Negative training samples are also important for mining microRNAs from genome-scale data

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Negative training samples are also important for
mining microRNAs from genome-scale data

• BY Leyi
  Wei

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????

• microRNA ? microRNA precursor
• (1)microRNA????????22nt????RNA,?????????,??????
  ?????????,??microRNA?????
• (2)microRNA precursor(pre-miRNA)
  ?????microRNA? ????microRNA?pre-miRNA?????
  pre-miRNA???microRNA? ???????????,???????Pre-miRNA
  ?????????????(hairpin)???

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The pathway of microRNA in mammals

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??microRNA???

• Comparative methods
• ?????? ,?????????,? ??????microRNA
• Non -- comparative methods
• ????????????????, ????????microRNA

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?????????

• 1. ??? ? ??
• ???????????????????????
• ????????????
• ???
• ???????????????,?????????
  ?????????????????(real pre-miRNA)??? (pseudo
  pre-miRNA)????

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• ?? ????????real pre-miRNA ???
• ?? ???pseudo pre-miRNA???,??????????????????,?
  ????????????real pre-miRNA?????,????????????

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??????????????

• ?????????????????
• ?????????????????,??????????????????CDS????????
  ?,???????????????????????????

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(????)??????????
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• ROC- analysis

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????

1. ?????????,???????????????,?????????
2. ???????,??????????,???????????????????????????????
   ?????

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???????????

• Triplet-SVM classifier

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???????????

• Mirident-classifier (Table)

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Mirident-classifier (Figure)
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Our ensemble classifier based on this negative set

• Our ensemble classifier performance
• ???? ??????????,?????????????,?????????
• Feature set performance
• ???? ????????????????,???????????,??????????
  ??????

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mirnaDetect

• ?????????????,??????????,??????????????pre-miRNA??
  ?

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mirnaDetect

• ????

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????

• ??????,???????????,???????????????
• ?????????(SCI)
• (1)??? ????
• ???, ?????????????????????,
  ???,????,?????????????????????????,???????????????
  ?????,??????(???????,????????)?
• ???????????????,???????????

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• (2)?????
• ??? ???,??????????????????,????????????,???????
  ????
• (3)???????cover letter .
• ??????????

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• (4) ???????
• ?????????????,????????????????????????????????
  under review?
• ??????,?????
• ?????,???????responding author

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• (5)??????!
• Rejected OR (major)Revision
• (6)Revision
• ???????? ?????Editor?? Riviewers
  ????????????????,?????????????????manuscript??,???
  ??????????,?????The Rebuttal Letter
• ????????
• ????????

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(No Transcript)
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• ??Revised manuscript
• ?????????????,????Track Changes File?
• ???????,??????????????,??????????

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• ????!