Title: Optical and Atomic Force Microscopy for analysis of wet sediments from the Canale dei Navicelli
1Optical and Atomic Force Microscopy for analysis
of wet sediments from the Canale dei Navicelli
LIFE 12 ENV IT 000652 Cleansed
2Two methods
- Analysis with Optical Microscopy (shape, size,
granulometry) - Analysis with scanning probe by AFM (Atomic Force
Microscope)
3Optical Microscopy
- Biological microscope with high-resolution (16
Mpixel) reflex on trinocular tube for digital
imaging - Software
- ImageJ (developed by the National Institute of
Health) for quantitative analysis (real
dimensions-pixel, particle counting) - Helicon Focus Pro for image stacking (to
increase the depth of field)
4AFM Microscopy
- Nanosurf Easyscan 2 Microscope
- Scanning head 110110 micron
- Maximum Z range 22 micron
- Maximum resolution 20482048 pixel
5Granulometry
- The texture has great importance for the physical
and biological properties of the soil - Classification (USDA)
- clay, diameter less than 2 micron
- silt, diameter between 2 and 50 micron
- sand, between 50 micron and 2 mm.
6Sample 1
Total counting 4002
Sample 3
Total counting 2005
7Sample 5
Total counting 2013
Sample 7
Total counting 3442
8Sample 1
Cyclotella Meneghiniana (MC)
Cyclotella Meneghiniana (Moder. Cont.)
Staurastrum anatinum (MC)
9Sample 1
Cyclotella Meneghiniana (MC)
Melosira Varians (MC)
Melosira Moniliformis (MC)
Navicula Rhyncotella (MC)
10Sample 1
Cyclotella Meneghiniana (MC)
Melosira Moniliformis (MC)
Cyclotella Meneghiniana (MC)
Bacillaria Paxillifer (MC to HC)
11Sample 1
AFM measurements of sedimentary rock composed by
centric
12Sample 1
AFM trace of diatom (Nitzschia Acicularis (MC to
HC))
13Sample 3
Surirella Brebissonii (MC)
Nitschia Sublinearis (MC to HC)
Gyrosigma Nodiferum (MC)
14Sample 3
AFM trace of diatom (Phase contrast)
15Sample 3
AFM trace of diatom
16Sample 3
AFM trace of diatom
Zoom at 45 micron
17Sample 5
18Sample 7
Fragilaria Acus (SC)
Fragilaria Acus (SC)
19Sample 7
In some cases the AFM cantilever can displace a
diatom
Before
After