A%20G%20C%20C%20G%20A%20T%20G%20T%20C%20G%20T%20C%20G%20T%20C%20T%20A%20C%20C%20A%20T%20G%20C%20A%20T - PowerPoint PPT Presentation
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A%20G%20C%20C%20G%20A%20T%20G%20T%20C%20G%20T%20C%20G%20T%20C%20T%20A%20C%20C%20A%20T%20G%20C%20A%20T
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Title: No Slide Title Author: Mike Zeller Last modified by: Protein Facility Created Date: 5/1/2002 3:45:17 PM Document presentation format: On-screen Show – PowerPoint PPT presentation
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Title: A%20G%20C%20C%20G%20A%20T%20G%20T%20C%20G%20T%20C%20G%20T%20C%20T%20A%20C%20C%20A%20T%20G%20C%20A%20T
1
Polymerase Chain Reaction (PCR)
Part I
Segment of Interest
A G C C G A T G T C G T C G T C T
A C C A T G C A T
2
Part II
C C G A T
A T G G T
3
Part III
C C G A T G T C G T
A G C A G A T G G T
4
Part IV
C C G A T G T C G T
A G C A G A T G G T
5
Part IV
C C G A T G T C G T
G G C T A C A G C A G C A G A T G G T
A G C A G A T G G T
C C G A T G T C G T C G T C T A C C A
6
Part IV
C C G A T G T C G T
G G C T A C A G C A G C A G A T G G T
A G C A G A T G G T
C C G A T G T C G T C G T C T A C C A
1
1
2
2
1
1
7
Part V
3 T C G G C T A C A G C A G C A G A T G G T A C
G T A 5
C1
5 C C G AT G T C G T C G T C T AC C A T G C
A T 3
C C G A T G T C G T C G T C T A C C A T G C A T
CC1
C C G A T G T C G T C G T C T A C C A T G C A T
CC1
5 A G C C G A T G T C G T C G T C T A C C A T G
C A T 3
C2
T C G G C T A C A G C A G C AG A T G G T
T C G G C T A C A G C A G C AG A T G G T
CC2
T C G G C T A C A G C A G C AG A T G G T
CC2
8
Part V
3 T C G G C T A C A G C A G C A G A T G G T A C
G T A 5
C1
5 C C G AT G T C G T C G T C T AC C A T G C
A T 3
C C G A T G T C G T C G T C T A C C A T G C A T
CC1
G G C T A C A G C A G C A G A T G G T
C C G A T G T C G T C G T C T A C C A T G C A T
CC1
G G C T A C A G C A G C A G A T G G T
5 C C G A T G T C G T C G T C T A C C A
3
5 A G C C G A T G T C G T C G T C T A C C A T G
C A T 3
C2
T C G G C T A C A G C A G C AG A T G G T
T C G G C T A C A G C A G C AG A T G G T
CC2
C C G A T G T C G T C G T C T A C C A
T C G G C T A C A G C A G C AG A T G G T
CC2
C C G A T G T C G T C G T C T A C C A
G G C T A C A G C A G C A G A T G G T
9
Part V
10
Part V
16
1
1
3
3
4
4
11
Part VI
32
1
1
4
4
11
11
12
Part VII
13
Part VII
0
0
0
1
1
0
2
1
0
0
1
1
1
4
8
1
1
1
1
2
2
3
4
1
1
3
16
4
1
1
4
11
32
4
11
1
1
5
5
26
26
64
1
1
10
1013
10
1013
2048
1,048,555
2,097,152
1
1
20
20
