Warm-up

1
Warm-up

• Name 3 sections of DNA within a gene and 1
  section of DNA outside of a gene
• What does gene expression mean?
• What is the OUTPUT of transcription, what is
  produced?

2
Check your understanding
Name DNA or protein? Function
Promoter
Coding sequence
Activator
Enhancer
RNA polymerase
Terminator
General transcription factors
Draw a labeled diagram with each of the elements
above.
3
Whats the evidence?

• Modeling the process of science and
  hypothesis-testing
• Using scenario of lactose intolerance and
  reporter constructs

4
Given hypothesis and conditions, predict results
Lactase gene
DNA
A T
T A
Enhancer
Promoter
Coding sequence
Enhancer
No promoter or enhancer
No enhancer
Promoter enhancer sequences
A T
T A
5
Compare results with predictions
Explain these results using our model for gene
regulation
6
How can cells have the same DNA but different
sets of proteins?

• Genes are regulated independently of one another.
• In a given cell, the expression of some genes is
  on while other genes are off. Different
  cells express different sets of genes at
  different times.

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8
CELL 2
Activators in cell
Which proteins made in cell?
Coding sequence gene A
Coding sequence gene B
Coding sequence gene C
CELL 3
Activators in cell
Which proteins made in cell?
Coding sequence gene A
Coding sequence gene B
Coding sequence gene C
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