??: Developmental Biology

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?? ????? ?? Developmental Biology ???? ???
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This course aims to help the students understand
how life develops from a single fertilized egg
to an organism with complicated and integrated
functions.
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By the end of the course, the students will be
familiar with (1)The development of model
organisms including Drosophila, zebrafish, mouse
etc. (2)The important molecular and cellular
mechanisms that govern the developmental
processes. (3)How congenital diseases will occur
while normal development is perturbed.
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The frontiers of developmental biology, such as
cloning and stem cell.
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4.8 Cloned mammals, whose nuclei came from adult
somatic cells
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4.9 The kitten CC (A) is a clone produced
using somatic nuclear transfer from Rainbow (B)
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Scott F. Gilbert Developmental Biology. 8th
edition www.devbio.com ????
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The foundations of developmental
biology Observations of living organisms in
nature! (not from test tubes) The ability to
observe and record the anatomical details of an
organisms Artist as well as natural scientist
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Developmental biology in the post-genomics
era The relationship between Gene and
Development Gene expression Molecular biology
and biochemistry Knock-out and knock-in
techniques Genetics Genomics
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Studying the functions of a gene by Knock-out
technologies Homologous recombination Double-str
anded RNA (dsRNA) Antisense morpholino oligo
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4.19 Technique for gene targeting
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4.21 Use of antisense RNA to examine the roles
of genes in development
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4.22 The dsRNA-mediated gene silencing procedure
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4.23 Injection of dsRNA for E-cadherin into the
mouse zygote blocks E-cadherin expression
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Antisense Morpholino Oligonucleotide
a targeted knockout in zebrafish
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Studying the functions of a gene by Knock-in
technologies Reporter transgenic lines (For
monitoring the expression of a gene or tracing a
specific lineage. ) Chimeric mice Transgenic
animal for pharmaceutical industry
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4.17 Insertion of new DNA into embryonic cells
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4.18 Production of transgenic mice
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4.18 Production of transgenic mice
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Other approaches for understanding the function
of a gene Spatio-temporal expression RNA
localization techniques for understaning
differential gene expression
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4.12 The reverse transcriptase-polymerase chain
reaction technique for determining whether a
particular type of mRNA is present
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4.13 Microarray technique
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4.15 In situ hybridization showing the
expression of the Pax6 gene in the developing
mouse eye
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4.16 Whole-mount in situ hybridization
localizing Pax6 mRNA in early chick embryos
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4.16 Whole-mount in situ hybridization
localizing Pax6 mRNA in early chick embryos
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Model organisms of genetics and
genomics Mouse Drosophila Zebrafish C.
elegans Arabidopsis Other model organisms for
developmental biology Chick Amphibians