Activities in the 1st reporting period and tasks for the 2nd period Szeged

1
Activities in the 1st reporting period and
tasks for the 2nd periodSzeged Timisoara axis
for the safe food and feedSZETISA1HURO/0901/147/
2.2.2.
Reporting period 01.02. 2011 31.05. 2011

• Erdei László
• Department of Plant Biology
• University of Szeged

1st Progress Report Meeting Timisoara, 30-31 May
2011
2
Main events since the Project Inaugural
Meeting Szeged, 2-3 February, 2011
Subsidy Contract signed Budapest, 22nd April
2011 Szeged, 29th April 2011 ERDF Contract
en route (not yet signed) Report deadlines PR-
Partner Report 15 days to VÁTI and BRECO,
respectively, in HU Szécsi Jeno, Békéscsaba ,
Tel. [phone] PPR Progress Report to JTS
(in HU Kovács Gyula, Tel. [phone]) within
120 days (in the contract 28. 09. 2011)
3
Time-frame Bars represent the periods of
activities. Locations of activities are noted
under the bars. Bold M, meetings in Szeged
Italics M, meetings in Timisora.

Reporting periods Activities 0. 01.11.2009-31.01.2011 1. 01.02.2011- 31.05.2011. 2. 01.06.2011- 30.09.2011. 3. 01.10.2011- 31.01.2012. 4. 01.02.2012- 31.05.2012.
A0 Preparation MM
A1 Management
A2 Communication M M M M M
A3 Research
Preliminary sample collection Szeged Timisoara
Procuration and installation of equipments Szeged, Timisoara , Szeged, Timisoara ,
Plant growing and sample collection Szeged, Timisoara Szeged, Timisoara
Physiological and biochemical measurements Szeged Szeged Szeged
Microbiological and toxin investigations Timsoara Timsoara Timsoara
Element analysis Szeged Szeged
Closing activities Szeged, Timisoara ,
4
General objectives The interactive effects of
abiotic, biotic and genetic factors on the
performance of wheat, maize and alfalfa plants
and yield components that may influence nutrient
quality and safety

• The two sides of the micronutrient availability
  (deficiency and excess)
• ii) The presence of microbial toxins due to
  microbial (fungal and/or bacterial) infections
  and their effects on the microelement
  accumulation (effects of toxins on channel
  activity)
• iii) Toxins and physiological/metabolic
  processes
• iv) The effects of genomic background on
  micronutrient accumulation and distribution.

5

• 1st Reporting period 01. 02. 2011 31. 05. 2011
• Deadline of the report according to the
  contract 28. 09. 2011.
• Project inaugural (Szeged) and progress report
  preparatory (Timisoara) meetings.
• Visibility activities (press and electronic
  media)
• Initialization of instrument procurements.
• Microelement analysis of plant samples collected
  in summer 2010 (during the zero period).
• Biochemical investigations on the saples of
  summer 2010 wheat flag leaves (defence enzymes).

6

• 2nd Reporting period 01. 06. 2011. 30. 09.
  2011
• Deadline of the report according to the
  contract 28. 01. 2012
• Management and communication.
• Procuration of instruments, installation,
  teaching.
• Collection wheat plant parts (flag leaves, ears)
  from Fusarium-inoculated and control palnts,
  different genotypes for element and biovchemical
  analyses.
• Physiological and in situ photosynthesys
  measurements. Determination of antioxidant
  enzymes.
• Toxicology in Timisoara.
• Progress report preparatory meeting in Szegeden.

7
Taks for the next reporting period (01.06.2011
30.09.2011
8
Activity Package 0 11/2009
present Preparation activities Preparatory
Meetings -25.11. 2009 Szeged 13.01.2010
Timisoara
Activity Package 1 2/2011 5/2012 Project
management
Project preparatory meetings 2 Szeged 1, Timisoara 1
Progress reports and closing report progress report 3, closing report 1
Closing seminars 2 Timisoara 1, Szeged 1
Improved infrastrucure in the laboratories Szeged 1,Timisoara 3 instruments
Activity Package 2 2/2011 5/2012 Communication
activities
Results - Project meetings 5 Szeged 3, Timisoara 2
Results - Publications 7 Szeged 5, Timisoara 2
Outputs - Number of participants in meetings 20 in each, altogether 100. Project Booklet, 100 pcs., Project brochure - 100pcs.
Outputs - Number of readers in newspapers N/A
Outputs - number of authors in scientific publications, number of students on courses 6 authors, 300 students
9
Activity Package 3 02/2011 05/2012 Research
and Development
1. Procuration of equipments 2-7/2011 Szeged, Timisoara

Procuration of equipments is planned in both localities to improve infrastucture. In Szeged, an inductively coupled plasma mass spectrometer (ICP-MS) is planned for the multielement analysis of microelements in environmnetal, food and feed plant samples in the region of mg/kg to pg/kg. In Timisora, the purchase of a Bioanalyzer, a UV-VIS Spectophotometer and a cooling centrifuge are necessary to improve molecular biological work.
2. Determination of microelements as affected by microbial infection or heavy metals presence . 7/2011 5/2012 Szeged, Timisoara
These will be determined in 2 wheat cultivars, 2 corn genotypes (GM and conventional) and alfalfa cultivars of different susceptibilities. Microelements will be determined in Szeged, microbial presence and toxins in Timisoara.
3. Determination of antioxidant defence mechanisms as influenced by microbial toxins 7/2011 5/2012 Szeged
The catalase, superoxide dismutase, peroxidase , glutathione S-transferase activities will be determined in the same biologicical material. In addition, glutamine syntethase iso-enzymes and Rubisco levels will be determined by 2D gel chromatography. These will be carried out in Szeged.
4. Effects of genomic background on essential and toxic microelements 7/2011 5/2012 Szeged, Timisoara
This subactivity is based on earlier results we have shown out significantly different levels of Fe, Zn, Cu and Mn in wild, ancient and cultivated wheat species in the genera Triticum and Aegilops. Alfalfa (Medicago sativa) cultivars also show different phytoremediation potential for both inorganic and organic pollutants. Besides the analysis of wheat and alfalfa cultivars, investigation for their membrane-transport mechanisms and photosynthetic activities will also be carried out.
10
Results and Outputs
Increase laboratory infrastructure 2 laboratories, Szeged 1, Timisoara 1
Results - Determination of factors influencing food (wheat ) and feed (fodder corn and alfalfa) nutrient safety and mineral composition 3 groups, mineral factors, toxin factors, genetic factors
Output - Equipments for mineral analysis and for molecular biological work Szeged, 1 ICP-MS equipment Timisoara, 3 molecular biological equipments.
Output - Mineral composition, toxins, genetic background 3 for wheat, 3 for corn and 3 for alfalfa mineral composition 1, toxins 1, genetic background 1., each, together 9
   
11
Thank you for your attention
12
(No Transcript)
13
Analysis of wheat lines resistant or sensitive
for Fusarium
Some essential and toxic elements in wheat as
determined by ICP
14
(No Transcript)
15
(No Transcript)
16
(No Transcript)