Chapter 5: DNA, Gene Expression, and Biotechnology

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Chapter 5 DNA, Gene Expression, and Biotechnology

• Clicker Questions

by Kristen Curran, University of
Wisconsin-Whitewater
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Which answer will base pair with the following
sequence? AGTTCTCATGT

1. AGTTCTCATGT
2. ACATGAGAACT
3. TCAAGAGTACA
4. UCAAGAGUACA

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Different people can have free or attached
earlobes. The DNA that encodes for making free
or attached earlobes is called a(n) ________, and
there are two different versions of it, called
__________.

1. allele genes
2. trait alleles
3. gene trait
4. gene alleles

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Which molecule acts as a middle man between the
nucleus, where transcription occurs, and the
cytoplasm, where translation occurs?

1. DNA
2. mRNA
3. Protein
4. Choices 1 and 3 are correct.

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Classroom Catalyst

• Transcription Activity

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Which answer shows the correct orientation of the
mRNA sequence (Phosphate (5) ? Oxygen (3))
given the DNA sequence below? 5-TCATCTATCT-3

1. 5-UCAUCUAUCU-3
2. 5-AGUAGAUAGA-3
3. 5-AGAUAGAUGA-3
4. 5-AGATAGATGA-3

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Classroom Catalyst

• Translation Activity

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Translate the following mRNA 5-CGAGCAUGGGCUUUA
GG...

1. MET-GLY-PHE-ARG
2. ARG-ALA-TRP-ALA-LEU
3. GLY-PHE-ARG-VAL-ARG
4. ARG-PHE-GLY-MET-SER

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Which answer shows the mRNA transcribed from the
DNA sequence below? 5-TTA TCC TTT ACT CAT-3

1. 5-AUG AGU AAA GGA UAA-3
2. 5-AAU-AGG-AAA-UGA-GUA-3
3. 5- TTA-TCC-TTT-ACT-CAT-3
4. 5-UUA-AGG-AAA-TGA-GUA-3

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Which of the following changes (base
substitutions and insertions are underlined and
in bold) would have the most severe effect on the
protein made from the mRNA in the last
question? 5-AUG AGU AAA GGA UAA-3

1. AUG-AGU-AAG-GGA-UAA
2. AUG-AGU-AAA-GGA-UAG
3. AUG-GUA-AAG-GAU-AA
4. AUG-AGU-AAA-GGA-GUA-A

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Why can one change in a nucleotide sequence of a
gene cause a nonfunctional protein to be produced?

1. The change can cause a stop codon to occur early
   in translation and the full length protein is not
   translated.
2. A substitution can result in one incorrect amino
   acid being present in the protein which can
   affect the shape of the protein and therefore its
   function.
3. An insertion or deletion can occur early in the
   gene and result in an incorrect translation of
   the protein.
4. All of the above.

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If you were trying to identify the gene for
insulin, which particular step of the procedure
outlined below would you use?

1. Cut human DNA into small pieces with a
   restriction enzyme.
2. Amplify the DNA pieces with PCR.
3. Insert the gene into a plasmid and insert into
   bacteria.
4. Probe a gene library.

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I would want to know if my genome or that of a
loved one carries one or more copies of a gene
that can cause a genetic disease.

1. Strongly agree
2. Agree
3. Neutral
4. Disagree
5. Strongly disagree

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Which food below does not potentially contain
GMOs?

1. Nacho chips
2. Fruit juice
3. Corn flakes
4. Rice
5. All of the above.

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Using the DNA fingerprint information below,
determine which suspect was present at the crime
scene?
Crime Scene
Suspect 1
Suspect 2
Suspect 3

1. Suspect 1
2. Suspect 2
3. Suspect 3
4. All of the above.

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We should pursue cloning in animals but not
humans.

1. Strongly agree
2. Agree
3. Neutral
4. Disagree
5. Strongly disagree

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We should pursue cloning in humans for the
purpose of developing or performing medical
treatments.

1. Strongly agree
2. Agree
3. Neutral
4. Disagree
5. Strongly disagree

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