AFM cantilever can displace a
diatom
Before
After
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