MYCOBACTERIUM%20TUBERCULOSIS%20PROTEOME

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MYCOBACTERIUM TUBERCULOSIS PROTEOME

• M. tuberculosis- intracellular pathogen
• - TB prevalent in Africa and Asia
• - 1/3 population is infected
• - 8 million new cases per year
• Complete sequence is available in SPTR
• NCBI_TaxID1773 (Rv83332) (CDC155183331)
• 4087 proteins in SPTR, 2706 are hypothetical
• 66 of proteins in SPTR uncharacterised

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Classification of hypothetical proteins
Q10401 PEPTIDASE B (EC 3.-.-.-). IPR000819
Cytosol aminopeptidase O33261 L-ASPARAGINE
PERMEASE. IPR002027 Amino acid permease Q11018
HYPOTHETICAL ABC TRANSPORTER. IPR001617 ABC
transporters family P96936 HYPOTHETICAL 54.8 KD
PROTEIN. IPR002106 Aminoacyl-transfer RNA
synthetases class-II P96255 ACETATE KINASE (EC
2.7.2.1). IPR000890 Acetate and butyrate
kinase Q10535 HYPOTHETICAL PROTEIN RV0873.
IPR001552 Acyl-CoA dehydrogenase Q10974 PROBABLE
THIOESTERASE RV2928 (EC 3.1.2.-). IPR001031
Thioesterase Q50705 ALANINE RACEMASE (EC
5.1.1.1). IPR000821 Alanine racemase Q11024
HYPOTHETICAL 67.7 KD PROTEIN. IPR001633 Domain of
unknown function P71571 HYPOTHETICAL 83.6 KD
PROTEIN. IPR000977 ATP-dependent DNA
ligase Q50720 HYPOTHETICAL 20.6 KD PROTEIN
RV3405C. IPR001647 Bacterial regulatory proteins,
TetR
M. tuberculosis proteome 3784 proteins - 2505
have InterPro matches (64.7) - Of those in
InterPro, at least 860 hypothetical can be
characterised - 33 of hypothetical proteins in
SPTR, or 22 proteome
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Proteome analysis of Mtb

• Extensive gene duplication, functional redundancy
  and diversification
• Precise function 40, some functional info 44,
  16 unknown, probably Mycobacterial specific
• 9 genome encodes PE and PPE proteins (gly rich)
• Many fatty acid metabolism proteins gt250 cf 50 in
  E.coli (reflection of cell wall)
• Has two hemoglobins for oxygen storage
• Mycobactins for iron
• Glyoxylate shunt NB for latency or persistence
  (isocitrate lyase)

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Structural information

• 1079 ORFs can be assigned to SCOP -1566 domains,
  1639 regions
• 482 (12.4) have hits in HSSP
• 9 (0.2) have hits in PDB

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Venn diagram of InterPro hits for the 3 organisms
Differences -Glycosol hydrolases -Proteases -Tra
nscription factors -Transporters -Oxygenases
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Pie charts of whole proteome analysis of 4
organisms
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Distribution of protein functions